慢性束缚应激对小鼠认知功能及海马不同亚区星形胶质细胞的影响

doi:10.3969/j.issn.1674-5817.2016.03.002

实验动物与比较医学 ›› 2016, Vol. 36 ›› Issue (3): 168-173.DOI: 10.3969/j.issn.1674-5817.2016.03.002

慢性束缚应激对小鼠认知功能及海马不同亚区星形胶质细胞的影响

王彦永^1,2, 张忠霞^1,2, 孙美玉¹, 王铭维^1,2

1.河北医科大学第一医院神经内科, 石家庄 050031;
2.河北省脑老化与认知神经科学实验室, 石家庄050031

收稿日期:2016-01-19 出版日期:2016-06-25 发布日期:2016-06-25
作者简介:王彦永(1976-), 男, 硕士, 副主任医师, 副教授。E-mail: wyong7673@sohu.com
基金资助:
河北省应用基础研究计划重点基础研究项目 (14967725D)

Impacts of Chronic Restraint Stress on Cognitive Function and Astrocytes in Different Subregions of Hippocampus in Mice

WANG Yan-yong, ZHANG Zhong-xia, SUN Mei-yu, WANG Ming-wei

1. Department of Neurology, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China;
2. Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, China

Received:2016-01-19 Online:2016-06-25 Published:2016-06-25

摘要/Abstract

摘要： 目的探讨慢性束缚应激(CRS)对小鼠认知功能的影响,观察海马不同亚区(CA1、CA3和DG区)星形胶质细胞(AS)的激活情况,明确慢性应激对海马的损伤是否存在特异性靶点。方法雄性KM小鼠24只按体质量随机分为对照组和应激组,每组各12只。采用慢性束缚方法建立小鼠CRS模型,利用新异物体识别实验(NORT)和Morris水迷宫(MWM)评测其认知功能,HE染色观察细胞的形态结构变化,免疫组织化学染色法对海马CA1、CA3和DG区AS标志物胶质纤维酸性蛋白(GFAP)进行染色,采用显微镜和图像分析软件对海马上述亚区AS进行计数并分析GFAP的表达。结果与对照组相比,应激组小鼠NORT及MWM实验的成绩均显著下降(P<0.05),海马神经元形态结构损伤,CA1和CA3区AS细胞数及GFAP表达均显著增加(P<0.05),DG区两者均无显著变化(P>0.05)。结论 CRS能够导致小鼠认知功能障碍,海马CA1、CA3区AS的活化可能是其机制之一。

关键词: 慢性束缚应激(CRS), 海马, 星形胶质细胞(AS), 胶质纤维酸性蛋白(GFAP)

Abstract: Objective To explore the impact of chronic restraint stress (CRS) on the cognitive function of mice, and observe the activation of astrocyte (AS) in different subregions of hippocampus (CA1, CA3 and DG), thus to make clear whether the damage of chronic stress in hippocampus has specific targets. Methods According to the body mass, 24 male Kunming mice were randomly divided into control group and stress group, with 12 mice in each group. A chronic stress model of mouse was established by using chronic restraint method. The cognitive function was evaluated by novel object recognition test (NORT) and Morris water maze (MWM), cell morphology changes were observed by HE staining, while the glial fibrillary acidic protein (GFAP) as marker of AS in hippocampal CA1, CA3 and DG areas were observed by immunohistochemistry staining. The AS number and GFAP expression in subregions of hippocampus were counted and analyzed by using the microscope and image analysis software respectively. Results Compared with the control group, the NORT and MWM scores of stress group were both decreased significantly (P<0.05), AS numbers and GFAP expression in CA1, CA3 areas were both significantly increased (P<0.05), and in DG area there was no significant changes (P>0.05). Conclusion CRS could lead to cognitive dysfunction in mice, and the activation of AS in hippocampus CA1, CA3 regions may be one of the mechanisms.

Key words: Chronic restraint stress (CRS), Hippocampus, Astrocyte (AS), Glial fiber acidic protein (GFAP)

中图分类号:

R743Q95-33

王彦永, 张忠霞, 孙美玉, 王铭维. 慢性束缚应激对小鼠认知功能及海马不同亚区星形胶质细胞的影响[J]. 实验动物与比较医学, 2016, 36(3): 168-173.

WANG Yan-yong, ZHANG Zhong-xia, SUN Mei-yu, WANG Ming-wei. Impacts of Chronic Restraint Stress on Cognitive Function and Astrocytes in Different Subregions of Hippocampus in Mice[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 168-173.

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慢性束缚应激对小鼠认知功能及海马不同亚区星形胶质细胞的影响

Impacts of Chronic Restraint Stress on Cognitive Function and Astrocytes in Different Subregions of Hippocampus in Mice

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