上海地区常用近交系小鼠品系的单核苷酸多态性分型研究

doi:10.3969/j.issn.1674-5817.2017.01.006

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (1): 25-31.DOI: 10.3969/j.issn.1674-5817.2017.01.006

所属专题：实验动物资源开发与利用

上海地区常用近交系小鼠品系的单核苷酸多态性分型研究

韩琳¹, 谢建云², 杨斐³, 胡樱³, 田立立², 鲍世民¹

1.上海斯莱克实验动物有限责任公司, 上海201615;
2.上海实验动物研究中心, 上海201203;
3.复旦大学实验动物科学部, 上海200032

收稿日期:2016-09-30 出版日期:2017-02-25 发布日期:2017-02-25
作者简介:韩琳(1982-),女,硕士,实验师,研究方向:实验动物遗传质量控制,PDX肿瘤模型。E-mail:hanlin594@126.com
基金资助:
上海市科学技术委员会科研计划项目(14140900800)

The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai

HAN Lin¹, XIE Jian-yun², YANG Fei³, HU Ying³, TIAN Li-li², BAO Shi-min¹

1. Shanghai SLAC Laboratory Animal Ltd.Co., Shanghai, 201615;
2. Shanghai Laboratory Animal Center, Shanghai, 201203;
3. Fu Dan University, Shanghai, 200433

Received:2016-09-30 Online:2017-02-25 Published:2017-02-25

摘要/Abstract

摘要： 目的比较上海地区常用近交系小鼠单核苷酸多态性(SNP)位点分型情况。方法采用多重PCR-LDR检测技术,选取上海地区2家单位的10个近交系小鼠,对21条染色体上的44个SNP位点进行分型检测,同时,随机选取上海地区2个近交系品系作为双盲样本,对分型方案进行验证,差异位点利用测序法进行验证。结果同一来源的10个近交系小鼠,同品系间SNP分型结果完全一致;不同种群来源6个品系SNP分型结果提示, BALB/c,FVB, DBA/2和C3H/He在44个位点上分型结果均相同,CBA和C57BL/6分别在7个和2个位点存在差异; 差异位点经测序验证结果与SNP分型方案一致。结论上海地区常用近交系小鼠中,同一来源的品系中SNP遗传质量具有良好稳定性和一致性,不同种群来源的相同品系间SNP分型存在差异。

关键词: 近交系小鼠, 遗传监测, 单核苷酸多态性(SNP)分型

Abstract: Objective To analyze the single nucleotide polymorphism (SNP) genotyping of inbred mice from Shanghai. Methods 44 SNP loci on 21 chromosomes of 10 inbred mice strains from 2 laboratory animal companies of Shanghai were detected by the PCR-LDR genotyping panels, and the other 2 inbred mice strains were selected as the double blind samples for verification. And discrepancy locus were verified by sequencing. Results The SNP genotyping results of all the samples from the same inbred mice strains were homomorphism, but 6 inbred mice strains from different populations(BALB/c, FVB, DBA/2 and C3H/He) were polymorphism with 7 and 2 discrepancy locus respectively. The sequencing results to verify the discrepancy locus were the same as the SNP genotyping results. Conclusions The SNP genotyping was very stable and consistent in inbred mice strains from the same population in Shanghai, and the SNP genotyping of the same inbred mice strains from different populations was polymorphism.

Key words: Inbred mice, Genetic monitoring, Single nucleotide polymorphism (SNP) genotyping

中图分类号:

Q95-33

韩琳, 谢建云, 杨斐, 胡樱, 田立立, 鲍世民. 上海地区常用近交系小鼠品系的单核苷酸多态性分型研究[J]. 实验动物与比较医学, 2017, 37(1): 25-31.

HAN Lin, XIE Jian-yun, YANG Fei, HU Ying, TIAN Li-li, BAO Shi-min. The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 25-31.

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上海地区常用近交系小鼠品系的单核苷酸多态性分型研究

The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai

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