实验动物与比较医学 ›› 2024, Vol. 44 ›› Issue (5): 502-510.DOI: 10.12300/j.issn.1674-5817.2024.034

• 实验动物福利与伦理 • 上一篇    下一篇

不同发育时期小鼠代谢笼实验的最佳适应期探讨

谭贺(), 杨晓辉, 张大秀, 王贵成()()   

  1. 复旦大学发育生物学研究所, 上海 200433
  • 收稿日期:2024-02-29 修回日期:2024-06-05 出版日期:2024-11-06 发布日期:2024-10-25
  • 通讯作者: 王贵成(1985—),男,硕士,工程师,研究方向:实验动物平台建设。E-mail: wangguicheng@fudan.edu.cn。ORCID: 0000-0002-3979-419X
  • 作者简介:谭 贺(1980—),女,硕士,实验师,研究方向:实验动物表型分析。E-mail: tanhe@fudan.edu.cn

Optimal Adaptation Period for Metabolic Cage Experiments in Mice at Different Developmental Stages

TAN He(), YANG Xiaohui, ZHANG Daxiu, WANG Guicheng()()   

  1. Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China
  • Received:2024-02-29 Revised:2024-06-05 Published:2024-10-25 Online:2024-11-06
  • Contact: WANG Guicheng (ORCID: 0000-0002-3979-419X), E-mail: wangguicheng@fudan.edu.cn

摘要:

目的 探究小鼠代谢笼实验中不同发育时期小鼠的最佳适应时间,以期为利用小鼠开展代谢研究提供参考。 方法 使用3个发育阶段(离乳期M1、青春期M2和成年期M3)的雄性C57BL/6J小鼠共80只进行为期7 d的代谢笼实验,每5 min记录小鼠摄食、饮水、能量消耗、呼吸商、体重和活动水平等数据。通过时间序列分解和综合聚类分析对数据进行处理,采用重复测量方差分析法并结合t检验进行统计学差异比较,以推断最佳适应时间。 结果 不同发育阶段的小鼠代谢活动存在明显差异(P<0.01)。与M2和M3小鼠相比,M1小鼠的运动水平较低(P<0.01),并且昼夜节律不明显。M1小鼠具有更高的氧气消耗量、二氧化碳呼出量和能量消耗水平,以及较低的呼吸商(均P<0.001),表明其主要以脂肪作为能量来源。对摄食、饮水和能量消耗等因素进行分析后发现,各发育阶段的小鼠在第1个光周期(0~12 h)与 第2个光周期之间(24~36 h)存在明显差异(均P<0.05),而24 h后每日摄食量、饮水量不再有明显差异(均P>0.05)。综合多指标进行聚类分析后发现,小鼠在进入代谢笼的前24 h综合指标与后续6 d的综合指标不成簇,出现离群,表现出明显差异。 结论 小鼠代谢笼实验可以用于检测小鼠连续的生理学指标变化,结果提示小鼠进入代谢笼24 h就可以适应新的代谢笼环境,这为小鼠代谢实验设计提供了理论依据。

关键词: 代谢笼, 适应期, 摄食, 饮水, 聚类分析, 小鼠

Abstract:

Objective To investigate the optimal adaptation period for mice at different developmental stages during metabolic cage experiments, aiming to provide a reference for conducting metabolic research using mice. Methods A total of 80 male C57BL/6J mice at three developmental stages (weaning period M1, adolescent M2, and adulthood M3) were subjected to a 7-day metabolic cage experiment. Data on food intake, water intake, energy expenditure, respiratory quotient, body weight, and activity levels were recorded every five minutes. The collected data were processed using time series decomposition and comprehensive cluster analysis. Statistical differences were compared using repeated measures ANOVA combined with t-test to determine the optimal adaptation period. Results Significant differences in metabolism were observed among mice in different developmental stages (P<0.01). Compared with adolescent (M2) and adult (M3) mice, weaned mice (M1) exhibited lower activity level (P<0.01) and less distinct circadian rhythm. M1 mice had higher oxygen consumption, carbon dioxide production, and energy expenditure, as well as a lower respiratory quotient (all P<0.001), indicating that they mainly relied on fat as an energy source. Analysis of food intake, water intake, and energy expenditure revealed significant differences between the first light cycle (0-12 h) and the second light cycle (24-36 h) across all developmental stages (all P<0.05) . However, there was no significant difference in daily food intake or water intake after 24 hours (both P>0.05). Comprehensive cluster analysis of multiple indicators showed that the overall indicators of mice during the first 24 hours in the metabolic cages did not cluster with those of the subsequent 6 days, demonstrating significant differences. Conclusion Metabolic cage experiment can be used to detect continuous physiological changes in mice. The results suggest that mice can adapt to new metabolic cages environment within 24 hours, providing a theoretical basis for the design of metabolic experiments using mice.

Key words: Metabolic cage, Adaptation period, Food intake, Water intake, Cluster analysis, Mice

中图分类号: