Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples

doi:10.12300/j.issn.1674-5817.2022.023

Abstract

Abstract:

Objective To observe the effects of serum sample placement time on the detection results of K⁺, Na⁺, Ca²⁺, Cl ^-and pH in sera of SD rats. Methods The sera of 20 male and 20 female SD rats were separated after blood collection from abdominal vein. The samples were covered and stored in a refrigerator at 2-8 ℃. The electrolyte indexes including K⁺, Na⁺, Ca²⁺, Cl^- and pH in serum were measured at 7 time points of 0 h, 2 h, 4 h, 6 h, 24 h, 48 h and 120 h after storage. Results There were no significant differences in the detection results of K⁺ and Na⁺ at each time point (P > 0.05). Compared with the test results at 0 h, the content of Cl^- increased significantly at 48 h and 120 h (P < 0.05); the content of Ca²⁺ decreased significantly at 48 h and 120 hours, and the pH increased significantly at 24 h, 48 h and 120 h (P < 0.05). There was no significant difference in these three indexes within 6 h (P > 0.05). Conclusion The storage time of serum samples of SD rats has a significant impact on the detection results of Ca²⁺, Cl^-and pH in serum. The samples should be covered and stored in a refrigerator at 2-8 ℃ and completed within 6 h after sample preparation.

Key words: Serum, Placement time, K⁺, Na⁺, Ca²⁺, pH, Sprague-Dawley rats

CLC Number:

R-332

Dingshan FENG, Yeyu HUANG, Xiaoxin ZHANG, Aiqin WU, Zhan WANG, Linliang SU. Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples[J]. Laboratory Animal and Comparative Medicine, 2022, 42(4): 301-305.

