三个封闭群实验兔线粒体DNA D-loop区多态性分析

doi:10.3969/j.issn.1674-5817.2014.01.006

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (1): 29-34.DOI: 10.3969/j.issn.1674-5817.2014.01.006

三个封闭群实验兔线粒体DNA D-loop区多态性分析

申幸娇, 岳秉飞, 马丽颖

中国食品药品检定研究院, 北京 100050

收稿日期:2013-04-03 出版日期:2014-02-25 发布日期:2014-02-25
作者简介:申幸娇(1987-), 女, 硕士研究生,E-mail: xjshen816@163.com
基金资助:
“国家科技支撑计划”(2011BAI15B01;2013BAK11B01)

Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits

SHEN Xing-jiao, YUE Bing-fei, MA Li-ying

National Institutes for Food and Drug Control, Beijing 100050, China

Received:2013-04-03 Online:2014-02-25 Published:2014-02-25

摘要/Abstract

摘要： 目的通过对三个封闭群实验兔线粒体DNA控制区序列进行分析,从分子水平探讨其群体的多样性和均一性。方法利用特异引物对新西兰白兔、日本大耳白兔、青紫蓝兔三个封闭群共71份血液样品线粒体D-loop区部分序列进行扩增,纯化,测序,用Mega4.0和DNA SP软件对变异位点数、简约信息位点数、单倍型、单倍型多样度、核苷酸多样度等遗传信息进行分析。结果在71份样品中,共检测出了 6个多态性位点,多为C→G突变,定义了9种单倍型,且三个种群分别有各自单倍型,互不重叠。三个种群D-loop区多态性及种群多样性均偏低。结论本研究为兔线粒体D-Loop区序列特征和国内常用三个封闭群实验兔种群结构提供了更多数据,并为遗传育种提供指导。

关键词: 封闭群兔, 线粒体DNA, 单倍型, 多态性

Abstract: Objective To discuss the genetic diversity and uniformity in three groups of closed colony rabbits based on mitochondrial DNA control region. Methods Seventy-one blood samples from New Zealand rabbit, Japanese White rabbit and Chinchilla rabbit were collected for amplifying ,purifying and sequencing the part of sequences of mitochondrial DNA control region by PCR and Genetic Analyzer. The variation sites, Parsimony informative sites, haplotypes, haplotype diversity, nucleotide diversity were analyzed by Mega 4.0 and DNA SP. Results 6 diversity sites and 9 haplotypes were found in the seventy-one rabbits, and most mutations were C→G. Every population had its own haplotypes. The polymorphism of Mitochondrial DNA Control Region and population diversity in three group were both low. Conclusions More sequence information about mtDNA sequences and population structure of laboratory rabbits were supplied,which was helpful to genetic breeding.

Key words: Closed colony rabbits, Mitochondrial DNA, Haplotype, Genetic polymorphism

中图分类号:

Q95-33

申幸娇, 岳秉飞, 马丽颖. 三个封闭群实验兔线粒体DNA D-loop区多态性分析[J]. 实验动物与比较医学, 2014, 34(1): 29-34.

SHEN Xing-jiao, YUE Bing-fei, MA Li-ying. Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 29-34.

参考文献

[1] Larizza A, Pesole G, Reyes A, et al.Lineage specificity of the evolutionary dynamics of the mtDNA D-loop region in rodents[J]. J Mol Evol, 2002, 54(2):145-155.
[2] Casane D, Dennebouy N, de Rochambeau H, et al. Genetic analysis of systematic mitochondrial heteroplasmy in rabbit[J]. Genetics, 1994, 138:47 l-480.
[3] Hardy C, Callou C, Vigne JD, et al.Rabbit mitochondrial DNA diversity from prehistoric to modern times[J]. J Mol Evol,1995, 40:227-237.
[4] Susan J, Fuller, Peter B, et al.Limited genetic differentiation among wild Oryctolagus cuniculus L. (rabbit) populations in arid eastern Australia[J].Heredity, 1996, 77:138-145.
[5] Matthee CA, Robinson TJ.Mitochondrial DNA differentiation among geographical populations of Pronolagus rupestris, Smith's red rock rabbit (Mammalia-Lagomorpha)[J]. Heredity, 1996, 76:514-23.
[6] Casane D, Dennebouy N, de Rochambeau H, et al. Nonneutral Evolution of Tandem Repeats in the Mitochondrial DNA Control Region of Lagomorphs[J]. Mol Biol Evol, 1997, 14(8):779-789.
[7] Gissi C, Gullberg A, Arnason U.The complete mitochondrial DNA sequence of the rabbit, Oryctolagus cuniculus[J]. Genomics, 1998, 50:161-169.
[8] 王东卫, 刘伟. 实验兔线粒体DNA多态性研究[J]. 动物科学与动物医学, 1999, 16(4):3-5.
[9] Long JR, Qiu XP, Zeng FT, et al.Origin of rabbit (Oryctolagus cuniculus) in China_ evidence from mitochondrial DNA control region sequence analysis[J]. Anim Genet, 2003, 34: 82-87.
[10] Sambrook J, Russell DW.分子克隆实验指南(第三版)[M]. 北京: 科学出版社, 2002.
[11] Loparev VN, Cartas MA, Monken CE, et al.An efficient and simple method of DNA extraction from whole blood and cell lines to identify infectious agents[J]. J Virol Methods, 1991, 34(1): 105-112.
[12] 郭彦斌, 刘丑生, 王慧, 等. 利用mtDNA D-loop 区研究中国10 个绵羊品种的遗传多样性与起源[J]. 农业生物技术学报, 2012, 20(7): 799-806.
[13] 危金普, 潘学峰, 李红权, 等.单重复DNA序列在哺乳动物mtDNA D-loop区的分布及进化特征[J]. 遗传, 2011, 33(1): 67-74.
[14] 倪丽菊, 谢建云, 胡建华. 云南滇南小耳猪与广西巴马小型猪mtDNA D-Loop的初步研究[J]. 实验动物与比较医学, 2011, 31(03):207-210.
[15] 管敏强, 曹琼洁, 陈忠义, 等. 线粒体DNA序列分析封闭群小鼠遗传稳定性[J]. 实验动物与比较医学, 2009. 29(2):113-115.
[16] Mignotte F, Gueride M, Champagne AM, et al.Direct repeats in the non-coding region of rabbit mitochondrial DNA: involvement in the generation of intra- and inter-individual heterogeneity[J]. Eur J Biochem, 1990, 194:561-571.

