乙型肝炎急性感染小鼠模型的建立

doi:10.3969/j.issn.1674-5817.2012.01.010

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (1): 38-42.DOI: 10.3969/j.issn.1674-5817.2012.01.010

乙型肝炎急性感染小鼠模型的建立

汤宏斌¹, 邬开朗², 程腊英¹, 蒋明森³

1.武汉大学动物实验中心, 武汉430071;
2.武汉大学生命科学学院, 武汉430072;
3.武汉大学基础医学院, 武汉430071

收稿日期:2011-06-22 出版日期:2012-02-25 发布日期:2012-02-25

A New Mouse Model of Acute Hepatitis B Virus Infection

TANG Hong-bin¹, WU Kai-lang², CHENG La-ying¹, JIANG Ming-sen³

1. Laboratory Animal Center, Wuhan University, Wuhan 430071, China;
2. State Key Laboratory of Virology, Wuhan University, Wuhan 430070, China;
3. School of medicine, Wuhan University, Wuhan 430071, China

Received:2011-06-22 Online:2012-02-25 Published:2012-02-25
About author:TANG Hong-bin (1971-), men, M. D student. E-mail: tanghb1971@163.com

摘要/Abstract

摘要： 目的通过尾静脉注射感染性小鼠血清的方法建立一种简单复制乙型肝炎急性感染的小鼠模型。方法收集通过水压法复制第3天的小鼠血清,使用不同剂量尾静脉注射感染同系小鼠。分别在不同的时间阶段检测血清中的HBsAg和HBeAg,肝组织中的HBcAg和血清中HBV的DNA。结果注射0.1 ml血清的感染组的检测结果均高于0.2 ml组。结论通过注射感染血清可以成功地复制急性小鼠感染模型。该模型复制方法中,感染的结果与注射感染血清的剂量无关。

关键词: 小鼠模型, hepatitits B Virus, 感染血清, 静脉注射

Abstract: Objective To establish an acute HBV mouse model by tail vein injection of infectious sera. Methods Mice sera at 3 days after hydrodynamics-based transfection was collected and injected into the tail vein of BALB/c mice with volume of 0.1ml and 0.2ml respectively. The serum was slowly administrated within 10-12s. DNA levels of HBV e antigen (HBeAg), HBV surface antigen (HBsAg), HBV core antigen (HBcAg) and HBV were determined and compared before and after infectious sera injection. Results The levels of serum HBsAg, HBeAg, HBV DNA and liver HBcAg in mice injected with 0.1ml viral sera were higher than that injected with 0.2ml and hydrodynamic injected pBule-HBV. Conclusions The method of intravenous injection with low dose of infectious sera could be used to establish a mouse model of acute HBV infection.

Key words: Mouse model, Hepatitits B Virus, Infectious serum, Intravenous injection

中图分类号:

Q95-33

汤宏斌, 邬开朗, 程腊英, 蒋明森. 乙型肝炎急性感染小鼠模型的建立[J]. 实验动物与比较医学, 2012, 32(1): 38-42.

TANG Hong-bin, WU Kai-lang, CHENG La-ying, JIANG Ming-sen. A New Mouse Model of Acute Hepatitis B Virus Infection[J]. Laboratory Animal and Comparative Medicine, 2012, 32(1): 38-42.

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乙型肝炎急性感染小鼠模型的建立

A New Mouse Model of Acute Hepatitis B Virus Infection

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