食蟹猴源弗氏柠檬酸杆菌的分离鉴定及生物学特性分析

doi:10.12300/j.issn.1674-5817.2025.150

实验动物与比较医学 ›› 2026, Vol. 46 ›› Issue (3): 367-377.DOI: 10.12300/j.issn.1674-5817.2025.150

食蟹猴源弗氏柠檬酸杆菌的分离鉴定及生物学特性分析

李鹤龄¹, 钱子瑶¹, 覃刚民², 蒋德兵², 张艳琼², 金文政², 王宏¹^,²()()

^1.昆明理工大学灵长类转化医学研究院, 昆明 650500
^2.云南中科灵长类生物医学重点实验室, 昆明 650500

收稿日期:2025-09-16 修回日期:2025-12-02 出版日期:2026-06-25 发布日期:2026-06-19
通讯作者:
王宏（1975—），男，博士，研究员，研究方向：实验动物疾病的诊断与治疗。E-mail：wangh@lpbr.cn。ORCID：0009-0004-6763-3168
作者简介:李鹤龄（1990—），女，硕士，高级兽医师，研究方向：非人灵长类动物疾病的诊断与治疗。E-mail：lihl@lpbr.cn
基金资助:
云南省自然科学基金项目“灵长类生殖发育与人类重大疾病的机制及转化研究”(202001BC070001)

Isolation, Identification and Biological Characteristics Analysis of Citrobacter freundii from Cynomolgus Monkey (Macaca fascicularis)

LI Heling¹, QIAN Ziyao¹, QIN Gangmin², JIANG Debing², ZHANG Yanqiong², JIN Wenzheng², WANG Hong¹^,²()()

^1.Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China
^2.Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China

Received:2025-09-16 Revised:2025-12-02 Published:2026-06-25 Online:2026-06-19
Contact: WANG Hong (ORCID: 0009-0004-6763-3168), E-mail: wangh@lpbr.cn

摘要/Abstract

摘要：

目的对1株导致食蟹猴腹泻的致病菌进行鉴定分析、动物回归试验和药物敏感性研究，为弗氏柠檬酸杆菌的临床治疗提供实践依据。方法收集9只腹泻食蟹猴的新鲜粪便，分别划线接种于沙门-志贺氏（Salmonella-Shigella，SS）培养基、LB培养基和哥伦比亚血琼脂培养基，37 ℃恒温培养24 h。随后通过菌落形态观察、革兰氏染色、生化试验和16S rRNA基因测序对分离菌株进行鉴定，并采用PCR方法检测分离菌株的主要毒力基因；将分离菌增殖后灌胃健康食蟹猴和C57BL/6小鼠进行动物回归试验，以分析菌株的致病性；最后，采用圆片扩散法检测分离菌株的药物敏感性。结果从9只腹泻食蟹猴的粪便样本中共分离到1株细菌，命名为MF071743。该分离菌株在SS培养基上形成粉红色、光滑的圆形菌落；在LB培养基上形成光滑、湿润、表面有光泽、边缘整齐的半透明菌落；在哥伦比亚血琼脂上形成光滑湿润的灰白色菌落。革兰氏染色结果显示，该菌株为革兰氏阴性、无芽孢短杆菌。生化实验结果显示，该分离菌株动力试验为阳性，甘露醇试验、硫化氢试验、甲基红试验、枸橼酸盐利用试验、葡萄糖产气试验、棉子糖试验、山梨醇试验和D-木糖发酵试验均为阳性，但苯丙氨酸试验、葡萄糖酸盐试验、吲哚试验、伏-波（Voges Proskauer，VP）试验、脲酶试验、赖氨酸试验、鸟氨酸试验和侧金盏花醇试验均为阴性。16S rRNA基因测序结果显示，MF071743与弗氏柠檬酸杆菌基因组的相似度高达99.0%。PCR结果显示，该菌株携带编码脲酶辅助蛋白（urease accessory protein，ure）D、ureE和ureF的基因。动物灌胃实验结果显示，该菌株能导致食蟹猴粪便稀软，C57BL/6小鼠72 h内全部死亡（100%）。药敏试验结果显示，该菌株对头孢曲松、阿米卡星、庆大霉素、头孢他啶、左氧氟沙星等16种药物敏感，对氨苄西林、头孢唑林、万古霉素、红霉素和头孢氨苄耐药。结论从食蟹猴腹泻粪便中分离到1株弗氏柠檬酸杆菌，该菌株对多种抗生素具有耐药性，本研究结果为圈养实验猴消化道疾病的诊断和治疗提供了参考。

