实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (5): 440-447.DOI: 10.12300/j.issn.1674-5817.2022.129

• 实验动物设施及管理 • 上一篇    下一篇

普通环境设施内微屏障笼具饲养和维持清洁级小鼠初探

任琦(), 李艳东, 孙强()()   

  1. 中国科学院脑科学与智能技术卓越创新中心, 上海 200031
  • 收稿日期:2022-08-19 修回日期:2022-09-21 出版日期:2022-10-25 发布日期:2022-11-04
  • 通讯作者: 孙强(1973—),男,博士,研究员,研究方向:辅助生殖与模式动物构建,实验动物管理。E-mail:qsun@ion.ac.cn。ORCID:0000-0002-3359-9465
  • 作者简介:任琦(1988—),女,本科,实验师,研究方向:实验动物大小鼠的饲养管理、设施维护。E-mail:qren@ion.ac.cn
  • 基金资助:
    中国科学院战略生物资源专项实验动物平台项目(KFJ-BRP-005);国家自然科学基金杰出青年科学基金“非人灵长类生殖与发育”(31825018)

Preliminary Study on Raising and Maintaining Clean Mice with Micro-barrier Cages in Conventional Environmental Facilities

Qi REN(), Yandong LI, Qiang SUN()()   

  1. Center for Excellence in Brain Science and Intelligent Technology, Chinese Academy of Sciences, Shanghai 200031, China
  • Received:2022-08-19 Revised:2022-09-21 Online:2022-10-25 Published:2022-11-04
  • Contact: SUN Qiang (ORCID: 0000-0002-3359-9465), E-mail: qsun@ion.ac.cn

摘要:

目的 探讨在普通环境设施内用微屏障笼具饲养和维持清洁级小鼠的可行性。 方法 在普通环境内采用正压微屏障笼具饲养和维持清洁级小鼠,其间以每个季度一次对微屏障内动物的微生物状况进行第三方抽样检测。 结果 自2020年6月开始用微屏障笼具饲养清洁级小鼠,至2022年7月文章成稿时,在连续两年的抽样检测中没有检出国家标准中要求清洁级小鼠必须排除的微生物。 结论 微屏障笼具可以在普通环境设施内实现清洁级小鼠的饲养和维持。

关键词: 微屏障笼具, 清洁级小鼠, 饲养管理

Abstract:

Objective To explore the feasibility of breeding cleaning degree mice with micro-barrier cages in conventional environmental facilities. Methods Clean-grade mice were housed and maintained in positive-pressure microbarrier cages in a general environment, during which third-party sampling of the microbial status of the animals in the microbarrier was performed on a quarterly frequency. Results From June 2020 to July 2022, no microorganisms that must be excluded by the national standard for cleaning degree mice have been detected in two consecutive years. Conclusion Micro-barrier cages can be used to raise and maintain cleaning grade mice in conventional environmental facilities.

Key words: Micro-barrier cage, Cleaning degree mice, Breeding management

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