中国仓鼠口腔鳞癌miR-504的表达特征分析

doi:10.3969/j.issn.1674-5817.2019.05.002

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (5): 342-348.DOI: 10.3969/j.issn.1674-5817.2019.05.002

中国仓鼠口腔鳞癌miR-504的表达特征分析

卫佳宁, 续国强, 高继萍, 王晓堂, 肖兰飞, 宋国华

山西医科大学实验动物中心, 实验动物与人类疾病模型动物模型山西省重点实验室, 太原 030001

收稿日期:2019-03-20 出版日期:2019-10-25 发布日期:2021-01-05
作者简介:卫佳宁(1994-), 女, 硕士研究生, 研究方向: 人类疾病动物模型。E-mail: 823952957@qq.com
基金资助:
国家自然科学基金(31772551), 山西省自然科学基金(201701D121087)

Expression Characteristics of miR-504 in Oral Squamous Cell Carcinoma of Chinese Hamster

WEI Jia-ning, XU Guo-qiang, GAO Ji-ping, WANG Xiao-tang, XIAO Lan-fei, SONG Guo-hua

Shanxi Medical University Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Taiyuan 030001, China

Received:2019-03-20 Online:2019-10-25 Published:2021-01-05

摘要/Abstract

摘要： 目的构建中国仓鼠口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)动物模型,研究miR-504在中国仓鼠OSCC动物模型的癌组织和癌旁舌组织中的表达特征。方法采用二甲基苯并蒽(DMBA)涂抹法诱导中国仓鼠OSCC模型, 对OSCC中miR-504的前体序列克隆分析; 提取癌组织颊囊和癌旁组织舌的总miRNA, qRT-PCR检测不同时间点(造模0周、6周、12周和18周)中国仓鼠颊囊和舌组织中miR-504的表达水平。结果颊囊黏膜在造模6周时出现轻度的充血,12周时黏膜增厚,18周时局部有白斑样病变和糜烂。与正常对照组相比,在 6周、12周和18周时,miR-504在颊囊组织的表达水平分别增加约0.83、0.33,0.23倍(P<0.001); 而在舌组织的表达水平变化分别约为正常舌组织的0.92、0.84、0.27倍(0.001<P<0.01)。结论 DMBA涂抹仓鼠颊囊组织建立的OSCC模型,癌组织中miR-504的表达量显著增加,miR-504与OSCC的发生发展密切相关,可能调控其靶基因及其下游的信号通路。本结果有助于了解miR-504在中国仓鼠OSCC各个阶段的癌组织和癌旁组织的差异表达特征。

关键词: 中国仓鼠, 口腔鳞状细胞癌(OSCC), miR-504表达特征

Abstract: Objective An animal model was established to study the expression characteristics of miR-504 in the cancer and adjacent paracancerous tongue tissues of the Chinese hamster oral squamous cell carcinoma (OSCC). Methods The OSCC model of Chinese hamster was induced by smear of 9,10-Dimethy1-1,2-Benzanthracence (DMBA), cloning and analysis of the precursor sequence of miR-504 in OSCC. The total miRNAs in the cheek pouch and paracancerous tongue tissues were detected by qRT-PCR at different time points (0, 6, 12 and 18 weeks of modeling). Results The buccal mucosa showed mild hyperemia at 6 week, thickened at 12 week, local leukoplakia lesions and erosion at 18 week. Compared with the normal control group, the expression levels of miR-504 in the cheek pouch tissue increased by about 0.83, 0.33 and 0.23 times (P<0.001) at 6, 12, and 18 weeks respectively. The expression level in the tongue tissue was about 0.92, 0.84 and 0.27 times compared with that of normal tongue tissue respectively (0.001<P<0.01). Conclusions The expression of miR-504 was significantly increased in the OSCC model established by DMBA smear of the hamster cheek pouch tissue. miR-504 is closely related to the development of OSCC and may regulate its target gene and its downstream signaling pathway. The results of this study are helpful to understand the differential expression characteristics of miR-504 in the cancer and paracancerous groups of various stages of OSCC in Chinese hamsters.

Key words: Chinese hamster, Oral squamous cell carcinoma (OSCC), MiR-504 expression characteristics

中图分类号:

Q95-33

卫佳宁, 续国强, 高继萍, 王晓堂, 肖兰飞, 宋国华. 中国仓鼠口腔鳞癌miR-504的表达特征分析[J]. 实验动物与比较医学, 2019, 39(5): 342-348.

WEI Jia-ning, XU Guo-qiang, GAO Ji-ping, WANG Xiao-tang, XIAO Lan-fei, SONG Guo-hua. Expression Characteristics of miR-504 in Oral Squamous Cell Carcinoma of Chinese Hamster[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 342-348.

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中国仓鼠口腔鳞癌miR-504的表达特征分析

Expression Characteristics of miR-504 in Oral Squamous Cell Carcinoma of Chinese Hamster

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