关键词: 食蟹猴, 弗氏柠檬酸杆菌, 细菌培养, 分离鉴定, 系统发育分析

Abstract:

Objective Identification and analysis, animal regression test, and drug susceptibility study were conducted on a pathogenic strain causing diarrhea in cynomolgus monkeys, aiming to provide a practical basis for the clinical treatment of Citrobacter freundii. Methods Fresh fecal samples were collected from nine diarrheal cynomolgus monkeys and streaked on Salmonella-Shigella (SS) medium, LB medium, and Columbia blood agar medium, and incubated at 37 ℃ for 24 h. Subsequently, the isolated strain was identified by colony morphology observation, Gram staining, biochemical tests and 16S rRNA gene sequencing, and PCR was used to detect its major virulence genes. After propagation, the isolate was intragastrically administered to healthy cynomolgus monkeys and C57BL/6 mice for animal regression tests to evaluate its pathogenicity. Finally, the disk-diffusion method was used to detect the antimicrobial susceptibility of the isolate. Results A single bacterial strain, designated MF071743, was isolated from 9 fecal samples collected from diarrheal cynomolgus monkeys. The isolate formed pink, smooth, round colonies on SS medium; translucent, smooth, moist, shiny colonies with neat edges on LB medium; and smooth, moist, off-white colonies on Columbia blood agar medium. Gram staining revealed that the isolate was a Gram-negative, spore-free short bacillus. Biochemical tests showed that the isolate was positive for motility test, mannitol test, hydrogen sulfide test, methyl red test, citrate utilization test, gas production from glucose test, raffinose test, sorbitol test, and D-xylose test, but negative for phenylalanine test, gluconate test, indole test, Voges Proskauer (VP) test, urease test, lysine test, ornithine test, and adonitol test. The results of 16S rRNA gene sequencing showed that the gene sequence similarity between strain MF071743 and Citrobacter freundii was 99.0%. PCR results showed that this strain carries genes encoding the urease accessory proteins UreD, UreE, and UreF. Animal intragastric administration assays demonstrated that the strain caused loose stools in cynomolgus monkeys, and all C57BL/6 mice died within 72 h. Drug susceptibility test results indicated that the isolate was sensitive to 16 antibiotics, including ceftriaxone, amikacin, gentamicin, ceftazidime, and levofloxacin, while it exhibited resistance to 5 antibiotics, including ampicillin, cefazolin, vancomycin, erythromycin, and cephalexin. Conclusion A strain of Citrobacter freundii with certain drug resistance was isolated from the feces of diarrheal cynomolgus monkeys. The results of this study provide a reference for the diagnosis and treatment of gastrointestinal diseases in captive laboratory monkeys.

Key words: Cynomolgus monkey, Citrobacter freundii, Bacterial culture, Isolation and identification, Phylogenetic analysis

中图分类号:

S852.61
S858

李鹤龄,钱子瑶,覃刚民,等. 食蟹猴源弗氏柠檬酸杆菌的分离鉴定及生物学特性分析[J]. 实验动物与比较医学, 2026, 46(3): 367-377. DOI: 10.12300/j.issn.1674-5817.2025.150.

LI Heling,QIAN Ziyao,QIN Gangmin,et al. Isolation, Identification and Biological Characteristics Analysis of Citrobacter freundii from Cynomolgus Monkey (Macaca fascicularis)[J]. Laboratory Animal and Comparative Medicine, 2026, 46(3): 367-377. DOI: 10.12300/j.issn.1674-5817.2025.150.

