Changes and Significance of Arterial Blood Gas Analysis Indexes in Rats with Chronic Hypoxic Pulmonary Hypertension

doi:10.12300/j.issn.1674-5817.2020.080

Abstract

Abstract: Objective To investigate the changes and significance of arterial blood gas analysis indexes in a rat model of pulmonary hypertension induced by chronic hypoxia. Methods Thirty-six SD male rats were randomly divided into the normoxic control group and the hypoxic groups. The rats in the hypoxic groups were placed in a hypoxic chamber (10% oxygen concentration) for 1, 3, 7, 14, 21 days, respectively. Right ventricular systolic pressure (RVSP) was measured by right ventricular catheter, and right ventricular hypertrophy index (RVHI) was calculated by calculating the weight ratio of right ventricle (RV) to [left ventricle (LV) + ventricular septum (S)]. Masson staining was used to observe the pathological changes of pulmonary artery. Blood was taken from the abdominal aorta for the arterial blood gas analysis to determine the following indexes: pondus hydrogenii (pH), arterial partial pressure of carbon dioxide (PaCO₂), arterial partial pressure of oxygen (PaO₂), arterial oxygen saturation (SaO₂), bicarbonate (HCO₃^–), sodium (Na⁺), potassium (K⁺), calcium (Ca²⁺), hematocrit (Hct) and hemoglobin (Hb). Results RVSP and RVHI of the rats were significantly increased in the hypoxic groups compared with the normoxic control group (P<0.05). Compared with the normoxic control group, the hypoxic groups (7, 14, 21 days) had abnormal pulmonary artery remodeling. Compared with the normoxic control group, the pH and HCO₃^– levels of the hypoxic groups were significantly reduced (P<0.05); PaCO₂ level was significantly increased in the hypoxic 21-day group (P<0.05), PaO₂ and SaO₂ levels were significantly decreased in the hypoxic 14-day group (P<0.05), the Na⁺ concentration (except hypoxia 1-day group) and the Ca²⁺ concentration (hypoxic 3-day group) were significantly reduced (P<0.05); the K⁺concentrations were increased significantly in the hypoxic groups (except hypoxia 14-day group) (P<0.05); Hct and Hb were significantly increased in the hypoxic groups (P<0.05). There was a significant negative correlation between Na⁺ and RVSP in rats with hypoxic pulmonary hypertension (P<0.05). Conclusion The arterial blood gas analysis indexes, including pH, PaCO₂, PaO₂, SaO₂, HCO₃^–, Na⁺, K⁺, Ca²⁺, Hct and Hb are significantly changed, and participate in and regulate the development and progression of hypoxic pulmonary hypertension in rats, in which Na⁺ level may be used as an indicator to evaluate the severity of hypoxic pulmonary hypertension indirectly.

Key words: Chronic hypoxia, Pulmonary hypertension, Vascular remodeling, Blood gas analysis, Rats

CLC Number:

Q95-33

ZHANG Shuting, YAO Qingqing, LI Yishan, SHI Yiwei. Changes and Significance of Arterial Blood Gas Analysis Indexes in Rats with Chronic Hypoxic Pulmonary Hypertension[J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 27-32.

Figures/Tables 6

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