Establishment of Ulcerative Colitis Model in Mice by Different Drug Delivery Methods of Dextran Sodium Sulfate

doi:10.12300/j.issn.1674-5817.2020.090

Abstract

Abstract: Objective To study the modeling situation of ulcerative colitis in mice by gavage or free drinking of dextran sulfate sodium (DSS) respectively. Methods Thirty two male C57BL/6 mice were randomly divided into a blank control group, a 3% DSS free drinking group, 5 g/kg and 6 g/kg DSS gavage groups, with eight mice in each group. The drug treatments lasted for 7 days. The water intake, body weight, stool characteristics and hematochezia of mice in each group were recorded daily, and the disease activity index (DAI) score of mice was calculated daily. After the mice were sacrificed on the 8th day, the colon, thymus and spleen were separated, the thymus and spleen were weighed, and the immune organ index was calculated; the colon was measured for length and observed for pathological changes, and the histopathological score was assessed. Results The total intake of DSS in each administration group exceeded 30 mg/g for 7 days. Compared with the blank control group, there was no significant difference in the DAI score of mice in the gavage groups (P>0.05), and the DAI score of mice in the free drinking group increased significantly from the 3rd day (P<0.01). Compared with the blank control group, the colon of mice in each administration group was shortened (P<0.01), but the thymus index showed no significant difference (P>0.05). The decreasing of thymus index and colon atrophy of mice in the free drinking group were more significant than those in the gavage groups (P<0.05). In terms of histopathological changes, the mice in the 6 g/kg gavage group had slight pathological changes, and the mice in the free drinking group had more significant pathological changes, and the histopathological score in the free drinking group was significantly higher than those in the gavage groups (P<0.01 or P<0.05). Conclusion When DSS is used to establish the model of ulcerative colitis in mice, the free drinking method is more effective than the gavage administration.

Key words: Ulcerative colitis, Dextran sodium sulfate, Free drinking, Gavage, Mice

CLC Number:

Q95-33

ZHAO Yani, LI Yao, ZHANG Yan, WANG Siwang, AN Junming. Establishment of Ulcerative Colitis Model in Mice by Different Drug Delivery Methods of Dextran Sodium Sulfate[J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 33-39.

Figures/Tables 5

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