关键词: 小鼠腺病毒, 聚合酶链式反应, 长爪沙鼠, 小鼠

Abstract: Objective To develop a RT-PCR method for detection of Mouse Adenovirus (MAdV) in Mongolian gerbils and Laboratory mice. Methods The primers were designed and synthesized according to the published MAdv specific sequences of E1B gene . PCR method is established, and the specificity, sensitivity, stability test were carried out. The method is used to detect 65 Mongolian gerbils, and 12 mice. Results The developed PCR method was no cross reaction with Minute virus, Polyoma virus, its minimum detection limit ,using the recombinant plasmid containing MAdV gene as a template, was 1.67 pg/μl, and the lowest detection virus titer is 10^-7/ml. The MAdV cDNA maintained at -30℃ for 12 months, still can enlarge the size of about 606 bp visible mesh belt. The 65 Mongolian gerbils and 12 mice were all negative about MAdv detected by PCR. Conclusion The developed PCR method is good in specificity, sensitivity, stability,and may be used for detecting the MAdv in laboratory animal, such as Mongolian gerbils and mice.

Key words: Mouse Adenovirus, PCR, Mongolian gerbil, Mouse