图/表 7

参考文献 38

[1]	陈少锋, 王河, 赵湘培. 食蟹猴在慢性病动物模型药理研究应用中的进展[J]. 药学研究, 2020, 39(9): 536-539. DOI: 10.13506/j.cnki.jpr.2020.09.009 .
	CHEN S F, WANG H, ZHAO X P. Advances in pharmacology of Cynomolgus monkeys in animal models of chronic diseases[J]. J Pharm Res, 2020, 39(9): 536-539. DOI: 10.13506/j.cnki.jpr.2020.09.009 .
[2]	王弘义, 麻秀珍, 赵国明, 等. 食蟹猴沙门氏菌和志贺氏菌带菌情况调查[J]. 中国兽医科技, 1997(8): 43-44. DOI: 10.16656/j.issn.1673-4696.1997.08.030 .
	WANG H Y, MA X Z, ZHAO G M, et al. Investigation on Salmonella and Shigella in Macaca fascicularis[J]. Chin J Vet Sci Technol, 1997(8): 43-44. DOI: 10.16656/j.issn.1673-4696.1997.08.030 .
[3]	谢永平, 贺会利, 庞彬辉. 患腹泻症状食蟹猴寄生虫和致病菌鉴定与诊断[J]. 安徽农业科学, 2023, 51(2): 104-107. DOI: 10.3969/j.issn.0517-6611.2023.02.026 .
	XIE Y P, HE H L, PANG B H. Identification and diagnosis of parasites and pathogenic bacteria in Macaca fascicularis with diarrhea symptoms[J]. J Anhui Agric Sci, 2023, 51(2): 104-107. DOI: 10.3969/j.issn.0517-6611.2023.02.026 .
[4]	邱索平, 凌华枢, 陈文. 食蟹猴肠道志贺氏菌的分离和鉴定[J]. 安徽农业科学, 2007, 35(33): 10730, 10736. DOI: 10.13989/j.cnki.0517-6611.2007.33.116 .
	QIU S P, LING H S, CHEN W. Isolation and identification of Shigella from the intestinal tract of Macaca fascicularis [J]. J Anhui Agric Sci, 2007, 35(33): 10730, 10736. DOI: 10.13989/j.cnki.0517-6611.2007.33.116 .
[5]	HE Y, YUAN X D, LI J L, et al. Citrobacter freundii caused head ulcer disease and immune response in juvenile Procambarus clarkii [J]. Fishes, 2025, 10(2): 70. DOI: 10.3390/fishes10020070 .
[6]	LESKI T A, TAITT C R, BANGURA U, et al. Finished genome sequence of the highly multidrug-resistant human urine isolate Citrobacter freundii strain SL151[J]. Genome Announc, 2016, 4(6): e01225-e01216. DOI: 10.1128/genomeA. 01225-16 .
[7]	LIU L Y, CHEN D L, LIU L Q, et al. Genetic diversity, multidrug resistance, and virulence of Citrobacter freundii from diarrheal patients and healthy individuals[J]. Front Cell Infect Microbiol, 2018, 8: 233. DOI: 10.3389/fcimb.2018.00233 .
[8]	YANAGAWA Y, HIRANO Y, MATSUMOTO N, et al. A case of emphysematous pyelonephritis induced by Citrobacter freundii infection[J]. J Emerg Trauma Shock, 2013, 6(4): 287. DOI: 10.4103/0974-2700.120380 .
[9]	DOMINGOS M O, FRANZOLIN M R, DOS ANJOS M T, et al. The influence of environmental bacteria in freshwater stingray wound-healing[J]. Toxicon, 2011, 58(2): 147-153. DOI: 10.1016/j.toxicon.2011.04.016 .
[10]	FERNÁNDEZ A, VELA A I, ANDRADA M, et al. Citrobacter freundii septicemia in a stranded newborn cuvier's beaked whale (Ziphius cavirostris)[J]. J Wildl Dis, 2011, 47(4): 1043-1046. DOI: 10.7589/0090-3558-47.4.1043 .
[11]	刘乃瑜, 王至诚, 何宏港, 等. 鳖源柠檬酸杆菌属新种的分离鉴定与多位点序列[J]. 水产学报, 2023, 47(3): 203-214. DOI: 10.11964/jfc.20210612924 .
