鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究

doi:10.3969/j.issn.1674-5817.2018.02.002

实验动物与比较医学 ›› 2018, Vol. 38 ›› Issue (2): 86-90.DOI: 10.3969/j.issn.1674-5817.2018.02.002

鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究

李思琪^1,2, 尹海畅¹, 赵丽丽¹, 王怡平¹, 金建丽², 陈洪岩¹

1.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/黑龙江省实验动物与比较医学重点实验室,哈尔滨 150069;
2.牡丹江师范学院生命科学与技术学院,牡丹江 157011

收稿日期:2017-11-20 出版日期:2018-04-25 发布日期:2018-04-25
作者简介:李思琪(1992-),女,硕士研究生。研究方向:动物学。E-mail:swxlsqy@126.com
基金资助:
中央级公益性科研院所基本科研业务费专项(1610302016013,1610302017013);黑龙江省自然科学基金重点项目(ZD2016006);中国农业科学院基本科研业务费专项(Y2016PT41)

Up-regulated Expression and Preliminary Mechanism of Interferon γin Duck Embryonic Fibroblasts Induced by Duck Enteritis Virus

LI Si-qi^1,2, YIN Hai-chang¹, ZHAO Li-li¹, WANG Yi-ping¹, JIN Jian-li², CHEN Hong-yan¹

1. Division of Laboratory Animal and Comparative Medicine,State Key Laboratory of Veterinary Biotechnology/Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China;
2. College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China

Received:2017-11-20 Online:2018-04-25 Published:2018-04-25

摘要/Abstract

摘要： 目的探讨鸭肠炎病毒(DEV)诱导鸭胚成纤维细胞(DEF)中γ干扰素(IFNγ)的表达情况及初步机制。方法分别提取感染DEV后24 h、36 h、48 h、60 h的DEF总RNA,反转录成cDNA,使用特异性引物,运用荧光定量PCR方法,检测IFNγ及几个相关干扰素刺激基因(ISGs)mRNA水平的变化。结果感染DEV的细胞相对于未感染的细胞,IFNγ的mRNA在24 h、36 h、48 h、60 h均显著上调, 且相关ISGs 包括抗黏液病毒基因(Mx)、DEAD框蛋白50(DDX50, 是一种ATP依赖的RNA解旋酶)、2'-5'寡腺苷酸合成酶L (OASL)等基因的mRNA表达水平也均显著上调。结论 DEV感染DEF能够诱导IFNγ产生及相关ISGs表达的上调。

关键词: 鸭肠炎病毒(DEV), 鸭胚成纤维细胞(DEF), γ干扰素(IFNγ), 干扰素刺激基因 (ISGs), 上调表达

Abstract: Objective In order to investigate the mechanism of interferon γ(IFNγ) up-regulation in duck embryo fibroblasts (DEF) induced by duck enteritis virus(DEV) infection.Methods The total RNA was extracted from DEF at 24 h,36 h,48 h and 60 h after DEV infection,and reverse transcripted into cDNA.The expression of IFNγand related interferon stimulated genes (ISGs) was detected by fluorescence quantitative PCR using specific primers.Results The expression level of IFNγwas significantly up-regulated at 24 h,36 h,48 h and 60 h,and the mRNA expression levels of Myxovirus resistance (Mx)、DEAD-box protein 50(DDX50)、2’-5’oligoadenylates synthesis L (OASL) were also up-regulated in DEV-infected cells compared with those of uninfected cells.Conclusions DEV-infected DEF cells were able to induce IFNγproduction and up-regulation of related ISGs.

Key words: Duck enteritis virus(DEV), Duck embryo fibroblasts(DEF), Interferon γ(IFNγ), Interferon stimulated genes(ISGs), Up-regulated expression

中图分类号:

Q95-33

李思琪, 尹海畅, 赵丽丽, 王怡平, 金建丽, 陈洪岩. 鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究[J]. 实验动物与比较医学, 2018, 38(2): 86-90.

LI Si-qi, YIN Hai-chang, ZHAO Li-li, WANG Yi-ping, JIN Jian-li, CHEN Hong-yan. Up-regulated Expression and Preliminary Mechanism of Interferon γin Duck Embryonic Fibroblasts Induced by Duck Enteritis Virus[J]. Laboratory Animal and Comparative Medicine, 2018, 38(2): 86-90.

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鸭肠炎病毒感染诱导鸭胚成纤维细胞γ干扰素上调表达及初步机制的研究

Up-regulated Expression and Preliminary Mechanism of Interferon γin Duck Embryonic Fibroblasts Induced by Duck Enteritis Virus

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