实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (4): 306-309.DOI: 10.3969/j.issn.1674-5817.2013.04.012

• 论著 • 上一篇    下一篇

绿色荧光裸小鼠的选育初报

张立波, 刘彪, 田小芸, 吴正林, 周森妹, 尤金炜, 方天, 恽时锋   

  1. 南京军区南京总医院比较医学科, 南京210002
  • 收稿日期:2012-10-29 出版日期:2013-08-25 发布日期:2013-08-25
  • 作者简介:张立波(1983-),女,硕士,技师,E-mail:zlb722@163.com

A Report on Breeding of GFP Nude Mouse

ZHANG Li-bo, Liu Biao, TIAN Xiao-yun, WU Zheng-lin, ZHOU Sen-mei, YOU Jin-wei, FANG Tian, YUN Shi-feng   

  1. Nanjing General Hospital of Nanjing Military Command Comparative Medicine Department Nanjing 210002 China
  • Received:2012-10-29 Online:2013-08-25 Published:2013-08-25

摘要: 目的 选育系统表达绿色荧光蛋白的BALB/c裸小鼠,并对该小鼠各组织器官绿色荧光蛋白表达情况进行观察。方法 根据遗传学原理对C57BL/6-TgN(GFP) 小鼠与BALB/c裸小鼠进行杂交、自交和回交,建立稳定表达绿色荧光蛋白的BALB/c裸小鼠,对该小鼠遗传质量进行检测; 解剖观察胸腺生长情况以及多组织器官绿色荧光蛋白的表达情况。结果 成功选育出的BALB/c带绿色荧光蛋白的裸小鼠(简称荧光裸小鼠),外观呈黄绿色; 胸腺缺失; 在荧光显微镜下,脑、心脏、肺脏、肝脏、肾脏及胰腺等组织器官可见明显绿色荧光。结论 成功选育出BALB/c绿色荧光裸小鼠,该小鼠外观呈黄绿色,全身主要组织器官稳定表达绿色荧光蛋白。

关键词: 绿色荧光, 裸小鼠, 选育, 表达

Abstract: Objective To breed a strain of BALB/c nude mice with green fluorescence and to observe the expression of green fluorescent protein in various organs. Method The nude mice expressing stable green fluorescence were developed by hybridizing, self-breeding and backcrossing BALB/c-nu/nu male mice with C57BL/6-TgN (GFP) female mice according to the principles of genetics. The genetic quality, thymus growth and green fluorescent protein expression in multiple organs were observed. Result The GFP nude mouse in line with BALB/c mouse was successfully bred. The mouse were presented a yellow-green appearance and characterized by athymism. The green fluorescence can be seen clearly in major organs such as liver, kidneys and pancreas under a fluorescent microscope. Conclusion A BALB/c GFP nude mouse model is developed successfully, its appearance is yellow-green and its whole-body expresses the green fluorescent protein stably.

Key words: Green fluorescent, Nude mouse, Breeding, Expression

中图分类号: