Table of Content

25 June 2015, Volume 35 Issue 3

Previous Issue    Next Issue

hnRNP U Haploinsufficiency Leads to Growth Retardation, Decreased Activities and Abnormal Glucose Metabolism in Mice

SUN Min, SHA Hai-bo, TU Xin, ZOU Jiang-huan, LAI Bei-bei, GAO Xiang, QI Xin

2015, 35(3):  175-181.  DOI: 10.3969/j.issn.1674-5817.2015.03.001

Asbtract ( 282 )   PDF (858KB) ( 363 )  

References | Related Articles | Metrics

Objective To establish a heterogeneous nuclear ribonucleoprotein U (HnRNP U) knockout mouse model and study the roles of hnRNP U in vivo. Methods The Hnrnpu conventional knockout mouse model was established by homologous recombination. The roles of hnRNP U in vivo were studied by growth analyses, body and tissues weighting, metabolic analyses and glucose tolerance tests. Results The Hnrnpu^-/- mice are embryonic leathal before embryonic day 7.5. The Hnrnpu^+/- mice were partially died at embryonic stage and the viable individuals showed growth retardation with decreased tissues weight, bone mineral density and lean mass compared with wild-type littermates. In addition, hnRNP U haploinsufficiency leads to decreased activity and food intake at night and impaired glucose homeostasis. Conclusion Hnrnpu knockout mouse model was successfully established and hnRNP U played a great role in a diverse group of cellular processes, including the normal growth of somatic tissues, metabolic activities and glucose metabolism.



Establishment of Modified Rat Carotid Endarterectomy Model and Preliminary Observation on Changes of Pathological Morphology

HONG Jing-fang, XU Wei-hua, WANG Shou-sen

2015, 35(3):  182-187.  DOI: 10.3969/j.issn.1674-5817.2015.03.002

Asbtract ( 320 )   PDF (1343KB) ( 337 )  

References | Related Articles | Metrics

Objective To establish a steady and reliable rat model of carotid endarterectomy (CEA) by improved method, and to explore the changes of pathological morphology of vascular wall and proliferation of vascular smooth muscle cells after CEA. Methods Forty-eight SD rats were divided into two groups with 24 rats in each group (modified CEA group and conventional CEA group). The modified model was created by puncturing the carotid artery with a thin needle and detaining sutures before a longitudinal arteriotomy was performed; The success rate of modeling and the operation time were observed to evaluate the characteristics and reliability of two models. HE staining was performed to measure dynamic changes of the thickness of the vascular walls and immunohistochemistry was used to find expression of proliferating cell nuclear antigen (PCNA) on vessel wall on 3 days, 14 days and 28 days after operation. Result The model of rat carotid endarterectomy were successfully constructed. There was no significant difference in the success rate of modeling between two groups (87.5% vs 79.2%, P=0.46). The operation time (31.2±3.7 min) of modified group was significantly lower than that (40.9±4.2 min) of conventional group (P=0.00). Twenty-eight days after the operation, the thickness of the artery and the luminal diameter were significantly different from those of 3 days after the operation in modified group. The positive expression of PCNA was slightly higher on 3 days after operation, the highest on 14 days, and there was no positive expression on 28 days. Conclusion Modified rat carotid endarterectomy model has the characters of high success rate and simple handling, short operation time, and as a steady and reliable model, it is suitable for the study of restenosis after CEA.



Expression of Smads Protein Family in Radiation-induced Rats Liver Fibrogenesis

GAO Shi-le, HU Zong-tao, QIN Feng, ZHU Jie, HUANG De-wu, DONG Liu-yi

2015, 35(3):  188-194.  DOI: 10.3969/j.issn.1674-5817.2015.03.003

Asbtract ( 279 )   PDF (2921KB) ( 362 )  

References | Related Articles | Metrics

Objective To investigate the expression of Smad2/3/7、TGF-β1 proteins in radioactive liver fibrosis process of rats. Methods The experimental animal model of radioactive liver fibrosis was established in male SD rats. The rats were randomly divided into normal control group and irradiated groups (2, 4, 8, 12 and 26 weeks), except normal control group, other groups all were made into model of radioactive liver fibrosis. The rats were sacrificed and isolated liver tissue as backup on 2nd、4th、8th、12th、26th weekend after radiation. The changes of rats liver tissue was detected by HE staining under light microscope. The expression of rats liver tissue Smad2/3/7、TGF-β1 proteins was detected by immunohistochemitry staining. Results Compared with the normal control group, the liver injury and liver fibrosis of irradia ted groups were increased obviously, Smad2 proteins content of liver tissue increased(P<0.05), Smad3、TGF-β1 proteins content increased obviously (P<0.01), and Smad7 proteins content reduced obviously (P<0.01). Conclusion With the aggravation of radioactive liver fibrosis in rats, the expression of Smad2/3、TGF-β1 proteins increased, the expression of Smad7 proteins reduced.



