The Effect of Six Kinds of Traditional Chinese Herbal Composing Prescriptions on Cell Proliferation and Melanogenesis in Cultured B16 Melanoma Cells

doi:10.3969/j.issn.1674-5817.2011.01.011

Abstract

Abstract: Objective To study the effects of six ethanol extracts and aqueous extracts from traditional Chinese herbal composing prescriptions on the cell proliferation and melanogenesis in cultured B16 melanoma cells. Methods The cell proliferation were measured by MTT method, and NaOH assay was to determine melanin synthesis. Results The aqueous extracts of composition 1,2,3 and alcohol extracts of composition 2,4,6 had significantly promoted cell proliferation (P<0.05); aqueous extract with the highest rate of cell proliferation was composing prescription 2 (0.625 mg/ml), the proliferation rate was 22.60%; ethanol extract with the highest rate of cell proliferation was composition 4 (0.078 mg/ml), the proliferation rate was 11.60%. The aqueous extract of composing prescriptions 1,2,4,5,6 and alcohol extracts of the six kinds of composing prescriptions promoted obviously the synthesis of melanin (P<0.05). Aqueous extract with the highest rate of melanin synthesis was composing prescription 1 (0.313 mg/ml), the growth rate is 75.38%; alcohol extract with the highest rate of melanin synthesis was composing prescription 1 (1.25 mg/ml), the growth rate was 88.01%. Conclusion The positive effect of each composing prescription on the proliferation of mice’s B16 melanoma cells and melanin synthesis is various due to the composing of the prescription.

Key words: Composing prescriptions, B16 melanoma cells, Cells proliferation, Melanogenesis

CLC Number:

Q95-33

ZHANG Jian,WU Wei,WAN Yu-hua,et al. The Effect of Six Kinds of Traditional Chinese Herbal Composing Prescriptions on Cell Proliferation and Melanogenesis in Cultured B16 Melanoma Cells[J]. Laboratory Animal and Comparative Medicine, 2011, 31(1): 52-56. DOI: 10.3969/j.issn.1674-5817.2011.01.011.

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