Figures/Tables 2

References 16

1	樊林花, 李丹, 刘茂林, 等. 清洁级SD大鼠血液生理及生化指标正常参考值的观察[J]. 中国医疗前沿, 2011, 6(19):33-34, 63.
	FAN L H, LI D, LIU M L, et al. Investingation on normal reference range and values of blood physiological and biochemical indexes in clean SD rat[J]. Natl Med Front China, 2011, 6(19):33-34, 63.
2	嘉莉莎. 高血脂对间接离子选择电极法检测电解质的影响[J]. 中国当代医药, 2018, 25(3):148-150. DOI:10.3969/j.issn.1674-4721.2018.03.048 .
	JIA L S. Influence of hyperlipidemia on the detection of electrolyte by indirect ion selective electrode[J]. China Mod Med, 2018, 25(3):148-150. DOI:10.3969/j.issn.1674-4721.2018.03.048 .
3	房玉珠, 姚冬明, 顾红兵, 等. 三种检测系统测定血清电解质的方法学对比与评估[J]. 江苏大学学报(医学版), 2008, 18(1):73-76. DOI:10.13312/j.issn.1671-7783.2008.01.010 .
	FANG Y Z, YAO D M, GU H B, et al. Comparative evaluation of the serum electrolyte determination with three different detection systems[J]. J Jiangsu Univ Med Ed, 2008, 18(1):73-76. DOI:10.13312/j.issn.1671-7783.2008.01.010 .
4	吴芳兰, 应燕萍, 吴卓媚. 中心静脉血与动脉血血浆电解质检测比较的临床研究[J]. 护士进修杂志, 2007, 22(6):494-495. DOI:10.16821/j.cnki.hsjx.2007.06.008 .
	WU F L, YING Y P, WU Z M. Clinical observation on comparing results of plasma electrolyte between blood from central vein and artery[J]. J Nurses Train, 2007, 22(6):494-495. DOI:10.16821/j.cnki.hsjx.2007.06.008 .
5	董树芬. 血样本放置时间和方式对血清电解质测定的影响[J]. 检验医学与临床, 2013, 10(8):1007-1008. DOI:10.3969/j.issn.1672-9455.2013.08.051 .
	DONG S F. Effect of blood sample placement time and method on serum electrolyte determination[J]. Lab Med Clin, 2013, 10(8):1007-1008. DOI:10.3969/j.issn.1672-9455.2013.08.051 .
6	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准 28天经口毒性试验: [S]. 北京: 中国标准出版社, 2015.
	National Health and Family Planning Commission of the People's Republic of China. National food safety standard, 28 day oral toxicity test: [S]. Beijing: China Standards Press, 2015.
7	陈坚, 黄嘉骅, 黄仲义. 血样处理方法对体内电解质钾、钠、氯测定结果评价的影响研究[J]. 中国药房, 2012, 23(46):4401-4403. DOI:10.6039/j.issn.1001-0408.2012.46.31 .
	CHEN J, HUANG J H, HUANG Z Y. Effect of treatment methods of blood sample on determined results evaluation of K⁺, Na⁺ and Cl^- in vivo [J]. China Pharm, 2012, 23(46):4401-4403. DOI:10.6039/j.issn.1001-0408.2012.46.31 .
8	王晓刚, 农乐关, 刘旭, 等. 不同类型外周血标本电解质检测结果比较[J]. 国际检验医学杂志, 2015, 36(10):1356-1357. DOI:10.3969/j.issn.1673-4130.2015.10.015 .
	WANG X G, NONG L G, LIU X, et al. Comparison of electrolyte levels between different peripheral blood samples[J]. Int J Lab Med, 2015, 36(10):1356-1357. DOI:10.3969/j.issn.1673-4130.2015.10.015 .
9	李情操, 穆银玉, 卢雯君, 等. 不同真空采血管和标本放置时间对电解质检测结果的影响[J]. 上海预防医学, 2014, 26(3):149-151. DOI:10.19428/j.cnki.sjpm.2014.03.016 .
	LI Q C, MU Y Y, LU W J, et al. Effect of different placed time for vacutainer and specimen on electrolyte test results[J]. Shanghai J Prev Med, 2014, 26(3):149-151. DOI:10.19428/j.cnki.sjpm.2014.03.016 .
10	吴琰. 不同存储条件对血样电解质及凝血功能指标检测结果的影响[J]. 医疗装备, 2021, 34(9):57-59. DOI:10.3969/j.issn.1002-2376.2021.09.026 .
	WU Y. Influences of different storage conditions on the detection results of blood electrolyte and coagulation function[J]. Med Equip, 2021, 34(9):57-59. DOI:10.3969/j.issn.1002-2376.2021.09.026 .
11	杨利红, 彭小燕, 刘鹏. 样品再定位对电解质分析仪检测结果的影响[J]. 中国城乡企业卫生, 2015, 30(2):115-116. DOI:10.16286/j.1003-5052.2015.02.050 .
	YANG L H, PENG X Y, LIU P. Influence of sample repositioning on the detection results of electrolyte analyzer[J]. Chin J Urban Rural Enterp Hyg, 2015, 30(2):115-116. DOI:10.16286/j.1003-5052.2015.02.050 .
12	钟昆华. 血清电解质钾、钠、氯检测方法比较[J]. 四川解剖学杂志, 2014, 22(4):12-14. DOI:10.3969/j.issn.1005-1457.2014.070 .
	ZHONG K H. Comparison on methods analysis of serum electrolyte of K, Na and Cl[J]. Sichuan J Anat, 2014, 22(4):12-14. DOI:10.3969/j.issn.1005-1457.2014.070 .
13	张耀东. 血清K⁺、Na⁺、Cl^-、HCO₃ ^-检测结果与放置时间相关性的研究[J]. 中外医疗, 2011, 30(3):38, 40. DOI:10.16662/j.cnki.1674-0742.2011.03.050 .
	ZHANG Y D. Study on the correlation between the detection results of serum K⁺, Na⁺, Cl^- and HCO₃ ^- and storage time[J]. China Foreign Med Treat, 2011, 30(3):38, 40. DOI:10.16662/j.cnki.1674-0742.2011.03.050 .
14	晏文强, 邓涛, 刘杨, 等. 血液标本不同保存时间及条件对生化检测结果的影响[J]. 湖北医药学院学报, 2017, 36(4):334-337. DOI:10.13819/j.issn.1006-9674.2017.04.011 .
	YAN W Q, DENG T, LIU Y, et al. The effect of preservation time and condition of blood samples on biochemical tests[J]. J Hubei Univ Med, 2017, 36(4):334-337. DOI:10.13819/j.issn.1006-9674.2017.04.011 .
15	杨惠元. 3种真空采血管不同时间检测电解质的结果比较[J]. 国际检验医学杂志, 2015, 36(19):2893-2894. DOI:10.3969/j.issn.1673-4130.2015.19.053 .
	YANG H Y. Comparison of electrolyte detection results of three kinds of vacuum blood collection tubes at different time[J]. Int J Lab Med, 2015, 36(19):2893-2894. DOI:10.3969/j.issn.1673-4130.2015.19.053 .
16	李红, 敬海明, 林长缨, 等. 大鼠血清葡萄糖测定分析的影响因素[J]. 实验动物与比较医学, 2021, 41(5): 466-468. DOI:10.12300/j.issn.1674-5817.2020.097 .
	LI H, JING H M, LIN C Y, et al. Influence factors of serum glucose measurement and analysis in rats[J]. Lab Animal Comp Med, 2021, 41(5):466-468. DOI:10.12300/j.issn.1674-5817.2020.097 .

