睡眠剥夺对大鼠肝脏铁代谢的影响

doi:10.3969/j.issn.1674-5817.2014.03.007

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (3): 205-209.DOI: 10.3969/j.issn.1674-5817.2014.03.007

睡眠剥夺对大鼠肝脏铁代谢的影响

管宇, 严晓丽, 王国华, 张晓峰

上海市中医医院实验中心, 上海200071

收稿日期:2014-04-15 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:管宇(1976-), 女, 博士, 主管技师, 从事铁代谢紊乱性疾病分子机制研究,E-mail: yuguan1976@163.com
基金资助:
上海市自然科学基金(13ZR1440000)

Effects of Sleep Deprivation on Rat Liver Iron Metabolism

GUAN Yu, YAN Xiao-li, WANG Guo-hua, ZHANG Xiao-feng

Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated TCM University, Shanghai 200071, China

Received:2014-04-15 Online:2014-06-25 Published:2014-06-25

摘要/Abstract

摘要： 目的探讨睡眠剥夺对大鼠肝脏铁代谢的影响及相关分子机理。方法 20只雄性SD大鼠(180±10 g)随机分为对照组(CG)和睡眠剥夺组(SDG)。CG大鼠正常饲养,SDG大鼠采用改良多平台睡眠剥夺法建立模型。睡眠剥夺5 d后,采用比色法检测血清、肝组织铁离子浓度,实时荧光定量PCR检测肝组织铁调素基因HAMP及IL-6、IL-1 mRNA表达水平,Western-Blot检测肝组织铁蛋白、磷酸化STAT3蛋白表达。结果与CG组相比,SDG大鼠血清铁离子降低(P<0.05),而肝脏内的铁离子浓度升高(P<0.01); SDG大鼠肝组织HAMP、IL-6和IL-1 mRNA表达量分别升高1.1、3.2和1.7倍(P值均小于0.01); SDG大鼠肝脏组织铁蛋白和pSTAT3蛋白表达显著高于对照组(P<0.01)。结论睡眠剥夺可能通过IL-6-hepcidin轴导致大鼠机体铁代谢紊乱。

关键词: 睡眠剥夺, 肝脏, 铁代谢, 铁调素, 白介素6

Abstract: Objective To explore the influence of sleep deprivation on rat liver iron metabolism and related molecular mechanism. Methods Twenty Sprague-Dawley male rats (weight about 180 g) were randomly divided into normal control group (CG) and sleep deprivation group (SDG). The SD model was induced in rats by improved multi-platform sleep deprivation method. After 5 days’ sleep deprivation, the colorimetry was used to detect the iron ion concentration in rat’s serum and liver tissue samples; the expression of HAMP, IL-6 and IL-1 mRNA in rat’s liver was observed by real-time fluorescent quantitative PCR. The expression of hepatic ferritin and STAT3 protein was detected by Western Blot. Results Compared with CG group, serum iron level in SDG rats reduced (P<0.05), while liver iron concentration was higher (P<0.01); the expression of HAMP, IL - 6 and IL - 1 mRNA in SDG liver tissue increased by 1.1, 3.2 and 1.7 times respectively (P<0.01); the expression of ferritin and phosphorylated STAT3 protein in SDG liver tissue significantly higher than control group (P<0.01). Conclusion Sleep deprivation disturb iron metabolism in rats through IL - 6 - hepcidin axis.

Key words: Sleep deprivation, Liver, Iron metabolism, Hepcidin, Interleukin-6

中图分类号:

Q95-33

管宇, 严晓丽, 王国华, 张晓峰. 睡眠剥夺对大鼠肝脏铁代谢的影响[J]. 实验动物与比较医学, 2014, 34(3): 205-209.

GUAN Yu, YAN Xiao-li, WANG Guo-hua, ZHANG Xiao-feng. Effects of Sleep Deprivation on Rat Liver Iron Metabolism[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 205-209.

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睡眠剥夺对大鼠肝脏铁代谢的影响

Effects of Sleep Deprivation on Rat Liver Iron Metabolism

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