关键词: FSHR, 主动免疫, 卵巢早衰模型, SD大鼠

Abstract: Objective To establish the rat model of premature ovarian failure by immune suppression to FSH receptor (FSHR), and to evaluate their phenotype. Methods On the basis of biological information of the whole FSHR protein structure, the extracellular domain of FSHR was cloned. The recombinant protein obtained by prokaryotic expression and three synthetic peptides of the protein were used to immunize 4 weeks old female SD rats. Estrous cycle, and blood FSH, estrogen and progesterone were detected separectely. Results After the active immunization, high titer antibodies were detected in the blood and lasted for 6 weeks. The antibodies were shown to specifically bind with the granulosa cells in vitro and at the same time, the ovarian response to gonadotropins was reduced in vivo. Estrous cycle disorders with shortened estrus, blood FSH rise and estrogen decrease were detected in rats immunized with recombinant protein; and the litter size was decreased while mating with normal males. Conclusion The active immunization in rats against FSHR ligand binding sites resulted in high titers of FSHR antibodies in the blood, which could specifically bind to ovarian granulosa cells, and resulted in lower response to gonadotropin in ovaries, and then symptoms of premature ovarian failure were observed in rats.

Key words: FSHR, Active immunization, Premature ovarian failure model, SD rats