Construction of Recombinant Duck Enteritis Virus with HN Gene of Newcastle Disease Virus

LIANG Shu-lin, LI Hui-xin, CHEN Hong-yan, LIU Sheng-wang

2015, 35(3):  195-201.  DOI: 10.3969/j.issn.1674-5817.2015.03.004

Asbtract ( 225 )   PDF (1712KB) ( 357 )  

References | Related Articles | Metrics

Objective To construct the recombinant duck enteritis virus (DEV) expressing the HN gene of Newcastle disease virus (NDV). Methods The HN gene of NDV was inserted into TK gene of DEV Clone-03 by homologous recombination technology. rDEV TK-EGFP which was constructed as parent virus, by means of cotransfection of the viral genome and transfer vector and plaque purification, the recombinant duck enteritis virus was obtained expression the HN gene of Newcastle disease virus. Results The HN gene of NDV was inserted into DEV genome correctly and expressed effectively on gene and protein level. Replication kinetics and the genetic stability detection of the recombinant virus rDEV TK-HN proved that the titer of rDEV TK-HN decreased slightly, but the trend was consistent with the parent virus replication. After 20 passages in chicken embryo fibroblast and SPF chicken embryos, it showed that the HN gene was of good genetic and expression stability with the passage of the virus. Conclusion By homologous recombination technology and modifying duck enteritis virus genome, the recombinant virus which could carry the HN gene of NDV was constructed and the virus was of good genetic and expression stability.



Effects of Exercise on Food Intake and Body Composition in Obesity-prone and Obesity-resistant Rats

LI Jing-jing, ZHANG Yun-li, CAI Ming, XIN Lei, LOU Shu-jie

2015, 35(3):  202-206.  DOI: 10.3969/j.issn.1674-5817.2015.03.005

Asbtract ( 229 )   PDF (530KB) ( 311 )  

References | Related Articles | Metrics

Objective To observe the effects of exercise on food intake and body composition in obesity-prone and obesity-resistant rats, in order to provide experimental evidence for fitness and exercise for weight loss. Methods Seventy male SD rats were fed with high-fat diet or normal diet, after 8 weeks, obesity-prone and obesity-resistant rats were screened out based on body weight; then they were divided into four groups: obesity-prone quiet group (OP), obesity-prone exercise group (OPE), obesity-resistant quiet group (OR) and obesity-resistant exercise group (ORE), and all animals were continued to be given high-fat diet, the exercise groups were arranged to adaptive exercise for one week, then were performed eight weeks treadmill exercise, and the body composition of rats were measured using the Prodigy dual-energy X-ray absorptiometry. Results (1) Compared with the quiet groups, food intake in the exercise groups were decreased significantly; (2) Compared with the quiet groups, body weight and fat tissue in the exercise groups were significantly decreased, the amount of muscle tissue of rats in OPE group were significantly increased, and the amount of muscle tissue of rats in ORE group were not changed significantly. Conclusion The same intensity exercise can produce different effects on the amount of muscle tissue in obesity-prone and obesity-resistant rats, it is necessary to fully take the intensity and nutritional supplements into account when exercise used for fitness.



Evaluation on Effects of Social Contact Test in Predicting Training Successful Rate for Guide Dogs

ZHOU Zi-juan, ZHANG Ya-li, HAN Fang, YU Jian-xiong, WANG Liang, JIANG Xin, WANG Ai-guo, WANG Fu-jin, WANG Jing-yu

2015, 35(3):  207-211.  DOI: 10.3969/j.issn.1674-5817.2015.03.006

Asbtract ( 314 )   PDF (466KB) ( 296 )  

References | Related Articles | Metrics

Objective To establish the methods and evaluation criterion for social contact test and detect their effects on predicting the training successful rate for guide dogs. Method By improving the method of dog mentality assessment (DMA), the methods of social contact test and the corresponding grading standards in detail were established. The 17 guide dogs and 17 eliminated dogs from Chinese Guide Dogs Training Centre of Dalian were used to examine and evaluate the predicting effects of the 3 behavioral variables in social contact test (greeting reaction, cooperation, and handling) on the training successful rate for guide dogs. Results The scores of greeting reaction variable did not show any significant difference between guide dogs and eliminated dogs (guide dogs: x- =3.29, eliminated dogs: x- =3.18, P=0.716). While the scores of cooperation and handling variables of guide dogs were significantly higher than those of eliminated dogs (cooperation: guide dogs: x- =4.29, eliminated dog: x- =3.41, P=0.005; handling: guide dogs: x- =3.59, eliminated dogs: x- =3.00, P=0.049). The breed and gender of dogs in social contact test had no significant effect on the scores of the behavior variables (P>0.05). Conclusion The method and evaluation criterion of social contact test was successfully established, and the cooperation and handling can be used as effective behavior variables to predict the training successful rate for guide dogs.



