不同浓度含铬垫料对小鼠血液学及脏器的毒性观察

doi:10.3969/j.issn.1674-5817.2019.06.006

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (6): 454-461.DOI: 10.3969/j.issn.1674-5817.2019.06.006

不同浓度含铬垫料对小鼠血液学及脏器的毒性观察

贾欢欢, 曾业文, 罗挺, 龚宝勇, 麦冬梅, 潘迎春, 赵维波

广东省实验动物监测所, 广东省实验动物重点实验室, 广州 510663

收稿日期:2019-05-27 出版日期:2019-12-25 发布日期:2021-01-05
作者简介:贾欢欢(1984-), 男, 助理研究员, 从事疾病动物模型标准化研究。E-mail: jhh@gdlami.com
共同第一作者: 曾业文(1992-), 女, 兽医师, 从事疾病动物模型标准化研究。E-mail: 1056756525@qq.com
基金资助:
广东省省级科技计划项目(2016A030303014,2017B020231001)

Effects of Different Concentrations of Chromium-containing Bedding Materials on Toxity of Blood and Organs in BALB/c Nude Mice and KM Mice

JIA Huan-huan, ZENG Ye-wen, LUO Ting, GONG Bao-yong, MAI Dong-mei, PAN Ying-chun, ZHAO Wei-bo

Guangdong Laboratory Animals Monitoring Institute, Key Laboratory of Guangdong Laboratory Animals, Guangzhou 510663, China

Received:2019-05-27 Online:2019-12-25 Published:2021-01-05

摘要/Abstract

摘要： 目的评价不同浓度含铬垫料对BALB/c裸小鼠及KM小鼠血液学及脏器的毒性影响。方法制备稳定的不同浓度含铬垫料,48只BALB/c裸小鼠及48只KM小鼠分别被随机分为4组,每组12只,雌雄各半。对照组垫料铬含量为(2.30±1.12) mg/kg、低、中和高剂量组垫料铬含量分别为(15.50±0.71) mg/kg、(71.50±3.54) mg/kg和(347.00±35.36) mg/kg。小鼠分别于不同垫料中饲养15周,每周更换1次垫料,每日对动物进行一般观察,于饲养15周采血解剖,检测血常规、血生化、脏器系数及脏器病理等。结果 BALB/c裸小鼠及KM小鼠饲养于不同铬含量垫料中15周,大体观察未见异常,动物均未见死亡,大体解剖均未见异常;与对照组比较,血常规指标及脏器系数均未见明显差异;血液生化检查发现BALB/c裸小鼠低、中、高剂量组血清中丙氨酸氨基转氨酶(ALT)及尿素氮(BUN)水平均显著升高,中、高剂量组碱性磷酸酶(ALP)、肌酐(Crea)水平显著升高,而低、高剂量组甘油三脂(TG)水平显著下降,KM小鼠血液生化指标均未见明显差异;病理检查发现,2种小鼠各剂量组均出现不同程度的肝脏局灶性炎细胞浸润、肝细胞水肿、肾脏炎性细胞浸润、睾丸曲细精管萎缩及卵巢黄体数量减少等改变。结论本实验垫料中六价铬含量达到(15.50±0.71) mg/kg及以上时可导致BALB/c裸小鼠及KM小鼠出现不同程度的肝、肾、睾丸及卵巢毒性。

关键词: 铬, 垫料, BALB/c裸小鼠, KM小鼠, 毒性

Abstract: Objective To evaluated the toxic effects of different concentrations of chromium-containing bedding materials on blood and organs of BALB/c nude mice and KM mice. Methods The stable different concentrations of chromium-containing bedding materials were prepared, 48 BALB/c nude mice and 48 KM mice were randomly divided into 4 groups respectively, 12 mice per group with half males and females, the dosage of chromium in control, low-dose, middle-does and high-does groups were (2.30±1.12) mg/kg, (15.50±0.71) mg/kg, (71.50±3.54) mg/kg and (347.00±35.36) mg/kg, respectively. BALB/c nude mice and KM mice were raised for 15 weeks with different bedding materials which were replaced once a week, general condition of mice were observed daily, the mice were dissected at the 15th week and the blood was collected. Besides, haematological and biochemical indexes of blood plasma, organ coefficient and organ pathology were detected. Results After 15 weeks with different bedding materials, no abnormalities and death were observed in BALB/c nude mice and KM mice, no significant differences of haematological index and organ coefficient were found between the four groups, and no abnormal anatomy was observed. In BALB/c nude mice, the alanine aminotransferase (ALT) and blood urea nitrogen (BUN) levels significantly increased in low, middle and high-dose groups, alkaline phosphatase(ALP) and creatinine (Crea) levels significantly increased in middle and high-dose groups, Triglyceride (TG) levels significantly decreased in low and high-dose groups. In KM mice groups, no significant differences of biochemical index between four groups were observed. The organ pathology showed varying degrees of focal inflammatory infiltrates and cellular swelling in the liver, inflammatory infiltrates in kedney, atrophic seminiferous tubular and decreasing corpus luteum in both BALB/c nude mice and KM mice. Conclusions Hexavalent chromium content greater than (15.50±0.71) mg/kg can cause varying degrees of toxicity in liver, kidney, testis and ovary of BALB/c nude mice and KM mice.

Key words: Chromium, Bedding materials, BALB/c nude mice, KM mice, Toxicity

中图分类号:

Q95-33

贾欢欢, 曾业文, 罗挺, 龚宝勇, 麦冬梅, 潘迎春, 赵维波. 不同浓度含铬垫料对小鼠血液学及脏器的毒性观察[J]. 实验动物与比较医学, 2019, 39(6): 454-461.

JIA Huan-huan, ZENG Ye-wen, LUO Ting, GONG Bao-yong, MAI Dong-mei, PAN Ying-chun, ZHAO Wei-bo. Effects of Different Concentrations of Chromium-containing Bedding Materials on Toxity of Blood and Organs in BALB/c Nude Mice and KM Mice[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 454-461.

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不同浓度含铬垫料对小鼠血液学及脏器的毒性观察

Effects of Different Concentrations of Chromium-containing Bedding Materials on Toxity of Blood and Organs in BALB/c Nude Mice and KM Mice

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