关键词: 盐敏感性高血压, 硝苯地平, 降压药, 小鼠模型

Abstract: Objective     To establish a mouse model of salt-sensitive hypertension, and to verify the effectiveness of the model using by nifedipine, an antihypertensive drug. Methods    C57BL/6J mice were divided into the normal control group, high-salt diet group and high-salt diet with nifedipine group. The blood pressure change of mice was measured by non-invasive blood pressure system (CODA system). The biochemical indexes of liver function, renal function, blood lipid, blood sugar and ions were measured by the blood biochemical analyzer, and the histological changes of the liver, kidney and carotid artery were observed after hematoxylin-eosin staining. Results    Compared with the normal mice, the systolic and diastolic blood pressure in the high-salt diet group increased significantly (both P<0.01), and those in the high-salt diet with nifedipine group decreased significantly (both P<0.01), and there were statistically significant differences. The liver function indexes (aspartate transaminase, alkaline phosphatase, albumin, total serum protein, and direct bilirubin), blood lipid index (cholesterol), renal function index (uric acid) and ionic levels (Ca, Mg) in high-salt diet group were significantly different from the control group, and those in the high-salt diet with nifedipine group were gradually recovered after nifedipine intervention. The pathological histology showed that the liver and kidney of the high-salt diet group had different degrees of injury. Conclusion    High-salt diet can successfully establish a mice model of salt-sensitive hypertension, and nifedipine can effectively lower the blood pressure of the model mice.

Key words: Salt-sensitive hypertension, Nifedipine, Hypotensor, Mouse model