›› 2006, Vol. 26 ›› Issue (3): 131-133.

• 论文 •    下一篇

阿米诺利敏感的小鼠生精细胞离子电流

  

  1. 1.南京医科大学应用毒理省重点实验室,南京 210029;2.江苏省疾病控制中心,南京 210009
  • 收稿日期:2006-05-31 出版日期:2006-09-25 发布日期:2006-09-25
  • 基金资助:

    国家自然科学基金资助项目(30571555),国家973基础研究重大资助项目(2002CB512908)

Currents Sensitive to Amiloride in Mouse Spermatogenic Cells

  1. 1.Institute of Applied Toxicology of Nanjing Medical University, Nangjing 210029, China;2.Center of Disease Control and Prevention of Jiangsu Province, Nanjing,210009, China
  • Received:2006-05-31 Online:2006-09-25 Published:2006-09-25

摘要: 目的 研究记录小鼠生精细胞上表皮钠通道电流。方法 应用机械分离获得小鼠单个生精细胞,采用全细胞膜片钳技术记录上皮钠离子通道电流。结果 当钳制电位在-50mV、指令电压-100~+40mV、步阶电压10mV,测试脉冲为200ms时,可记录到一快速激活并且不失活的电流。细胞外液中加入1μmol/L的阿米诺利能显著性抑制该电流,并呈现出两类不同的作用(抑制率分别为58.49±9.82%,31.44±5.69%)。结论 电流特性和阿米诺利敏感性特征表明,生精细胞上所记录到的电流是上皮钠通道电流。

关键词: 生精细胞, 全细胞膜片钳技术, 上皮钠通道, 小鼠

Abstract: Objective To observe epithelial sodium channels (ENaC) currents in spermatogenic cells. Method Epithelial sodium channels were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp. Results The currents were evoked by 200 ms test pulses between-100?+40 mV in 10 mV increments from a holding potential (HP) of -50 mV. Test pulses elicited rapidly activating and non-inactivating Na+ current. Na+ currents amplitude were significantly reduced by adding Amiloride (1 μmol/L) to the external solution, and displayed two kinds of inhibition effects (the ratio of 58.49%±9.82%,31.44%±5.69%). Conclusion The characteristics of such currents and sensitive to Amiloride all approved that these currents recorded from mouse spermatogenic cells were produced through epithelial sodium channels.

Key words: Spermatogenic cells, Whole-cell patch clamp, Epithelial sodium channels, Mouse