Evaluation on Effects of Play and Passive Situation Tests in Predicting Training Successful Rate for Guide Dogs

ZHANG Ya-li, ZHOU Zi-juan, YU Jian-xiong, HAN Fang, WANG Liang, JIANG Xin, AI Bo, WANG Ai-guo, WANG Fu-jin, WANG Jing-yu

2015, 35(3):  212-217.  DOI: 10.3969/j.issn.1674-5817.2015.03.007

Asbtract ( 245 )   PDF (684KB) ( 307 )  

References | Related Articles | Metrics

Objective To establish the methods and evaluation criterion for play and passive situation tests of DMA (dog mentality assessment) and detect their effects on predicting the training successful rate for guide dogs. Method According to the methods of common temperament tests and considering the behavior characteristics of guide dogs, the methods of play and passive situation tests for guide dogs’ behavior and the related evaluation criterion for 5 behavioral variables in total were established. The behavioral variables were Interest in play, grabbing, tug-of-war in play test and activity I and activity II in passive situation test. Seventeen guide dogs and 17 eliminated dogs from Chinese Guide Dogs Training Centre of Dalian were used to examine the predicting effects of the 5 behavioral variables on the training successful rate for guide dogs. Result According to the results of statistical analysis, the “activity I” in the passive situation test showed higher significant difference between guide dogs and eliminated dogs (P=0.003), and the “activity II” in the passive situation test showed significant difference (P=0.014). However, no significant difference was detected for the rest variables in play test between guide dogs and eliminated dogs (P>0.05). While, there were clear trends showed that the scores of eliminated dogs were higher than that of guide dogs in variable of Interest in play. The distribution analysis of the numbers of guide dogs and eliminated dogs in 3 different score grades (1~2 points, 3~4 points and 5 points) of each variable showed: for Interest in play, more guide dogs distributed in 1~2 points and less guide dogs distributed in 5 points compared to eliminated dogs; for grabbing, the distribution of guide dogs and eliminated dogs had a similar trend; for tug-of-war, more eliminated dogs distributed in 1~2 points and 5 points grade compared to guide dogs; for activity I and activity II, more guide dogs distributed in 1~2 points and less guide dogs distributed in 3~4 points compared to eliminated dogs. Conclusion The methods and evaluation criterion for play and passive situation test were successfully established. The “activity I” and “activity II” are confirmed as two effective variable indexes to predict the training successful rate for guide dogs.



Application of PCR and Restiction Enzyme in Genotype Identification of uPA-SCID Mouse

XU Chun-hua, CHEN Li-xiang, REN Xiao-nan, YANG Yu-qin, PENG Xiu-hua, CAI Jia-lin, ZHOU Xiao-hui, ZHOU Wen-jiang

2015, 35(3):  218-221.  DOI: 10.3969/j.issn.1674-5817.2015.03.008

Asbtract ( 270 )   PDF (710KB) ( 297 )  

References | Related Articles | Metrics

Objective To set up a simple, fast and accurate method for the genotype identification of uPA-SCID mouse . Method The genotype of uPA-SCID mouse was detected by using PCR combined with restriction enzyme. Result The genotype identification results of uPA showed that the stripes of wild type in 153 bp, the stripes of homozygous in 337 bp, and the stripes of heterozygote in 337 bp and 153 bp. The genotype identification results of SCID showed that the mutant genes stripes in 38 bp and 26 bp, the heterozygote genes stripes in 64 bp, 38 bp and 26 bp, and the wild type stripes in 64 bp. Conclusion The method established is simple and accurate, it can identify the genotypes of uPA-SCID mouse, and screening the needful homozygous uPA-SCID mouse.