三个封闭群实验兔线粒体DNA D-loop区多态性分析

Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵丽亚, 倪丽菊, 张彩勤, 汤建平, 姚养正, 聂艳艳, 顾晓雪, 赵莹. 基于多重PCR-LDR技术建立近交系大鼠单核苷酸多态性遗传检测方案[J]. 实验动物与比较医学, 2023, 43(5): 548-558.
[2]	魏杰, 张心妍, 王洪, 赵蓝, 刘巍, 李欢, 付瑞, 乔涵, 赵萌, 项新华, 岳秉飞. 实验小鼠核酸样品单核苷酸多态性标记检测的能力验证结果评价[J]. 实验动物与比较医学, 2022, 42(6): 505-510.
[3]	郑杰, 张国忠, 胡艳梅, 伍学一, 杨宗富, 字向东. 基于RNA-Seq技术的犏牛囊胚冷冻前后单核苷酸多态性和可变剪切分析[J]. 实验动物与比较医学, 2020, 40(4): 279-.
[4]	钱强, 徐园, 王亚恒, 周宇荀, 肖君华, 韩琳, 鲍世民, 李凯. 基于多重PCR靶向二代测序的近交系小鼠遗传质量监测方法建立[J]. 实验动物与比较医学, 2019, 39(2): 111-117.
[5]	高一龙, 贺星亮, 强京宁, 宋珍华, 温海, 陶晓冉, 孙勇, 秦海斌, 包喜军, 孟戈, 朱骞. 基于Sequenom MassARRAY SNP技术检测嗅觉受体基因与工作犬嗅探能力的关联性研究[J]. 实验动物与比较医学, 2019, 39(2): 140-145.
[6]	李银银, 陈振文, 刘丽均, 李长龙, 刘欣, 吕建祎, 郭萌, 杜小燕. 单核苷酸多态性位点法对国内近交系小鼠遗传检测[J]. 实验动物与比较医学, 2018, 38(1): 16-21.
[7]	王兴童, 陈洪岩, 韩凌霞. 抗原处理相关转运体基因多态性与疾病相关性的研究进展[J]. 实验动物与比较医学, 2017, 37(3): 252-256.
[8]	韩琳, 谢建云, 杨斐, 胡樱, 田立立, 鲍世民. 上海地区常用近交系小鼠品系的单核苷酸多态性分型研究[J]. 实验动物与比较医学, 2017, 37(1): 25-31.
[9]	赵丽亚, 张蓉, 赵莹, 邢正弘, 陈国强. 单核苷酸多态性(SNP)在近交系小鼠遗传检测中的应用[J]. 实验动物与比较医学, 2016, 36(1): 24-31.
[10]	姜宪环, 蔡慧强, 高骏, 倪丽菊, 李凯, 肖君华, 周宇荀. 用LA-PCR方法克隆东方田鼠部分Y染色体序列[J]. 实验动物与比较医学, 2012, 32(4): 324-328.
[11]	左宝芬, 杜小燕, 霍学云, 李振坤, 陈振文. 五个C57BL/6J小鼠生产群的微卫星检测及遗传学质量分析[J]. 实验动物与比较医学, 2012, 32(1): 51-55.
[12]	倪丽菊, 谢建云, 胡建华. 云南滇南小耳猪与广西巴马小型猪mtDNA D-Loop的初步研究[J]. 实验动物与比较医学, 2011, 31(3): 207-210.
[13]	赵莹, 沈志敏, 刘雄伟, 魏晓锋, 胡建华. 斑马鱼分子遗传位标及多态性研究进展[J]. 实验动物与比较医学, 2011, 31(2): 147-152.
[14]	赵玉琼1，姚春涛1，赵权2，陈华3. 三个品系小型猪PPAR-γ基因外显子1的多态性分析[J]. , 2009, 29(5): 289-292.
[15]	朱亮，应华忠，陈民利，蔡月琴，余佳，肖慧，陶涛，潘永明. 微卫星标记对封闭群和近交第五代WHBE兔的遗传分析[J]. , 2009, 29(5): 334-340.