关键词: 肝螺杆菌, 鞭毛蛋白, 克隆, 测序

Abstract: Objective The flagellin B (fla B) gene of Helicobacter hepaticus (H. hepaticus) was amplified, cloned and sequenced in order to investigate its function. Method A pair of primer was used for amplifying H. hepaticus fla B gene of BJ 0801 strain with PCR. The amplified fragment was cloned into the pGEM-T vector and transformed into competence E. coli DH5a. The recombinant plasmid was identified by PCR, endonuclease analysis and electrophoresis, and confirmed by sequencing. The nucleotide sequence was compared with the fla B gene of H. hepaticus ATCC 51449. Results The results demonstrated that nucleotide homology of H. hepaticus BJ 0801 fla B gene with H. hepaticus ATCC 51449 fla B gene was 99%. Conclusion Characterization of the H. hepaticus BJ 0801 strain at the genetic identification would be an important step toward investigating H. hepaticus fla B gene function.

Key words: Helicobacter hepaticus (H. hepaticus)、flagellin, Cloning, Sequencing