Progress and Evaluation of Animal Model of Heart Qi-Yin Deficiency Syndrome

doi:10.12300/j.issn.1674-5817.2024.176

Abstract

Abstract:

Animal models combining disease and syndrome are important research tools to explore the nature of traditional Chinese medicine (TCM) syndromes. At present, the construction and evaluation methods of animal models have preliminarily established the foundation for standardized development. Qi-yin deficiency syndrome is a common type of TCM syndrome in cardiovascular diseases. It is an important pathogenic factor causing the onset, pathological damage, and chronic nature of cardiovascular diseases, as well as triggering other illnesses. The establishment of an animal model of cardiovascular disease with the characteristics of Qi-yin deficiency, along with an objective and standardized evaluation system, has become an important part of modern cardiovascular disease research. In recent years, research on the construction and evaluation of animal models of heart Qi-yin deficiency syndrome has increased, but the construction methods and evaluation criteria vary. Compared with other animal models, the literature is limited, lacking statistics and overall analysis. Therefore, based on the scientific connotation of heart Qi-yin deficiency syndrome, this article systematically reviews the evaluation system of its animal model, covering multidimensional methods such as macroscopic characterization assessment, physicochemical indicators and objective evaluation, and syndrome differentiation based on prescriptions. The specific model construction strategies are described, including single-factor induction methods (sleep deprivation, chronic intermittent hypoxia, arterial occlusion, high-salt feeding) and the compound-factor induction methods (sleep deprivation combined with drug method, chronic intermittent hypoxia combined with drug method, exhaustive swimming combined with drug method). Meanwhile, application examples of each model in the research are listed, the existing problems in the current model construction and evaluation are analyzed, and optimization directions are proposed, such as promoting the compound factor induction strategy and improving the objectivity of the evaluation criteria. This article aims to provide theoretical references for constructing an animal model of heart Qi-yin deficiency syndrome that conforms to TCM characteristics, and thereby laying a scientific foundation for the prevention and treatment of cardiovascular diseases with TCM.

Key words: Cardiovascular disease, Qi-yin deficiency syndrome, Animal models, Combination of disease and syndrome

CLC Number:

R285.5

LIU Yayi,JIA Yunfeng,ZUO Yiming,et al. Progress and Evaluation of Animal Model of Heart Qi-Yin Deficiency Syndrome[J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 411-421. DOI: 10.12300/j.issn.1674-5817.2024.176.