	LIU N Y, WANG Z C, HE H G, et al. Identification and multilocus sequence analysis of a novel species of Citrobacter isolated from diseased Pelodiscus sinensis[J]. J Fish China, 2023, 47(3): 203-214. DOI: 10.11964/jfc.20210612924 .
[12]	唐海波, 陈凤莲, 饶桂波, 等. 竹鼠源弗氏柠檬酸杆菌的分离鉴定及致病性分析[J]. 畜牧兽医学报, 2017, 48(5): 922-929. DOI: 10.11843/j.issn.0366-6964.2017.05.017 .
	TANG H B, CHEN F L, RAO G B, et al. Isolation and identification of Citrobacter freundii from the diseased bamboo rats and analysis of the pathogenicity of the isolate[J]. Chin J Anim Vet Sci, 2017, 48(5): 922-929. DOI: 10.11843/j.issn.0366-6964.2017.05.017 .
[13]	徐静, 冯永其, 段俊堂, 等. 1株灰鹦鹉源弗氏柠檬酸杆菌的分离鉴定及系统发育分析[J]. 中国兽医杂志, 2020, 56(12): 60-61.
	XU J, FENG Y Q, DUAN J T, et al. Isolation, identification and phylogenetic analysis of a Citrobacter freundii strain from grey parrot[J]. Chin J Vet Med, 2020, 56(12): 60-61.
[14]	BARNES L A, CHERRY W B. A group of paracolon organisms having apparent pathogenicity[J]. Am J Public Health Nations Health, 1946, 36(5): 481-483.
[15]	许国洋, 付文贵, 付利芝, 等. 弗氏柠檬酸杆菌的鉴定及其药敏试验[J]. 动物医学进展, 2024, 45(4): 92-96. DOI: 10.16437/j.cnki.1007-5038.2024.04.010 .
	XU G Y, FU W G, FU L Z, et al. Identification and drug sensitivity test of Citrobacter freundii [J]. Prog Vet Med, 2024, 45(4): 92-96. DOI: 10.16437/j.cnki.1007-5038.2024.04.010 .
[16]	孙洋, 郭学军, 周博, 等. 东北虎出血性腹泻病原分离与鉴定[J]. 中国兽医杂志, 2008, 44(9): 89-90. DOI: 10.3969/j.issn.0529-6005.2008.09.024 .
	SUN Y, GUO X J, ZHOU B, et al. Isolation and identification of pathogens causing hemorrhagic diarrhea in Siberian tiger[J]. Chin J Vet Med, 2008, 44(9): 89-90. DOI: 10.3969/j.issn.0529-6005.2008.09.024 .
[17]	雷静, 练耕宁, 牟平, 等. 从犊牛腹泻病料中分离到两株弗劳地枸橼酸盐杆菌的报告[J]. 中国奶牛, 2007(7): 33-34. DOI: 10.3969/j.issn.1004-4264.2007.07.014 .
	LEI J, LIAN G N, MOU P, et al. Report on Two strains of Citrobacter freundii isolated from calves with diarrhea[J]. China Dairy Cattle, 2007(7): 33-34. DOI: 10.3969/j.issn.1004-4264.2007.07.014 .
[18]	周勤, 郭光远. 弗劳地枸橼酸杆菌引起的猕猴腹泻[J]. 上海实验动物科学, 2004, 24(1): 39-40. DOI: 10.3969/j.issn.1674-5817.2004.01.011 .
	ZHOU Q, GUO G Y. Identification of Citrobacter freundii caused diarrhea in Rhesus monkey[J]. Shanghai Lab Anim Sci, 2004, 24(1): 39-40. DOI: 10.3969/j.issn.1674-5817.2004.01.011 .
[19]	LOGANATHAN M, THANGAVELU R, PUSHPAKANTH P, et al. First report of rhizome rot of banana caused by Klebsiella variicola in India[J]. Plant Dis, 2021, 105(7): 2011. DOI: 10.1094/pdis-10-20-2316-pdn .
[20]	肖宁, 孔令严, 周昊, 等. 克氏原螯虾病原弗氏柠檬酸杆菌的分离鉴定及其药敏与黏附特性[J]. 水产学报, 2016, 40(6): 946-955. DOI: 10.11964/jfc.20150910088 .
	XIAO N, KONG L Y, ZHOU H, et al. Isolation and identification of Citrobacter freundii from diseased Procambarus clarkii and its antibiotics susceptibility and adhesion characteristics[J]. J Fish China, 2016, 40(6): 946-955. DOI: 10.11964/jfc.20150910088 .
