小鼠胚胎移植影响因素的初步探索

doi:10.3969/j.issn.1674-5817.2020.06.004

摘要/Abstract

摘要： 目的分析小鼠品系、胚胎移植方式和移植数量对胚胎移植结果的影响,以进一步优化并提高胚胎移植效率。方法通过超数排卵及体外受精获得277个基因修饰小鼠品系的胚胎,将不同数量的胚胎移植至假孕雌鼠的单侧或双侧输卵管中,分析比较不同体外受精率品系、不同移植胚胎数及不同移植方法对窝产仔数和产仔率的影响。结果体外受精率在0%~39% 的品系组窝产仔数（4.61±1.92）和产仔率[（19.21±9.70）%]均明显低于其他各组（均P<0.01）,体外受精率60%~69%组比90%~100% 组的窝产仔率明显升高（P<0.05）。移植胚胎数较少时（5枚）,受体雌鼠能够妊娠,但窝产仔数较少,易发生食仔现象。移植胚胎数在10枚以上时,各组窝产仔数和产仔率均较高。其中,胚胎移植数量在15枚左右时产仔率最高,达到（46.67±11.56）%,明显高于其他各组（均P<0.01）,表明此时胚胎利用率最大;移植数量达到25枚时,平均窝产仔数（9.67±1.53）最多,明显高于其他各组（均P<0.01）;移植胚胎数增加到30枚后,窝产仔数（8.33±0.58）和产仔率[（27.33±2.31）%]均有所下降,表明此时已超过小鼠子宫能承受的最大负荷。单侧移植和双侧移植的窝产仔数和产仔率均未见明显差异（P>0.05）。结论体外受精率低于40%的小鼠品系的平均窝产仔数及产仔率均较低。移植胚胎数在15枚左右时,胚胎利用率最大;移植胚胎数在25枚左右时,窝产仔数最多。单双侧移植的窝产仔数和产仔率均无明显差异。

关键词: 胚胎移植, 体外受精, 影响因素, 小鼠

Abstract: Objective To analyze the influences of mouse strain, embryo transfer method and transferred embryos' quantity on the results of embryo transfer, and to further optimize and improve the efficiency of embryo transfer. Methods The embryos from 277 gene modified strains were obtained by superovulation and in vitro fertilization (IVF), then the different numbers of embryos were transferred into the oviducts of pseudo-pregnant female mice through unilateral or bilateral embryo transplantation. The effects of strains with different IVF rates, number of transplanted embryos and transfer methods on the litter size and rate were analyzed and compared. Results When the strains with IVF rate of 0%-39%, the litter size (4.61±1.92) and the litter rate [(19.21±9.70)%were significantly lower than those in other groups (both P<0.01). The litter rate was significantly higher in 60%-69% IVF rate group than that in the 90%-100% IVF rate group (P<0.05). When the number of transferred embryos was fewer than 5, the recipient female mice got pregnant, but the litter size was less, with easy occurrence of the phenomenon of cannibalism. When the number of transferred embryos was more than 10, the litter size and rate of each group were both higher. The litter rate was (46.67±11.56)% when the number of transferred embryos was about 15, which was significantly higher than those of other groups (P<0.01), indicating that the embryo utilization rate was the highest. The average litter size was significantly the largest (9.67±1.53) with P<0.01 when the number of transferred embryos was around 25. The litter size (8.33±0.58) and the litter rate [(27.33±2.31)%were both decreased when the number of embryos transferred increased to 30, indicating that the bearing capacity of uterus almost reached the maximum. There was no significant difference in litter size or rate between unilateral and bilateral transplantation (P>0.05). Conclusion The average litter size and rate are lower when the IVF rate is lower than 40%. The embryo utilization rate is the highest, when the number of transferred embryos is about 15. The litter size is the largest when the number of transferred embryos is about 25, and the bearing capacity of uterus is almost the maximum. There is no significant difference in the litter size or rate between the unilateral and bilateral transplantation.

Key words: Embryo transfer, In vitro fertilization, Influence factor, Mice

中图分类号:

Q95-33

桂飞, 杨伟伟, 孙筱品, 顾美儿. 小鼠胚胎移植影响因素的初步探索[J]. 实验动物与比较医学, 2020, 40(6): 483-488.

GUI Fei, YANG Weiwei, SUN Xiaopin, GU Meier. Preliminary Study on the Influencing Factors of Mouse Embryo Transfer[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 483-488.

图/表 2

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