裸鼹鼠肝脏显微结构与超微结构观察

doi:10.3969/j.issn.1674-5817.2013.05.011

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (5): 373-377.DOI: 10.3969/j.issn.1674-5817.2013.05.011

裸鼹鼠肝脏显微结构与超微结构观察

袁子彦¹, 赵懿宁², 张璐¹, 赵善民¹, 林丽芳¹, 汤球¹, 孙伟¹, 余琛琳¹, 蔡丽萍¹, 徐晨¹, 崔淑芳¹

1.第二军医大学实验动物中心, 上海200433;
2.上海市杨浦区精神卫生中心, 上海200090

收稿日期:2013-07-15 出版日期:2013-10-25 发布日期:2013-10-25
作者简介:袁子彦(1984-), 女, 硕士, 助教,E-mail: yuanziyanyy@163.com
基金资助:
上海市科技发展基金项目(No. 11140901700)与 (No.12140900200)联合资助

Observation on Microstructure and Ultrastructure of Liver in Naked Mole Rat

YUAN Zi-yan¹, ZHAO Yi-ning², ZHANG Lu¹, ZHAO Shan-min¹, LIN Li-fang¹, TANG Qiu¹, Sun Wei¹, YU Chen-ling¹, CAI Li-ping¹, XU Chen¹, CUI Shu-fang¹

1. Laboratory Animal Center, Second Military Medical University, Shanghai 200433, China;
2. Yangpu District Mental Health center of Shanghai, Shanghai 200090, China

Received:2013-07-15 Online:2013-10-25 Published:2013-10-25

摘要/Abstract

摘要： 目的观察和分析裸鼹鼠肝脏的形态结构与组成。方法采集裸鼹鼠肝脏经石蜡切片和苏木精-伊红染色,光学显微镜下观察裸鼹鼠肝脏的显微结构,以及经冰冻超薄切片和染色后,通过透射电镜观察和分析裸鼹鼠肝脏脏的超微结构。结果光镜观察发现裸鼹鼠肝脏血管管腔直径较大,而且血管数量丰富; 肝细胞体积略大,胞质中没有聚集成簇的糖原成分,肝细胞成索状分布,其间血窦非常丰富,而胆管不发达; 透射电镜观察发现裸鼹鼠肝细胞处于旺盛的细胞合成阶段,线粒体和内质网数量丰富,糖原含量较少,溶酶体和自噬体也很丰富。结论裸鼹鼠肝脏具有旺盛的代谢功能、解毒能力、以及自我降解受损蛋白和细胞器的能力。

关键词: 裸鼹鼠, 肝脏, 显微结构, 超微结构, 功能

Abstract: Objective To observe and analyze the morphological structure and composition of the liver in naked mole rats. Method The liver of naked mole rat was isolated from anaesthetic naked mole rat and then made to paraffin section and hematoxylin-eosin(HE) staining, and the tissue structure was observed under common microscope.After freezing ultrathin sectioning and staining, the ultramicroscopic structure of the liver were observed by transmission electron microscope. Result The naked mole rats liver is rich in blood vessels,and the hepatic cell is larger than mice but glycogen is scarce under optical microscope. Transmission electron microscope show naked mole rat is rich in such organelles that involve in protein synthesis, such as mitochondria and rough endoplasmic reticulum. Besides,naked mole rat hepatic cells have signifficent number of smooth endoplasmic reticulum , lysosomal and autophagosome. Conclusion Naked mole rats liver has strong ability of metabolism, detoxification, and the ability to degrade damaged proteins and organelles.

Key words: Naked mole rat, Liver, Microstructure, Ultrastructure, Founction

中图分类号:

Q95-33

袁子彦, 赵懿宁, 张璐, 赵善民, 林丽芳, 汤球, 孙伟, 余琛琳, 蔡丽萍, 徐晨, 崔淑芳. 裸鼹鼠肝脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5): 373-377.

YUAN Zi-yan, ZHAO Yi-ning, ZHANG Lu, ZHAO Shan-min, LIN Li-fang, TANG Qiu, Sun Wei, YU Chen-ling, CAI Li-ping, XU Chen, CUI Shu-fang. Observation on Microstructure and Ultrastructure of Liver in Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(5): 373-377.

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裸鼹鼠肝脏显微结构与超微结构观察

Observation on Microstructure and Ultrastructure of Liver in Naked Mole Rat

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