[21]	东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 156-158.
	DONG X Z, CAI M Y. Manual for Systematic Identification of Common Bacteria[M]. Beijing: Science Press, 2001: 156-158.
[22]	李雨琪, 许静茹, 朱智豪, 等. 五株华南虎源弗氏柠檬酸杆菌的分离鉴定及其生物学特性研究[J]. 中国兽医科学, 2024, 54(1): 70-78. DOI: 10.16656/j.issn.1673-4696.2024.0018 .
	LI Y Q, XU J R, ZHU Z H, et al. Isolation, identification and biological characteristics of five strains of Citrobacter fredii from South China tiger[J]. Chin Vet Sci, 2024, 54(1): 70-78. DOI: 10.16656/j.issn.1673-4696.2024.0018 .
[23]	黄莉萍, 安莉丽, 李芳, 等. 中华鳖弗氏柠檬酸杆菌的鉴定及病理组织观察[J]. 浙江农业学报, 2020, 32(7): 1176-1186. DOI: 10.3969/j.issn.1004-1524.2020.07.06 .
	HUANG L P, AN L L, LI F, et al. Identification and pathological observation of Citrobacter freundii from Pelodiscus sinensis [J]. Acta Agric Zhejiangensis, 2020, 32(7): 1176-1186. DOI: 10.3969/j.issn.1004-1524.2020.07.06 .
[24]	THANIGAIVEL S, VIJAYAKUMAR S, GOPINATH S, et al. In vivo and in vitro antimicrobial activity of Azadirachta indica (Lin) against Citrobacter freundii isolated from naturally infected Tilapia (Oreochromis mossambicus)[J]. Aquaculture, 2015, 437: 252-255. DOI: 10.1016/j.aquaculture.2014.12.008 .
[25]	ANDERSON M T, MITCHELL L A, ZHAO L L, et al. Citrobacter freundii fitness during bloodstream infection[J]. Sci Rep, 2018, 8(1): 1-14. DOI: 10.1038/s41598-018-30196-0 .
[26]	刘会胜, 赵战勤, 薛云, 等. 羊源致病性弗氏柠檬酸杆菌的分离与鉴定[J]. 畜牧兽医学报, 2015, 46(9): 1593-1599. DOI: 10.11843/j.issn.0366-6964.2015.09.014 .
	LIU H S, ZHAO Z Q, XUE Y, et al. Isolation and identification of Citrobacter freundii from the diseased small tail Han sheep[J]. Chin J Anim Vet Sci, 2015, 46(9): 1593-1599. DOI: 10.11843/j.issn.0366-6964.2015.09.014 .
[27]	SCHMIDT H, MONTAG M, BOCKEMÜHL J, et al. Shiga-like toxin Ⅱ-related cytotoxins in Citrobacter freundii strains from humans and beef samples[J]. Infect Immun, 1993, 61(2): 534-543. DOI: 10.1128/iai.61.2.534-543.1993 .
[28]	GUARINO A, GIANNELLA R, THOMPSON M R. Citrobacter freundii produces an 18-amino-acid heat-stable enterotoxin identical to the 18-amino-acid Escherichia coli heat-stable enterotoxin (ST Ia)[J]. Infect Immun, 1989, 57(2): 649-652. DOI: 10.1128/iai.57.2.649-652.1989 .
[29]	李华, 邢殿楼, 白国福, 等. 弗氏柠檬酸杆菌对河蟹致病性的研究[J]. 水生生物学报, 2001, 25(3): 217-223. DOI: 10.3321/j.issn: 1000-3207.2001.03.003 .
	LI H, XING D L, BAI G F, et al. Studies on disease of mitten crab caused by Citrobacter freudii[J]. Acta Hydrobiol Sin, 2001, 25(3): 217-223. DOI: 10.3321/j.issn: 1000-3207.2001.03.003 .
[30]	阎斌伦, 张晓君, 梁利国, 等. 三疣梭子蟹病原弗氏柠檬酸杆菌的分离鉴定及定居因子抗原基因检测[J]. 水产学报, 2012, 36(3): 391-398. DOI: 10.3724/SP.J.1231.2012.27122 .
