The Capacity of Silkworm Cocoon Water to Mitigate the Level of Oxidative Stress in Aged Rats

doi:10.12300/j.issn.1674-5817.2022.027

Abstract

Abstract:

Objective To investigate the capacity of silkworm cocoon water (SCW) to mitigate the level of oxidative stress in aged rats. Methods The samples were processed by simulating the traditional SCW production process, and the SCW components were examined. The 16-month-old rats were randomly divided into 3 experimental groups that were treated with different doses of SCW, and 1 negative control group according to serum malonaldehyde (MDA) content, with 10 rats in each group. Experimental groups were fed SCW at doses of 500, 250, 125 mg/kg body weight, respectively, and the negative control group was given pure water, once a day, for 30 days. At the end of the experiment, the contents of lipid oxidation products (MDA), protein oxidation products (carbonyl), antioxidant enzyme activities (SOD, CAT, GSH-Px), and antioxidant substances (GSH) were measured. Results The crude protein content of SCW was 1 640 mg/100 g, the free amino acid content was 18 mg/100 g, and the hydrolyzed amino acid content was 1 700 mg/100 g. Serine (30.59%), aspartate (18.82%), glycine (10.00%), and threonine (8.24%) were the main hydrolyzed amino acids in SCW. Compared with the negative control group, SCW reduced the contents of MDA and protein carbonyl in the serum and tissue (P < 0.05), but increased the contents of GSH in the serum and tissue, the activity of SOD in tissue, and the activity of CAT and GSH-Px in the serum and tissue of aged rats (P < 0.05). Conclusion SCW has the effect of improving oxidative stress in aged rats.

Key words: Silkworm cocoon water, Sericin, Aged rats, Oxidative stress

CLC Number:

R-332

Huiyan QIN, Huafeng CHEN, Hui YANG, Hailan LUO, Weizhong FU, Qingbo LI, Jiehong ZHANG. The Capacity of Silkworm Cocoon Water to Mitigate the Level of Oxidative Stress in Aged Rats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 393-400.

Figures/Tables 5

Table 1 Amino acid compositions of silkworm cocoon water

氨基酸种类 Amino acid varieties	百分含量 Contents /%
门冬氨酸 Asp	18.82
苏氨酸 Thr	8.24
丝氨酸Ser	30.59
谷氨酸Glu	6.47
脯氨酸Pro	0.82
甘氨酸 Gly	10.00
丙氨酸 Ala	4.18
缬氨酸Val	3.47
异亮氨酸 Ile	0.71
亮氨酸Leu	1.29
酪氨酸 Tyr	4.47
苯丙氨酸 Phe	0.41
赖氨酸 Lys	4.00
组氨酸 His	1.88
精氨酸 Arg	4.65
合计Total	100.00

Table 2 Body weight of rats in each group during the experiment (xˉ±s)

剂量组 Dose group/ (mg·kg^-1)	动物数 Animal numbers	大鼠体质量 Body weight m/g									增质量 Weight gain m/g
剂量组 Dose group/ (mg·kg^-1)	动物数 Animal numbers	Day 1		Day 7		Day 14		Day 21		Day 30	增质量 Weight gain m/g
500	10	854.3±99.1	862.8±100.1		871.4±101.1		878.7±101.6		884.9±102.6		30.6±4.0
250	10	850.3±66.0	859.5±66.7		867.9±57.6		875.8±58.4		883.5±59.0		33.2±4.0
125	10	857.5±122.5	865.9±123.6		873.4±124.5		880.3±125.8		886.3±126.8		28.8±5.0
0	10	861.8±131.8	870.0±132.6		878.3±134.1		885.0±134.5		891.4±135.9		29.6±5.1

Figure 1 Effects of silkworm cocoon water on MDA levels and carbonyl contents in the serum， brain and liver of ratsNote：SCW, silkworm cocoon water. A, serum malonaldehyde (MDA) levels before the experiment; B, serum MDA levels at the end of the experiment; C, brain tissue MDA levels at the end of the experiment; D, hepatic tissue MDA levels at the end of the experiment; E, serum carbonyl contents at the end of the experiment; F, brain tissue carbonyl contents at the end of the experiment; G, hepatic tissue carbonyl contents at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

Figure 2 Effects of silkworm cocoon water on GSH levels and GSH-Px activity in the serum, brain, and liver of ratsNote：SCW, silkworm cocoon water. A, serum reduced glutathione （GSH） levels at the end of the experiment; B, brain tissue GSH levels at the end of the experiment; C, hepatic tissue GSH levels at the end of the experiment; D, serum glutathione peroxidase （GSH-Px） activity at the end of the experiment; E, brain tissue GSH-Px activity at the end of the experiment; F, hepatic tissue GSH-Px activity at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

Figure 3 Effects of silkworm cocoon water on SOD activity and CAT activity in the serum, brain, and liver of ratsNote：SCW, silkworm cocoon water. A, serum superoxide dismutase (SOD) activity at the end of the experiment; B, brain tissue SOD activity at the end of the experiment; C, hepatic tissue SOD activity at the end of the experiment; D, serum catalase (CAT) activity at the end of the experiment; E, brain tissue CAT activity at the end of the experiment; F, hepatic tissue CAT activity at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

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