光化学法与线栓法制作局灶性脑梗死大鼠模型的比较研究

doi:10.12300/j.issn.1674-5817.2021.095

实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (1): 48-53.DOI: 10.12300/j.issn.1674-5817.2021.095

光化学法与线栓法制作局灶性脑梗死大鼠模型的比较研究

娄惠娟¹(), 张红石², 王宇峰³, 贾萌⁴, 丛德毓¹^,³()

^1.长春中医药大学针灸推拿学院, 长春 130117
^2.长春中医药大学护理学院, 长春 130117
^3.长春中医药大学附属医院推拿科, 长春 130021
^2.长春新产业光电技术有限公司, 长春 130103

收稿日期:2021-05-10 修回日期:2021-09-18 出版日期:2022-02-25 发布日期:2022-02-25
通讯作者: 丛德毓（1961—），男，教授，研究方向：中医手法治疗脏腑疾病的临床与作用机理研究。E-mail： congdeyu666@sina.com
作者简介:娄惠娟（1990—），女，博士研究生，研究方向：中医手法治疗脏腑疾病的临床与作用机制研究。Email： 1240740144@qq.com
基金资助:
国家重点研发计划项目：中风病中医康复理论与结局评价方法研究(2018YFC1706006)

Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model

Huijuan LOU¹(), Hongshi ZHANG², Yufeng WANG³, Meng JIA⁴, Deyu CONG¹^,³()

^1.College of Acupuncture and Tuina, Changchun University of Traditional Chinese Medicine, Changchun 130117, China
^2.School of Nursing, Changchun University of Traditional Chinese Medicine, Changchun 130117, China
^3.Department of Tuina, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021, China
^4.Changchun New Industry Optoelectronics Technology Co. , Changchun 130103, China

Received:2021-05-10 Revised:2021-09-18 Online:2022-02-25 Published:2022-02-25
Contact: CONG Deyu, E-mail: congdeyu666@sina.com

摘要/Abstract

摘要：

目的　本研究探讨光化学法和线栓法两种不同的局灶性脑梗死动物模型制作方法，通过调控不同实验参数，分析不同造模方法所致实验动物梗死体积与行为学之间的相关性。方法　将64只300～330 g雄性SD大鼠随机分为假手术组、光化学组及线栓法致大脑中动脉阻塞（middle cerebral artery occlusion，MCAO）组，术后24 h对大鼠进行神经功能评分及行为学测试，然后麻醉处死取脑组织，进行2，3，5-三苯基氯化四氮唑（2，3，5-triphenyltetrazole chloride，TTC）染色，评估大鼠脑梗死体积。结果　光化学组和MCAO组SD大鼠脑组织均有体积不等的梗死灶。MCAO模型缺血部位涉及半脑大部分皮质、纹状体、丘脑、海马和脑室下带；神经功能评分及平衡木实验评分与梗死体积有良好的相关性。光化学法模型梗死灶在大脑皮层；神经行为异常与皮层梗死位置具有良好的相关性，与梗死体积之间的相关性相对较差。结论　线栓法建立MCAO模型后可观察到动物行为学评分与脑梗死体积密切相关；光化学法造模产生特定部位的病变和特定的行为变化，其行为学结果与梗死体积的相关性差。

关键词: 局灶性脑梗死动物模型, 线栓法, 光化学法, 行为学, 梗死体积

Abstract:

Objective To investigate the two different methods of making animal models of focal cerebral infarction by photochemical method and wire embolization method, and to analyze the correlation between infarct volume and behavior of experimental animals due to different modeling methods. Methods Totally sixty-four male Sprague-Dawley rats of 300-330 g were randomly divided into three groups: normal group, photochemical group and middle cerebral artery occlusion (MCAO) group. The neurological function scores and behavioral tests on the rats were performed 24 hours after surgery. Then brain tissues were removed by anesthesia and execution, and 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed to assess the brain infarct volume in rats. Results There were different volume infarct foci in the brain of SD rats in photochemical group and MCAO group. In MCAO model, most of the hemispheric cortex, striatum, thalamus, hippocampus and subventricular zone were involved in ischemia, and there were good correlations of neurological function scores and balance beam test scores with infarct volumes. In the photochemical model, the infarct foci were in cerebral cortex, and the neurobehavioral abnormalities were well correlated with the cortical infarct location, but the correlation with the infarct volume was relatively poor. Conclusion In MCAO modeling experiment, the behavioral outcomes closely correlated with infarct volume size, while the photochemical model can produce site-specific lesions and site-specific behavioral changed, and the behavioral outcomes have poor correlation with infarct volume.

Key words: Animal model of focal cerebral infarction, Wire embolization method, Photochemical method, Behavior, Infarct volume

中图分类号:

R-332

娄惠娟, 张红石, 王宇峰, 贾萌, 丛德毓. 光化学法与线栓法制作局灶性脑梗死大鼠模型的比较研究[J]. 实验动物与比较医学, 2022, 42(1): 48-53.

Huijuan LOU, Hongshi ZHANG, Yufeng WANG, Meng JIA, Deyu CONG. Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model[J]. Laboratory Animal and Comparative Medicine, 2022, 42(1): 48-53.

图/表 2

图1 各组大鼠的神经功能评分（A）和平衡木行走实验评分（B）注：A组为假手术组，其中A1组除激光照射外，其他处理同B组；A2组实验过程同C组，但不将线栓插入。B组为光化学组，其中B1组为30 mW/mm2照射15 min，B2组为30 mW/mm2照射30 min，B3组为60 mW/mm2照射15 min。C组为线栓法大脑中动脉阻塞（MCAO）组，其中C1组缺血时间为30 min，C2组缺血时间为60 min，C3组缺血时间为120 min，以上3组缺血后再灌注；C4组为永久性梗死，不进行再灌注。*P<0.05，**P<0.01，与C1组相比；#P<0.05，##P<0.01，与C2组相比。

Figure 1 Neurofunctional score （A） and balance beam score （B） of rats in different groups

图2 光化学法和MCAO法建模的各组TTC染色及定量结果注：A组为假手术组，其中A1组除激光照射外，其他处理同B组；A2组实验过程同C组，但不将线栓插入。B组为光化学组，其中B1组为激光强度30 mW/mm2照射15 min，B2组为激光强度30 mW/mm2照射30 min，B3组为激光强度60 mW/mm2照射15 min。C组为线栓法建立大脑中动脉阻塞（MCAO）组，其中C1组缺血时间为30 min，C2组缺血时间为60 min，C3组缺血时间为120 min，以上3组缺血后再灌注；C4组为永久性梗死，不进行再灌注。**P<0.01，与B1组相比；##P<0.01，与B2组相比。△△P<0.01，与C1组相比；▲▲P<0.01，与C2组相比。

Figure 2 TTC staining and quantitative results of each group modeled by photochemistry method and MCAO method

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光化学法与线栓法制作局灶性脑梗死大鼠模型的比较研究

Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model

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