	YAN B L, ZHANG X J, LIANG L G, et al. Detection of cfa gene and isolation and identification of the pathogen Citrobacter freundii isolated from Portunus trituberculatus [J]. J Fish China, 2012, 36(3): 391-398. DOI: 10.3724/SP.J.1231.2012.27122 .
[31]	李常挺, 王乐平, 陶立, 等. 1株鹅源柠檬酸杆菌的分离鉴定及多位点序列分型研究[J]. 黑龙江畜牧兽医, 2023(22): 69-75, 141-142. DOI: 10.13881/j.cnki.hljxmsy.2022.11.0176 .
	LI C T, WANG L P, TAO L, et al. Isolation and identification of one strain of goose-derived Citrobacter and study on its multiple-site sequence typing[J]. Heilongjiang Anim Sci Vet Med, 2023(22): 69-75, 141-142. DOI: 10.13881/j.cnki.hljxmsy.2022.11.0176 .
[32]	GRAHL M V C, UBERTI A F, BROLL V, et al. Proteus mirabilis urease: unsuspected non-enzymatic properties relevant to pathogenicity[J]. Int J Mol Sci, 2021, 22(13): 1-17. DOI: 10.3390/ijms22137205 .
[33]	肖双燕, 韦冬冬, 刘明珠, 等. 广西地区美国牛蛙源弗氏柠檬酸杆菌的分离鉴定、耐药性及组织病理[J]. 水产学报, 2024, 48(5): 118-130. DOI: 10.11964/jfc.20230413973 .
	XIAO S Y, WEI D D, LIU M Z, et al. Isolation, identification, drug resistance and histopathology of Citrobacter freundii from bullfrog(Rana catesbeiana) in Guangxi[J]. J Fish China, 2024, 48(5): 118-130. DOI: 10.11964/jfc.20230413973 .
[34]	DEAL E N, MICEK S T, RITCHIE D J, et al. Predictors of in-hospital mortality for bloodstream infections caused by Enterobacter species or Citrobacter freundii [J]. Pharmaco-therapy, 2007, 27(2): 191-199. DOI: 10.1592/phco. 27.2.191 .
[35]	LIU L H, WANG N Y, WU A Y, et al. Citrobacter freundii bacteremia: Risk factors of mortality and prevalence of resistance genes[J]. J Microbiol Immunol Infect, 2018, 51(4): 565-572. DOI: 10.1016/j.jmii.2016.08.016 .
[36]	中华人民共和国国家卫生健康委员会. 抗菌药物临床应用指导原则(2015年版):国卫办医发〔2015〕43号[A/OL]. 2015-08-27. .
	National Health Commission of the People's Republic of China. Guidelines for clinical application of antibacterial agents (2015 edition): Document No. 43 (2015) of the Medical Administration of the General Office of the National Health and Family Planning Commission[A/OL]. 2015-08-27. .
[37]	张冬星, 康元环, 田佳鑫, 等. 团头鲂致病性弗氏柠檬酸杆菌的分离鉴定及药敏试验[J]. 中国兽医科学, 2016, 46(12): 1589-1595. DOI: 10.16656/j.issn.1673-4696.2016.12.018 .
	ZHANG D X, KANG Y H, TIAN J X, et al. Isolation, identification and drug susceptibility of the pathogenic Citrobacter freundii from Megalobrama amblycephala [J]. Chin Vet Sci, 2016, 46(12): 1589-1595. DOI: 10.16656/j.issn.1673-4696.2016.12.018 .
[38]	陈绮梨, 常藕琴, 张德峰, 等. 加州鲈源弗氏柠檬酸杆菌的分离鉴定及耐药性分析[J]. 南方农业学报, 2021, 52(2): 465-474. DOI: 10.3969/j.issn.2095-1191.2021.02.023 .
	CHEN Q L, CHANG O Q, ZHANG D F, et al. Isolation, identification and drug resistance analysis of Citrobacter freundii from Micropterus salmoides [J]. J South Agric, 2021, 52(2): 465-474. DOI: 10.3969/j.issn.2095-1191.2021.02.023 .

