两种小鼠肺癌模型的构建及Micro PET-CT观察

doi:10.3969/j.issn.1674-5817.2019.01.008

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (1): 39-45.DOI: 10.3969/j.issn.1674-5817.2019.01.008

两种小鼠肺癌模型的构建及Micro PET-CT观察

沈艳^1,3, 王韵², 张汝², 阮铮², 毛建华²

1.上海交通大学医学院附属瑞金医院实验医学研究中心, 上海200025;
2.医学基因组学国家重点实验室, 上海血液学研究所,上海交通大学医学院附属瑞金医院, 上海, 200025;
3.南京农业大学, 动物医学院, 南京, 210095

收稿日期:2018-06-28 出版日期:2019-02-25 发布日期:2021-01-29
作者简介:沈艳(1977-),女,硕士,高级兽医师,从事实验动物管理和人类疾病动物模型研究。E-mail:35840478@qq.com
基金资助:
国家自然科学基金(81101721), 上海市浦江人才计划项目(16PJ1406100), 上海市自然科学基金(16ZR1421000)

Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT

SHEN Yan^1,3, WANG Yun², ZHANG Ru², RUAN Zheng², MAO Jian-hua²

1. Research Center for Experimental Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2. State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025,China;
3. College of Veterinary Medicne, Nanjing Agricultural University, Nanjing 210095, China

Received:2018-06-28 Online:2019-02-25 Published:2021-01-29

摘要/Abstract

摘要： 目的比较尾静脉注射与肋间隙肺部原位注射人源非小细胞肺癌(NSCLC)细胞制备肺癌小鼠模型肿瘤形成情况的差异。方法使用NSCLC株NCI-H1975细胞,通过尾静脉注射以及经肋间隙肺部原位注射的方法制备NOD-SCID小鼠肺癌模型,于造模后15 d,应用小动物PET-CT技术观察小鼠肺部肿瘤形成情况; 解剖小鼠,检测肺部病理改变。结果造模15 d后,经尾静脉注射NCI-H1975细胞的小鼠在肺部不能检测到肿瘤,而经肋间隙肺部原位注射NCI-H1975细胞的小鼠能够在肺部检测到明显的肿瘤形成。结论肋间隙肺部原位注射肺癌细胞制备的肺癌小鼠模型肺部肿瘤形成均一、稳定、可重复、高效,是较好制备原位肺癌模型的方法。

关键词: 原位肺癌, 小鼠模型, 小动物PET-CT, 尾静脉注射, 肋间隙注射

Abstract: Objective To compare the difference of two kinds of lung cancer models in mouse, which were established by injecting with the human non-small cell lung cancer (NSCLC) cells through tail vein or orthotopically inoculating in the lung using a microsyringe through the intercostals space. Methods The NCI-H1975 cells of NSCLC were injected into the NOD-SCID mouse via tail vein or intercostals space. After 15 days, mice were scanned by micro PET-CT to monitor the tumor growth and metastasis. Then mice were sacrificed and their lungs were dissected, fixed with phosphate-buffered neutral formalin and prepared for standard histological examination. Results Mice that were injected with the NCI-H1975 cells through tail vein for 15 days did not form tumor nodules on the lung. However, the mice that were orthotopically inoculated in the lung through intercostals space significantly had tumor nodules in their lungs. Conclusion A NOD-SCID mouse lung tumor model can be successfully established by transplanting NCI-H1975 cells into the lung through intercostals space. This model is simple, repeatable, and efficient.

Key words: Mouse orthotopic lung cancer model, Micro PET-CT, Tail intravenous injection

中图分类号:

Q95-33

沈艳, 王韵, 张汝, 阮铮, 毛建华. 两种小鼠肺癌模型的构建及Micro PET-CT观察[J]. 实验动物与比较医学, 2019, 39(1): 39-45.

SHEN Yan, WANG Yun, ZHANG Ru, RUAN Zheng, MAO Jian-hua. Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT[J]. Laboratory Animal and Comparative Medicine, 2019, 39(1): 39-45.

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两种小鼠肺癌模型的构建及Micro PET-CT观察

Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT

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