一套无抓捕应激的实验狨猴椅设计及其初步应用

doi:10.12300/j.issn.1674-5817.2024.097

实验动物与比较医学 ›› 2025, Vol. 45 ›› Issue (1): 67-72.DOI: 10.12300/j.issn.1674-5817.2024.097

一套无抓捕应激的实验狨猴椅设计及其初步应用

徐聖业¹^,²(), 黄俊锋¹^,³, 陈一航¹^,², 常亮堂¹()()

^1.中国科学院脑科学与智能技术卓越创新中心实验动物中心, 上海 200031
^2.南京医科大学基础医学院, 南京 210029
^3.临港实验室, 上海 201602

收稿日期:2024-07-13 修回日期:2024-10-16 出版日期:2025-03-12 发布日期:2025-02-25
通讯作者: 常亮堂（1982—），男，硕士，高级实验师，研究方向：灵长类行为实验研究。E-mail：changliangtang@ion.ac.cn。ORCID: 0009-0000-5308-3792
作者简介:徐聖业（1996—），男，博士研究生，研究方向：灵长类语音交流神经机制研究。E-mail：907092079@qq.com

Design of a Capture Stress-Free Marmoset Monkey Chair Device for Experiments and Its Preliminary Application

XU Shengye¹^,²(), HUANG Junfeng¹^,³, CHEN Yihang¹^,², CHANG Liangtang¹()()

^1.Laboratory Animal Center, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
^2.School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China
^3.Lingang Laboratory, Shanghai 201602, China

Received:2024-07-13 Revised:2024-10-16 Published:2025-02-25 Online:2025-03-12
Contact: CHANG Liangtang (ORCID: 0009-0000-5308-3792), E-mail: changliangtang@ion.ac.cn

摘要/Abstract

摘要：

目的为避免实验猴因直接抓捕引发应激反应的问题，提高狨猴在行为学、双光子成像和电生理等实验中的适应性和实验效率，研制一种无须抓捕即可将狨猴保定的实验猴椅装置。方法通过3D图形设计和打印，制作一套可在狨猴实验时配合使用的转运笼和猴椅。首先将转运笼与实验饲养笼的食盆出口对接，轻微驱赶狨猴进入转运笼，之后将转运笼与猴椅连接，再次轻微驱赶狨猴进入猴椅，保定后进行后续实验。通过观察转运和保定的时间效率、狨猴的配合度以及应激反应等进行有效性测试和改进。结果经过测试与改进，该装置可以在无须抓捕的情况下，顺利完成狨猴的保定，显著提高了实验的流畅度和效率。随着操作次数的增加，狨猴会更加配合，操作速度加快，实验效率得到了显著提升。使用该装置后，实验狨猴的应激反应明显减少。特别是与传统的抓捕方法相比，使用该装置能显著减少狨猴的焦虑和不适，提高了其在实验中的配合度。结论本团队设计的猴椅装置能够在无须抓捕的情况下轻松实现狨猴的保定，在确保后续实验顺利进行的同时，还能保障动物福利。该装置具有操作简便、实用性强、成本低等优点，易于推广使用。

关键词: 普通狨猴, 猴椅, 保定, 应激

Abstract:

Objective To avoid stress responses in experimental monkeys caused by direct capture, and to improve the adaptability and experimental efficiency of marmosets in behavioral, two-photon imaging, and electrophysiological experiments, a device for immobilizing marmosets without the need for capture is developed. Methods A set of compatible transport cage and monkey chair was produced through 3D graphic design and printing. First, the transport cage was aligned with the feeding outlet of the experimental housing cage, and the marmoset was gently guided into the transport cage. Then, the transport cage was connected to the monkey chair, and the marmoset was gently guided into the chair for immobilization. Subsequent experiments were carried out afterward. The effectiveness was evaluated by observing the efficiency of transport and immobilization, the marmoset cooperation level, and stress responses. Results After testing and improvements, the device successfully completed immobilization of marmosets without the need for capture, significantly improving the fluency and efficiency of the experiment. As the number of operations increased, the marmosets became more cooperative, and the operation speed was significantly enhanced. After using the device, the stress responses were noticeably reduced, with marmosets showing lower stress levels. In particular, compared to traditional capture methods, the use of this device significantly reduced marmoset anxiety and discomfort, increasing their cooperation levels during the experiment. Conclusion The monkey chair device designed allows for restraint of marmosets without the need for capture, ensuring smooth progress of subsequent experiments while also safeguarding animal welfare. This device is easy to operate, highly practical, cost-effective, and has great potential for widespread application.

Key words: Common marmoset, Monkey chair, Restraint device, Stress

中图分类号:

Q95-33

徐聖业,黄俊锋,陈一航,等. 一套无抓捕应激的实验狨猴椅设计及其初步应用[J]. 实验动物与比较医学, 2025, 45(1): 67-72. DOI: 10.12300/j.issn.1674-5817.2024.097.

XU Shengye,HUANG Junfeng,CHEN Yihang,et al. Design of a Capture Stress-Free Marmoset Monkey Chair Device for Experiments and Its Preliminary Application[J]. Laboratory Animal and Comparative Medicine, 2025, 45(1): 67-72. DOI: 10.12300/j.issn.1674-5817.2024.097.

图/表 2

图1 转运笼和猴椅的结构示意图注：A，转运笼前视图；B，转运笼左视图；C，转运笼俯视图；D，转运笼侧视图；E，推板；F，猴椅前视图；G，猴椅左视图；H，猴椅俯视图；I，猴椅侧视图；J，防冲罩。1，挂钩；2，转运笼后挡板；3，可移动挂钩配套螺母固定块；4，挡板锁紧螺母孔；5，提手；6，转运笼主体；7，圆形观察孔；8，转运笼前挡板；9，猴椅前挡板；10，猴椅后挡板；11，猴椅主体；12，猴椅卡板；13，可升降底座；14，猴椅固定脚。

Figure 1 Schematic diagram of the transport cage and monkey chairNote： A， Front view of the transport cage； B， Left view of the transport cage； C， Top view of the transport cage； D， Side view of the transport cage； E， Push plate； F， Front view of the monkey chair； G， Left view of the monkey chair； H， Top view of the monkey chair； I， Side view of the monkey chair； J， Anti-collision cover. 1， Hook； 2， Rear panel of the transport cage； 3， Movable hook nut fixture block； 4， Locking nut hole for the panel； 5， Handle； 6， Main body of the transport cage； 7， Circular observation hole； 8， Front panel of the transport cage； 9， Front panel of the monkey chair； 10， Rear panel of the monkey chair； 11， Main body of the monkey chair； 12， Monkey chair clamping plate； 13， Adjustable base； 14， Fixed legs of the monkey chair.

图2 转运笼和猴椅的实体照片注：A，转运笼；B，推板；C，猴椅；D，防冲罩。

Figure 2 Photos of the transport cage and monkey chairNote： A， Transport cage； B， Push plate； C， Monkey chair； D， Anti-collision cover.

参考文献 6

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一套无抓捕应激的实验狨猴椅设计及其初步应用

Design of a Capture Stress-Free Marmoset Monkey Chair Device for Experiments and Its Preliminary Application

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