引物名称 Primer name	引物序列(5'→3') Primer sequence (5'→3')		退火温度/℃ Annealing temperature/℃	预期长度/bp Expected length/bp
16S rRNA	F: AGAGTTTGATCCTGGCTCAG	R: CGGTTACCTTGTTACGACTT	55.0	1 500
cfa	F: TCCAGCGCATTCA	R: TCCAGCCTTCGGCAAACG	60.5	100
ureABC	F: ATGGAACTGACTCCCAGAG	R: TCAAAACAGAAAATAGCGTTGCGCC	56.5	2 328
ureD	F: CCGCAAGAGGACGTTTGC	R: CCGACCCAGGGATGATGT	60.0	459
ureE	F: ACCCAACGCCTTGACCAC	R: AGGATTTGCAGCGGAACG	63.7	293
ureF	F: TGGAAATCGGCTGGGTGA	R: CCCGACTGCGTTCTTCATC	65.0	210
ureG	F: ATCAGCGCATTCTCACCG	R: CAATAATCGTCGCCAGACC	53.2	438

引物名称 Primer name	引物序列(5'→3') Primer sequence (5'→3')		退火温度/℃ Annealing temperature/℃	预期长度/bp Expected length/bp
16S rRNA	F: AGAGTTTGATCCTGGCTCAG	R: CGGTTACCTTGTTACGACTT	55.0	1 500
cfa	F: TCCAGCGCATTCA	R: TCCAGCCTTCGGCAAACG	60.5	100
ureABC	F: ATGGAACTGACTCCCAGAG	R: TCAAAACAGAAAATAGCGTTGCGCC	56.5	2 328
ureD	F: CCGCAAGAGGACGTTTGC	R: CCGACCCAGGGATGATGT	60.0	459
ureE	F: ACCCAACGCCTTGACCAC	R: AGGATTTGCAGCGGAACG	63.7	293
ureF	F: TGGAAATCGGCTGGGTGA	R: CCCGACTGCGTTCTTCATC	65.0	210
ureG	F: ATCAGCGCATTCTCACCG	R: CAATAATCGTCGCCAGACC	53.2	438

检测项目 Test items	结果 Results	标准菌株 Reference strains
甘露醇试验 Mannitol test	+	+
硫化氢试验 H₂S test	+	±
苯丙氨酸试验 Phenylalanine test	-	-
葡萄糖酸盐试验 Gluconate test	-	-
吲哚试验 Indigo test	-	±
VP试验 Voges Proskauer test	-	-
甲基红试验 Methyl red test	+	+
枸橼酸盐试验 Citrate test	+	±
脲酶试验 Urease test	-	±
葡萄糖产气试验 Glucose gas production test	+	±
赖氨酸试验 Lysine test	-	-
鸟氨酸试验 Ornithine test	-	±
棉子糖试验 Raffinose test	+	±
山梨醇试验 Sorbitol test	+	±
侧金盏花醇试验 Adonitol test	-	-
木糖试验 Xylose test	+	±

