Expression of Smads Protein Family in Radiation-induced Rats Liver Fibrogenesis

doi:10.3969/j.issn.1674-5817.2015.03.003

Abstract

Abstract: Objective To investigate the expression of Smad2/3/7、TGF-β1 proteins in radioactive liver fibrosis process of rats. Methods The experimental animal model of radioactive liver fibrosis was established in male SD rats. The rats were randomly divided into normal control group and irradiated groups (2, 4, 8, 12 and 26 weeks), except normal control group, other groups all were made into model of radioactive liver fibrosis. The rats were sacrificed and isolated liver tissue as backup on 2nd、4th、8th、12th、26th weekend after radiation. The changes of rats liver tissue was detected by HE staining under light microscope. The expression of rats liver tissue Smad2/3/7、TGF-β1 proteins was detected by immunohistochemitry staining. Results Compared with the normal control group, the liver injury and liver fibrosis of irradia ted groups were increased obviously, Smad2 proteins content of liver tissue increased(P<0.05), Smad3、TGF-β1 proteins content increased obviously (P<0.01), and Smad7 proteins content reduced obviously (P<0.01). Conclusion With the aggravation of radioactive liver fibrosis in rats, the expression of Smad2/3、TGF-β1 proteins increased, the expression of Smad7 proteins reduced.

Key words: Smads proteins family, Rats, Radioactive liver fibrosis(RHF)

CLC Number:

Q95-33

GAO Shi-le, HU Zong-tao, QIN Feng, ZHU Jie, HUANG De-wu, DONG Liu-yi. Expression of Smads Protein Family in Radiation-induced Rats Liver Fibrogenesis[J]. Laboratory Animal and Comparative Medicine, 2015, 35(3): 188-194.

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