近交系侏儒大鼠的培育及其主要生物学特性测定

doi:10.3969/j.issn.1674-5817.2016.04.010

摘要/Abstract

摘要： 目的建立侏儒大鼠近交系。方法选择Wistar大鼠群中出现的疑似侏儒症突变动物, 采用“全同胞兄妹交配”培育和维持,测定分析其各年龄段生长发育、繁殖性能、脏器重量、血液生理(12项)生化(12项)等主要指标,作为品系参考值,并与原种Wistar大鼠进行比较。结果至2016年2月, 侏儒大鼠实现连续近交达22~23代,品系暂时命名为“侏儒大鼠”(SDWR)。SDWR幼年、青年及成年体质量分别仅为相应年龄段Wistar的45.7%, 37.5%, 36.2%(♂)和49.7%, 41.7%,40.3%(♀)（P<0.01); SDWR青年期体长、尾长分别仅为Wistar大鼠的77.5%、72.3%(♂)和73.8%、68.9%(♀)(P<0.01),即随年龄增长SDWR与原种Wistar体质量和体尺差异显著增加。SDWR青年及成年期主要脏器(心、肝、脾、肺、肾、睾丸/输卵管)重量显著低于同年龄Wistar大鼠(P<0.01)。SDWR主要繁殖性能(产仔数、窝重、离乳率)均显著低于原种Wistar大鼠(P<0.01）。血液生理生化主要指标SDWR与Wistar差异不明显。结论通过长期近交培育成的SDWR在生长发育、体尺、繁殖性能、脏器重量等方面均明显体现侏儒症状,并能稳定遗传。

关键词: 侏儒症, 近交系, 侏儒大鼠(SDWR), 体质量, 体尺, 脏器重, 血液生理生化

Abstract: Objective To breed the inbred strain of dwarf rat and determine the main biological characteristics. Methods The spontaneous mutant litters looks like dwarfism from the Wistar conlony was bred and maintained by brother×sister (B×S) mating system for 22-23 progenies and nominatured as spontaneous dwarfism rat (SDWR) according the standard. As reference of strain, indexes of growth and development, body measurements, reproductive performance, organ weight, serum biochemical (12 items) and hematology (12 items) about dwarf rat at different age stages were measured, and be compared with background Wistar rat. Results Up to February 2016, the dwarf rat was inbreded continuously for 22-23 generations, and named as SDWR temporarily. The weight of weanling, youth and adult individuals in SDWR was respectively only about 45.7%, 37.5%, 36.2%(♂) and 49.7%, 41.7%, 40.3%(♀) of the same age Wistar rats (P<0.01). The body and tail length of youth and adult individuals in SDWR is respectively 77.5%, 72.3% (♂) and 73.8%, 68.9%(♀) of Wistar rats (P<0.01). The weights of major organs (heart, liver, spleen, lung, kidney, testis or oviduct tubes) in youth and adult SDWR are significantly lower than that of the same ages Wistar rats (P<0.01). Conclusions Through 22-23 progenies inbreeding, the established inbreeding strain of SDWR exhibited obvious dwarfism symptoms in growth, body measurements, reproductive performance and organs weight, and has stable heredity. The rat may be useful as animal models for the study of inherited dwarfism in human.

Key words: Dwarfism, Inbred strain, Spontaneous dwarfism rat (SDWR), Body mass, Body size, Organ weight, Physiological and biochemical indexes of blood

中图分类号:

Q95-33

姚菊芳, 华征宇, 王斌, 孙志锴, 戴慧莉. 近交系侏儒大鼠的培育及其主要生物学特性测定[J]. 实验动物与比较医学, 2016, 36(4): 295-300.

YAO Ju-fang, HUA Zheng-yu, WANG Bin, SUN Zhi-kai, DAI Hui-li. Breeding of Inbred Dwarf Rats and its Main Biological Characteristics[J]. Laboratory Animal and Comparative Medicine, 2016, 36(4): 295-300.

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