葡聚糖硫酸钠不同给药方式建立小鼠溃疡性结肠炎模型

doi:10.12300/j.issn.1674-5817.2020.090

摘要/Abstract

摘要： 目的研究小鼠分别经灌胃或自由饮用葡聚糖硫酸钠（dextran sodium sulfate,DSS）后发生溃疡性结肠炎的成模情况。方法选取32只雄性C57BL/6小鼠,随机分为空白组、3% DSS自由饮用组、5 g/kg和6 g/kg DSS灌胃组,每组8只,连续给药7 d。每天记录各组小鼠的饮水量、体质量、粪便性状及便血情况,进行小鼠疾病活动指数（disease activity index,DAI）评分。第8天处死小鼠,分离小鼠结肠、胸腺及脾脏,称胸腺和脾脏质量,计算免疫器官指数;测量结肠长度,观察结肠病理变化,进行组织病理学评分。结果各给药组小鼠7 d的DSS总摄入量均超过30 mg/g。与空白组比较,灌胃组小鼠的DAI评分无明显差异（P>0.05）,自由饮用组小鼠从第3天起DAI评分明显升高（P<0.01）。与空白组比较,各给药组小鼠的结肠明显缩短（P<0.01）,但胸腺指数差异无统计学意义（P>0.05）;自由饮用组小鼠的胸腺指数下降和结肠萎缩程度较灌胃组更明显（P<0.05）。组织病理学改变方面,6 g/kg灌胃组小鼠结肠发生轻微病理学变化;自由饮用组小鼠结肠的病理学改变更为显著,组织病理学评分明显高于灌胃各剂量组（P<0.01或P<0.05）。结论采用DSS建立小鼠溃疡性结肠炎模型时,自由饮用的方式较灌胃造模效果更好。

关键词: 溃疡性结肠炎, 葡聚糖硫酸钠, 自由饮用, 灌胃, 小鼠

Abstract: Objective To study the modeling situation of ulcerative colitis in mice by gavage or free drinking of dextran sulfate sodium (DSS) respectively. Methods Thirty two male C57BL/6 mice were randomly divided into a blank control group, a 3% DSS free drinking group, 5 g/kg and 6 g/kg DSS gavage groups, with eight mice in each group. The drug treatments lasted for 7 days. The water intake, body weight, stool characteristics and hematochezia of mice in each group were recorded daily, and the disease activity index (DAI) score of mice was calculated daily. After the mice were sacrificed on the 8th day, the colon, thymus and spleen were separated, the thymus and spleen were weighed, and the immune organ index was calculated; the colon was measured for length and observed for pathological changes, and the histopathological score was assessed. Results The total intake of DSS in each administration group exceeded 30 mg/g for 7 days. Compared with the blank control group, there was no significant difference in the DAI score of mice in the gavage groups (P>0.05), and the DAI score of mice in the free drinking group increased significantly from the 3rd day (P<0.01). Compared with the blank control group, the colon of mice in each administration group was shortened (P<0.01), but the thymus index showed no significant difference (P>0.05). The decreasing of thymus index and colon atrophy of mice in the free drinking group were more significant than those in the gavage groups (P<0.05). In terms of histopathological changes, the mice in the 6 g/kg gavage group had slight pathological changes, and the mice in the free drinking group had more significant pathological changes, and the histopathological score in the free drinking group was significantly higher than those in the gavage groups (P<0.01 or P<0.05). Conclusion When DSS is used to establish the model of ulcerative colitis in mice, the free drinking method is more effective than the gavage administration.

Key words: Ulcerative colitis, Dextran sodium sulfate, Free drinking, Gavage, Mice

中图分类号:

Q95-33

赵亚妮, 李瑶, 张妍, 王四旺, 安军明. 葡聚糖硫酸钠不同给药方式建立小鼠溃疡性结肠炎模型[J]. 实验动物与比较医学, 2021, 41(1): 33-39.

ZHAO Yani, LI Yao, ZHANG Yan, WANG Siwang, AN Junming. Establishment of Ulcerative Colitis Model in Mice by Different Drug Delivery Methods of Dextran Sodium Sulfate[J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 33-39.

图/表 5

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