关键词: 白血病细胞, 成瘤性, 动物模型, 接种方式

Abstract: Objective To investigate biologic characters of a mouse leukemia model prepared by different ways. Methods The mouse acute lymphoblastic leukemia cell line L1210 was conventionally cultured and modulated into 1 xl07/ml. 0.1ml cell suspension was infused into every DBA/2 mouse by vena caudalis (A group), sub cutis (B group) and abdominal cavity (C group) respectively. The formation of leukemia in mouse was investigated. The amount of peripheric leucocyte in mouse blood was counted and the morphological changes were detected timely. The liver, spleen, kidney and lung of the dying mouse were kept，weighed and maken into pathological sections. Results The ratio of tumor formation was 100% in all groups, which spent 14，7 and 14 days respectively. The average survival days of the three groups were 24.4士3.05，24.8士1.79 and 18.8±1,79d days. The amount of peripheric leucocyte increased remarkably after infusion, which was (18.45±1.84)xl0⁹/L、(22.0±4.11) × 10⁹/L and (28.48±6.74)×l0⁹/Ltwo weeks later and (22.31±12.08)×l0⁹/L (A group) and (68.18±2.82)× 10⁹/L (B group) three weeks later. Leukemic cells could be found on peripheral blood smears. In pathological sections of the dying mouse’ liver and spleen, infused leukemic cell infiltration, mainly immature lymphocytes, could be found, accompanied with destroy of normal tissue and structure. A small quantity of leukemic cells were found in lung and kidney, destroy of their normal tissue and structure was not obviously and hemorrhage appeared in lung obviously. Conclusions Inoculation of LI 210 cells into DBA/2 mouse by vena caudalis，subcutis and abdominal cavity was feasible to prepare a mouse leukemia model. There were different biological characters, merits and defects in the model due to the different infusion ways. A certain infusion way should be selected according to the aims of the research.

Key words: Leukemic cell, Tumor formation, Animal model, Infusion way