实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

[1]	Min LIANG, Yang GUO, Jinjin WANG, Mengyan ZHU, Jun CHI, Yanjuan CHEN, Chengji WANG, Zhilan YU, Ruling SHEN. Construction of Dmd Gene Mutant Mice and Phenotype Verification in Muscle and Immune Systems [J]. Laboratory Animal and Comparative Medicine, 2024, 44(1): 42-51.
[2]	Zhuxin LI, Liang LIANG, Yingying CAO, Shanshan ZHAI, Yinhan DAI, Xia HE, Junyu TAO, Jing LENG, Haibo TANG. Diagnosis of a Primary Pleomorphic Liposarcoma in a Tree Shrew（Tupaia belangeri subsp. yaoshanensis） [J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 647-653.
[3]	Longmei XU, Ruling SHEN, Chun FAN, Wei WU. Generation of 12 Drosophila Transgenic Negative Control Lines Based on Site-specific ΦC31 Integrase and pUASTattB Vector [J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 541-547.
[4]	Liya ZHAO, Liju NI, Caiqin ZHANG, Jianping TANG, Yangzheng YAO, Yanyan NIE, Xiaoxue GU, Ying ZHAO. Establishing a Genetic Detection Protocol of Single Nucleotide Polymorphisms Panels in Inbred Rats Based on Multiplex PCR-LDR [J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 548-558.
[5]	Lingzhi YU, Jianyun XIE, Liping FENG, Xiaofeng WEI. Establishment of Fluorescence qPCR Method for Detection of Staphylococcus Aureus and Its Application in Feces Detection of Rats and Mice [J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 566-573.
[6]	Yasheng DENG, Jiang LIN, Chiling GAN, Guanfeng ZENG, Jiayin HUANG, Huifang DENG, Yingxian MA, Siyin HAN. Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects [J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 406-414.
[7]	Taofeng LU, Hui ZHANG, Jie ZHOU, Qian LI, Shuguang WU, Yanjun WU. Effects of Pogostemon cablin on Serum Metabolomiceof Guizhou Miniature Pigs and It's mechanism [J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 253-261.
[8]	Ziyin XIA, Yuanyuan CHAI, Yunxia XU, Qinwei YU, Xin HUANG, Luyong ZHANG, Zhenzhou JIANG. Quantification of Uric Acid of Rat Serum by Liquid Chromatography-ultraviolet Detection and Its Comparison Study [J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 314-322.
[9]	Ying TAN, Wenping LIAO, Qilong GAO, Yong LI, Xinhui SHI, Jingkun WANG. Physiological Indexes and Histopathology Analysis of Sodium Iodate-Induced Retinitis Pigmentosa in Rats [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 124-135.
[10]	Jingwei MA, Gen LI, Yang YANG, Caixia ZANG, Xiuqi BAO, Dan ZHANG. Comparative Study on Different Recovery Periods of the Spermatogenic Dysfunction Mouse Model Induced by Cyclophosphamide [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 112-123.
[11]	Ling HU, Zhibin HU, Yunqing HU, Yuqiang DING. Overview of Studies in Animal Models of Schizophrenia [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 145-155.
[12]	Huiqing TANG, Shufu CHANG, Zhifeng YU, Lei ZHANG, Xiaoqian TAN, Wei QU, Liang LI, Zhen QIAN, Jianzhong GU, Ping XU. Investigation on Biological Characteristics and Aging Phenotype of SHJH ^hr Mice [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 44-52.
[13]	Ling YANG, Di ZHUANG, Lilun JIN. Screening of Differentially Expressed Genes in Rat Synovitis by Transcriptome Sequencing and in Vitro Verification of Therapeutic Target of Fraxetin [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 11-20.
[14]	Jie WEI, Xinyan ZHANG, Hong WANG, Lan ZHAO, Wei LIU, Huan LI, Rui FU, Han QIAO, Meng ZHAO, Xinhua XIANG, Bingfei YUE. Evaluation Report on the Proficiency Testing of SNP Detection in Laboratory Mice Nucleic Acid Samples [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 505-510.
[15]	Liya YANG, Tao SONG, Jialin HE, Yiming GUO, Mingkang QI, Hanbi WANG, Huiping WANG. Establishment of a Vaginal Atrophy Rat Model and its Application in Pharmacodynamic Evaluation [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 531-540.