一例干扰素受体缺失小鼠自发未成熟型睾丸畸胎瘤诊断

doi:10.12300/j.issn.1674-5817.2024.070

摘要/Abstract

摘要：

目的观察干扰素受体缺失小鼠（AG129）睾丸自发性肿瘤，为该品系小鼠的应用研究提供依据。方法 AG129小鼠种群饲养于SPF屏障环境中，正常饲养繁殖，在离乳的3周龄小鼠中发现其中一只雄性小鼠睾丸异常肿大，同窝内其他小鼠无相似情况，对该例小鼠自发的睾丸肿瘤进行持续观察，于9周龄时对小鼠安乐死，并解剖，观察肿瘤组织大体形态并取材，肿瘤组织经石蜡切片后进行HE染色。结果小鼠经过持续数周观察发现，肿瘤组织随小鼠日龄增长逐渐增大；解剖后肿瘤组织被包囊包裹，表面光滑，切开包囊后，肿瘤内组织形态不规则，部分质地坚硬，部分柔软；HE染色结果显示，肿瘤组织由多胚层组织发育而成，内部有神经组织、骨组织、肌肉组织和脂肪组织等，形态结构不规则。结论结合肿瘤发生发展，大体形态结构，病理切片HE染色结果，初步诊断该例小鼠睾丸肿块为小鼠未成熟型畸胎瘤。

关键词: 干扰素受体, 小鼠, 睾丸, 未成熟型畸胎瘤

Abstract:

Objective To observe the spontaneous testicular tumors in interferon receptor-deficient mice (AG129) and provide a basis for further research using this mouse strain. Methods The AG129 mouse population was bred in an SPF barrier environment and reproduced normally. Among the 3-week-old weaned mice, we found that one male mouse had an abnormally enlarged testis, while none of the other mice in the same litter exhibited a similar condition. The spontaneous testicular tumor in this mouse was continuously monitored. The mouse was euthanized and dissected at 9 weeks of age. The gross morphology of the tumor was observed, and the tissue was then embedded in paraffin and sectioned for hematoxylin and eosin (HE) staining. Results After several weeks of observation, the tumor tissue gradually increased in size as the mice aged. Upon dissection, the tumor tissue was encapsulated in a smooth capsule. After opening the capsule, the tumor tissue was irregular in shape, with some areas being hard and others soft. The results of HE staining showed that the tumor tissue developed from tissues derived from multiple germ layers, with nerve tissue, bone tissue, muscle tissue, and adipose tissue inside, exhibiting an irregular morphological structure. Conclusion Based on the tumor development, gross morphological structure, and the HE staining results of the pathological sections, the tumor in this mouse was preliminarily diagnosed as an immature teratoma.

Key words: Interferon receptor, Mouse, Testicle, Immature teratoma

中图分类号:

R737

赵赫,张帆,肖宇宙,等. 一例干扰素受体缺失小鼠自发未成熟型睾丸畸胎瘤诊断[J]. 实验动物与比较医学, 2024, 44(6): 691-694. DOI: 10.12300/j.issn.1674-5817.2024.070.

ZHAO He,ZHANG Fan,XIAO Yuzhou,et al. Preliminary Diagnosis and Characterization of a Spontaneous Immature Testicular Teratoma in an Interferon Receptor-Deficient Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 691-694. DOI: 10.12300/j.issn.1674-5817.2024.070.

图/表 3

图1 小鼠肿瘤在体外观的生长变化注：A，4周龄时大小；B，6周龄时大小；C，8周龄时大小。

Figure 1 Growth changes of the tumor in the mouseNote：A， Tumor size at 4 weeks of age； B， Tumor size at 6 weeks of age； C， Tumor size at 8 weeks of age.

图2 小鼠肿瘤大体解剖观察注：A，瘤体囊性组织结构；B，瘤体实体组织结构。

Figure 2 General anatomical observation of the tumor in the mouseNote：A，Cystic tissue structure of the tumor；B，Solid tissue structure of the tumor.

图3 小鼠肿瘤组织学观察（HE染色）注：A，结缔组织（标尺=500 μm）；B，骨组织（标尺=100 μm）；C，神经组织（标尺=50 μm），黑色箭头指示部分神经细胞位置；D，肌肉组织（标尺=100 μm）。

Figure 3 Histological observation of the tumor in the mouse （HE staining）Note：A，Connective tissue （×40， scale bar = 500 μm）； B，Bone tissue （×100， scale bar = 100 μm）； C，Nerve tissue （×400， scale bar = 50 μm）， with the black arrow indicating the location of nerve cells； D，Muscle tissue （×100， scale bar = 100 μm）

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