检测项目 Test items	结果 Results	标准菌株 Reference strains
甘露醇试验 Mannitol test	+	+
硫化氢试验 H₂S test	+	±
苯丙氨酸试验 Phenylalanine test	-	-
葡萄糖酸盐试验 Gluconate test	-	-
吲哚试验 Indigo test	-	±
VP试验 Voges Proskauer test	-	-
甲基红试验 Methyl red test	+	+
枸橼酸盐试验 Citrate test	+	±
脲酶试验 Urease test	-	±
葡萄糖产气试验 Glucose gas production test	+	±
赖氨酸试验 Lysine test	-	-
鸟氨酸试验 Ornithine test	-	±
棉子糖试验 Raffinose test	+	±
山梨醇试验 Sorbitol test	+	±
侧金盏花醇试验 Adonitol test	-	-
木糖试验 Xylose test	+	±

药物 Drug	药物含量/（µg·片^-1) Drug dose /(µg·disc^-1)	抑菌圈直径/mm Inhibition zone diameter/mm	判定结果 Assessment result	判定标准/mm Judgement standard/mm
药物 Drug	药物含量/（µg·片^-1) Drug dose /(µg·disc^-1)	抑菌圈直径/mm Inhibition zone diameter/mm	判定结果 Assessment result	R	I	S
头孢曲松 Ceftriaxone	30	30	S	≤13	14～22	≥23
阿米卡星 Amikacin	30	24	S	≤14	15～16	≥17
庆大霉素 Gentamicin	10±2.5	19	S	≤12	13～14	≥15
氨苄西林 Ampicillin	10	10	R	≤13	14～16	≥17
头孢唑啉 Cefazolin	30	7	R	≤14	15～17	≥18
头孢他啶 Ceftazidime	30	26	S	≤17	18～20	≥21
左氧氟沙星 Levofloxacin	5	28	S	≤13	14～16	≥17
万古霉素 Vancomycin	30	7	R	≤9	10～11	≥12
复方磺胺甲𫫇唑 Co-trimoxazole	23.75	24	S	≤10	11～15	≥16
环丙沙星 Ciprofloxacin	5	29	S	≤15	16～20	≥21
诺氟沙星 Norfloxacin	10	27	S	≤12	13～16	≥17
红霉素 Erythromycin	15	7	R	≤13	14～22	≥23
利福平 Rifampicin	5	7	I	≤6	7～9	≥10
阿莫西林 Amoxicillin	10	10	S	≤5	6～9	≥10
呋喃唑酮 Furazolidone	300	18	S	≤5	6～9	≥10
林可霉素 Lincomycin	2	7	I	≤5	6～11	≥12
多黏菌素B Polymyxin B	300	13	S	≤8	9～11	≥12
头孢哌酮 Cefoperazone	75	31	S	≤15	16～20	≥21
头孢氨苄 Cephalexin	30	7	R	≤13	14～16	≥17
氧氟沙星 Ofloxacin	5	28	S	≤12	13～15	≥16
杆菌肽 Bacitracin	0.04	7	I	≤6	7～9	≥10
亚胺培南 Imipenem	10	23	S	≤19	20～22	≥23
美罗培南 Meropenem	10	28	S	≤19	20～22	≥23
阿奇霉素 Azithromycin	15	18	S	≤12	–	≥13

食蟹猴源弗氏柠檬酸杆菌的分离鉴定及生物学特性分析

Isolation, Identification and Biological Characteristics Analysis of Citrobacter freundii from Cynomolgus Monkey (Macaca fascicularis)

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