Study on Changes of Body Temperature in Japanese White Rabbit and New Zealand Rabbit under Physiological Conditions

WANG Feng, HAO Fei, HUAN Hong-hua, ZHANG Yuan-peng, ZHANG Bo, SHAO Guo-qing

2015, 35(3):  222-226.  DOI: 10.3969/j.issn.1674-5817.2015.03.009

Asbtract ( 289 )   PDF (673KB) ( 306 )  

References | Related Articles | Metrics

Objective To investigate the change rules of the body temperature in conventional Japanese white rabbits and New Zealand rabbits under physiological condition. Methods In the environment of 23.5-28 ℃ and relative humidity 54%-65%, the body temperature of rabbits in free stateus were determined continuously by using TOSHONG wireless automatic temperature measurement platform. The 12 480 points of rabbits’ temperature picked from 312 000 times of measurements were analysed. Results Feeding the rabbits quantitatively in the same time in morning and evening, the tested animals’ circadian body temperature is the W type. 1∶00-6∶00, 15∶00-18∶00 are the rise period of temperature, 7∶00-11∶00, 20∶00-0∶00 are the descend period of temperature, 5∶00-8∶00, 18∶00-20∶00 are the peak of temperature, 23∶00-2∶00, 11∶00-15∶00 are the trough of temperature. The rabbits’ body temperature changes bigger than usual in the next 5 hours after they eating. Conclusions The rabbits’ body temperature showed downtrend in the next 2-5 hours after they eating, 23∶00-2∶00, 11∶00-15∶00 are the trough of temperature. Pyrogen test is suitable for to taken in 0∶00-3∶00 and 12∶00-15∶00. The method which widely used in animal experiments according to the method of average 24 hours time interval measurement may be remained consultative.



Application of Problem-based Learning with Case-based Teaching for Junior College Students in Laboratory Animal Science Experiment

QIAO Wei-wei, ZHAO Xian-zhe

2015, 35(3):  245-248.  DOI: 10.3969/j.issn.1674-5817.2015.03.015

Asbtract ( 191 )   PDF (376KB) ( 275 )  

References | Related Articles | Metrics

Objective To explore the effects of problem-based learning (PBL) with case-based teaching in experiment of laboratory animal science. Methods Sixty experiment objects randomly selected every year from junior college students during 2012-2014 were divided into two groups. In class A, lecture-based learning (LBL) teaching mode was used. In class B PBL with case-based teaching mode was used. And then the teaching effect was evaluated by means of the questionnaire survey and experiment report grading. Results The students of class B were superior to that of class A on how to design the animal experiment, experimental animal ethics practical applications, analytical ability of animal experiment results, animal experiment report writing, who showed good teaching effect. Conclusion The application of PBL with case-based teaching mode in laboratory animal science experiment could increase their learning enthusiasm by stimulating interests of students and improvement of the quality of experimental teaching.

Advances on Research of APOE Gene Function and Related Diseases Models

JIANG Wei-hua, LI Shan-gang, CHEN Xue-jin

2015, 35(3):  249-257.  DOI: 10.3969/j.issn.1674-5817.2015.03.016

Asbtract ( 347 )   PDF (554KB) ( 324 )  

References | Related Articles | Metrics

Apolipoprotein E gene (APOE) is located on human chromosome 19, with 3.7 kb length. Its expression production is an alkaline protein containing arginine. ApoE can take part in lipid transport, storage and excretion, as well as nervous system development and injury repair process. High incidence of atherosclerosis and Alzheimer’s disease occurred in human are related with lipid metabolism dysregulation. Researches on animals in vitro showed that the polymorphism of ApoE is related to coronary heart disease, hyperlipidemia and infarction. Transgenic and gene knock out or mutation animal models can help to know more about the process of lipid metabolism, understand the mechanism of occurrence and development process , and find the potential therapeutic targets of related illnesses. Then more effective solutions for the treatment of human diseases can be provided. This essay briefly summaries the mechanism of some diseases related to APOE genotype and apoE protein, and also researches on the development of these diseases in vitro in animal models.

The Application of Metabonomics in Genetic Quality Monitoring of Spontaneous Disease Animal Models and Development of Specialized Feed

WANG Long-xia, ZHAO Xian-zhe, QIAO Wei-wei

2015, 35(3):  258-263.  DOI: 10.3969/j.issn.1674-5817.2015.03.017

Asbtract ( 197 )   PDF (557KB) ( 296 )  

References | Related Articles | Metrics

Hereditary spontaneous disease animal models are powerful tools for the study of diseases like diabetes mellitus, hypertension and obesity etc. However, these special animal models are prone to be influenced by environment and diets. In this review, the metabonomics, a new research tool, to be used in discovering the biomarkers of these special animal models were introduced. The practicability of using these biomarkers to monitor the genetic quality of these special animal models and the necessity of developing the appropriate feeds for them were explained, so as to provide some references for future research.