几种常用免疫缺陷小鼠的血液学及血液淋巴细胞亚群的测定与比较分析

doi:10.12300/j.issn.1674-5817.2020.123

实验动物与比较医学 ›› 2021, Vol. 41 ›› Issue (2): 155-160.DOI: 10.12300/j.issn.1674-5817.2020.123

所属专题：实验动物资源开发与利用

几种常用免疫缺陷小鼠的血液学及血液淋巴细胞亚群的测定与比较分析

谈小倩, 瞿伟, 李亮, 钱珍, 顾坚忠, 徐平

上海吉辉实验动物饲养有限公司,上海 201103

收稿日期:2020-08-19 修回日期:2021-03-01 出版日期:2021-04-25 发布日期:2021-04-30
作者简介:谈小倩(1981—), 女, 兽医师, 研究方向: 实验动物使用和管理。E-mail: xiaoqian_tan@163.com

Determination and Comparative Analysis of Hematological Indexes and Percentage of Lymphocyte Subsets in Several Immunodeficient Mice

TAN Xiaoqian, QU Wei, LI Liang, QIAN Zhen, GU Jianzhong, XU Ping

Shanghai Jihui Laboratory Animal Care Co,.Ltd, Shanghai 201103, China

Received:2020-08-19 Revised:2021-03-01 Online:2021-04-25 Published:2021-04-30

摘要/Abstract

摘要： 目的测定与比较分析几种常用免疫缺陷小鼠的血液学（血常规与血清生化）指标和血液淋巴细胞亚群百分率,为肿瘤、免疫学等研究和应用提供基础数据。方法（1） BALB/cA-nude、SCID、NOD-SCID和NPSG共4个免疫缺陷小鼠品系,每品系取6周龄小鼠各20只（雌雄各半）,同周龄近交系BALB/cA小鼠作对照,采血后测定血常规（30项）和血清生化（25项）指标;（2）4种免疫缺陷小鼠6周龄,每品系雌雄随机各取2只,用流式细胞仪检测分析淋巴细胞亚群,同周龄近交系BALB/cA小鼠作对照。结果（1）免疫缺陷小鼠的白细胞数（WBC）、淋巴细胞数（LYMPH）、淋巴细胞百分率（LYMPH%）和中性粒细胞数（NEUT）低于BALB/cA小鼠（P<0.01）,中性粒细胞百分率（NEUT%）和网织红细胞数高于BALB/cA小鼠（P<0.05,P<0.01）;SCID、NOD-SCID、NPSG小鼠的WBC、LYMPH和LYMPH%显著低于BALB/cA-nude小鼠（P<0.05）,NEUT%高于BALB/cA-nude小鼠（P<0.05）。（2）NOD-SCID和NPSG小鼠的血糖、三酰甘油水平低于BALB/cA小鼠（P<0.01）,而尿酸水平相对偏高（P<0.05）。（3）BALB/cA-nude小鼠T细胞严重缺失,B细胞和自然杀伤（NK）细胞活性降低;SCID和NOD-SCID小鼠T细胞缺失,B细胞和NK细胞活性降低更加明显;NPSG小鼠T细胞、B细胞和NK细胞均表现为缺失。结论 BALB/cA-nude、SCID、NOD-SCID和NPSG小鼠的血液学性状和血液淋巴细胞亚群的百分率均符合相应免疫缺陷小鼠的品系特征。

关键词: 免疫缺陷小鼠, 血液学, 血清生化学, 淋巴细胞亚群

Abstract: Objective To determine and compare the hematological indexes (blood biochemical and physiological indexes) and the percentage of lymphocyte subsets in immunodeficient mice, providing basic data for tumor and immunology research applications. Methods (1) A total of 4 immunodeficient mouse strains, BALB/cA-nude, SCID, NOD-SCID, and NPSG, were included. Twenty mice (6-week-old, half male and half female) were analyzed. Blood biochemical (25 items) and physiological indexes (30 items) were determined and compared with those of BALB/cA-nude mice of the same age. (2) Four strains of immunodeficient mice, 4 mice (6-week-old, half male and half female) were randomly selected and analyzed with flow cytometry. Their symphocyte subsets were compared with those of BALB/cA-nude mice of the same age. Results (1) The white blood cell (WBC) count, lymphocyte (LYMPH) count, lymphocyte percentage (LYMPH%), and neutrophil count of immunodeficient mice were lower than those of BALB/cA mice (P<0.01). In contrast, the neutrophil percentage (NEUT%) and reticulocyte count were higher than those of BALB/cA mice (P<0.05, P<0.01). Furthermore, the levels of WBC, LYMPH and LYMPH% of SCID, NOD-SCID, and NPSG mice were significantly lower than those of BALB/cA-nude mice, while NEUT% was higher than that of BALB/cA-nude mice (P<0.05). (2) The blood glucose and triglyceride levels in NOD-SCID and NPSG mice were lower than those in BALB/cA mice (P<0.01), while uric acid level was higher than that of BALB/cA mice (P<0.05). (3) BALB/cA mice had no T cells and low activity of B cells and natural killer (NK) cells. SCID and NOD-SCID mice had no T cells and lower activity of B cells and NK cells than BALB/cA-nude mice. NPSG mice had no T, B, and NK cells. Conclusion The blood biochemical and physiological indexes and the percentage of blood lymphocyte subsets in BALB/cA-nude, SCID, NOD-SCID and NPSG mice are consistent with the characteristics of corresponding immunodeficient mouse strains.

Key words: Immunodeficient mice, Hematology, Serum biochemistry, Lymphocyte subsets

中图分类号:

Q95-33
R-332

谈小倩, 瞿伟, 李亮, 钱珍, 顾坚忠, 徐平. 几种常用免疫缺陷小鼠的血液学及血液淋巴细胞亚群的测定与比较分析[J]. 实验动物与比较医学, 2021, 41(2): 155-160.

TAN Xiaoqian, QU Wei, LI Liang, QIAN Zhen, GU Jianzhong, XU Ping. Determination and Comparative Analysis of Hematological Indexes and Percentage of Lymphocyte Subsets in Several Immunodeficient Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(2): 155-160.

图/表 3

参考文献

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几种常用免疫缺陷小鼠的血液学及血液淋巴细胞亚群的测定与比较分析

Determination and Comparative Analysis of Hematological Indexes and Percentage of Lymphocyte Subsets in Several Immunodeficient Mice

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