Abstract: Objective To compare several frequently used surrogate indexes of insulin sensitivity and the euglycemic-hyperinsulinemic clamp experiments in high-fat diet rats. Methods Thirty male Wistar rats were randomly divided into two groups with a regular (NC group, n=15) or high-fat diet (HF group, n=15) for 10 weeks.The euglycemic-hyperinsulinemic clamp experiments,the insulin tolerance tests as well as the glucose tolerance tests were performed and the correlations of the euglycemic-hyperinsulinemic clamp experiments with different types of IR indexes were conducted. Result The glucose infusion rate [GIR (0.90±0.15 vs 4.97±0.68)mg·kg^-1 ·min^-1, P<0.01and KITT (24.41±6.77 vs 48.61±4.71, P<0.01) in HF group decreased significantly. The insulin tolerance tests correlated with the euglycemic-hyperinsulinemic clamp experiments significantly (r=0.911, P<0.01). Conclusion This study demonstrates that the insulin tolerance tests correlated with the euglycemic-hyperinsulinemic clamp experiments significantly.

Key words: Insulin sensitivity, High-fat diet, Rats