Figures/Tables 2

References 49

[1]	刘明波, 何新叶, 杨晓红, 等. «中国心血管健康与疾病报告2023»概要(心血管疾病流行及介入诊疗状况)[J]. 中国介入心脏病学杂志, 2024, 32(10):541-550. DOI: 10.3969/j.issn.1004-8812.2024.10.001 .
	LIU M B, HE X Y, YANG X H, et al. Interpretation of report on cardiovascular health and diseases in China 2023[J]. Chin J Interv Cardiol, 2024, 32(10):541-550. DOI: 10.3969/j.issn.1004-8812.2024.10.001 .
[2]	李缘缘, 高碧珍. 中医证动物模型的发展现状、问题与对策思考[J].中华中医药杂志, 2024, 39(1):49-53.
	LI Y Y, GAO B Z. Development status, problems and countermeasures of animal models of traditional Chinese medicine[J]. China J Tradit Chin Med Pharm, 2024, 39(1):49-53.
[3]	董雨蓉, 解存, 刘畅, 等. 基于病证结合的冠心病动物模型制备研究进展与思考[J]. 天津中医药, 2024, 41(2):264-272. DOI: 10.11656/j.issn.1672-1519.2024.02.20 .
	DONG Y R, XIE C, LIU C, et al. Research progress and thinking of coronary heart disease animal model based on the combination of traditional Chinese medicine disease and syndrome[J]. Tianjin J Tradit Chin Med, 2024, 41(2):264-272. DOI: 10.11656/j.issn.1672-1519.2024.02.20 .
[4]	LEUNG A Y L, ZHANG J L, CHAN C Y, et al. Validation of evidence-based questionnaire for TCM syndrome differentiation of heart failure and evaluation of expert consensus[J]. Chin Med, 2023, 18(1):70. DOI:10.1186/s13020-023-00757-1 .
[5]	赵琼, 严石林, 陈为, 等. 论气阴两虚证发展源流[J]. 中国中医基础医学杂志, 2010, 16(10):853-854, 860. DOI: 10.19945/j.cnki.issn.1006-3250.2010.10.001 .
	ZHAO Q, YAN S L, CHEN W, et al. The origin and development on syndrome of Qi-yin deficiency[J]. Chin J Basic Med Tradit Chin Med, 2010, 16(10):853-854, 860. DOI: 10.19945/j.cnki.issn.1006-3250.2010.10.001 .
[6]	张伯礼, 吴勉华. 中医内科学[M]. 北京: 中国中医药出版社, 2017:87-113.
	ZHANG B L, WU M H. Internal medicine of traditional Chinese medicine[M]. Beijing: China Press of Chinese Medicine, 2017:87-113.
[7]	吴昆. 医方考[M]. 北京: 人民卫生出版社, 2023: 325.
	WU K. Medical formula examination[M]. Beijing: People's Medical Publishing House, 2021: 325.
[8]	张介宾. 景岳全书[M]. 北京: 人民卫生出版社, 2017: 731.
	ZHANG J B. Jingyue's complete works[M]. Beijing: People's Medical Publishing House, 2017: 731.
[9]	彭思涵, 谢春光, 高泓, 等. 气阴两虚证概述[J]. 四川中医, 2019, 37(10):219-221. DOI: CNKI:SUN:SCZY.0.2019-10-075 .
	PENG S H, XIE C G, GAO H, et al. Summarization of Qi and Yin deficiency[J]. J Sichuan Tradit Chin Med, 2019, 37(10):219-221. DOI: CNKI:SUN:SCZY.0.2019-10-075 .
[10]	关芳, 康雅隆, 谭娟娟, 等. 中药复方专利治疗心肌梗死数据挖掘分析[J]. 中国中医药信息杂志, 2025, 32(3): 58-63. DOI: 10.19879/j.cnki.1005-5304.202408386 .
	GUAN F, KANG Y L, TAN J J, et al. Data mining and analysis of TCM compound patents for treatment of myocardial infarction[J]. Chin J Inf Tradit Chin Med, 2025, 32(3): 58-63. DOI: 10.19879/j.cnki.1005-5304.202408386 .
[11]	李明珠, 陈谦峰, 陶文娟, 等. 基于"膏脂"生理特点与病理变化探析慢性代谢性疾病的防治[J]. 中医杂志, 2022, 63(4):307-311. DOI: 10.13288/j.11-2166/r.2022.04.002 .
	LI M Z, CHEN Q F, TAO W J, et al. Prevention and treatment of chronic metabolic diseases based on the physiological characteristics and pathological changes of "lipids"[J]. J Tradit Chin Med, 2022, 63(4):307-311. DOI: 10.13288/j.11-2166/r.2022.04.002 .
[12]	吴朦, 胡镜清, 江丽杰, 等. 冠心病中医证分类及其关联理化指标的研究[J]. 世界科学技术-中医药现代化, 2016, 18(5):776-785. DOI: 10.11842/wst.2016.05.011 .
	WU M, HU J Q, JIANG L J, et al. Research on the syndrome classification of coronary heart disease and its associated physiochemical indexes[J]. Mod Tradit Chin Med Mater Med World Sci Technol, 2016, 18(5):776-785. DOI: 10.11842/wst.2016.05.011 .
[13]	黄娟, 姚小芹, 熊祎虹, 等. 冠状动脉粥样硬化性心脏病中医证型与冠状动脉造影及血液生化指标相关性研究[J]. 中国中西医结合杂志, 2024, 44(2):149-155. DOI: 10.7661/j.cjim.20230923.249 .
	HUANG J, YAO X Q, XIONG Y H, et al. Correlation of coronary angiography and blood biochemical indexes among different Chinese medicine syndromes of coronary atherosclerotic heart disease[J]. Chin J Integr Tradit West Med, 2024, 44(2):149-155. DOI: 10.7661/j.cjim.20230923.249 .
[14]	张晓囡, 吴敏, 孙长鑫, 等. 基于病证结合探讨动脉粥样硬化瘀毒证的模型构建与评价[J]. 中国实验动物学报, 2023, 31(1):98-105. DOI: 10.3969/j.issn.1005-4847.2023.01.012 .
	ZHANG X N, WU M, SUN C X, et al. Construction and evaluation of a model of atherosclerotic blood-stasis and toxic syndrome based on a combination of disease and syndrome[J]. Acta Lab Anim Sci Sin, 2023, 31(1):98-105. DOI: 10.3969/j.issn.1005-4847.2023.01.012 .
[15]	王文萍, 柴程芝, 寇俊萍, 等. 2种慢性间歇性缺氧小鼠模型模拟气阴两虚证临床指征的比较研究[J]. 中国实验方剂学杂志, 2011, 17(17):171-176. DOI: 10.13422/j.cnki.syfjx.2011.17.087 .
	WANG W P, CHAI C Z, KOU J P, et al. Comparison of clinical indications simulating deficiency of both qi and Yin syndrome induced by two types of chronic intermittent hypoxia in mice[J]. Chin J Exp Tradit Med Formulae, 2011, 17(17):171-176. DOI: 10.13422/j.cnki.syfjx.2011.17.087 .
[16]	中华中医药学会慢性心力衰竭中医诊疗指南项目组. 慢性心力衰竭中医诊疗指南(2022年)[J]. 中医杂志, 2023, 64(7):743-756. DOI: 10.13288/j.11-2166/r.2023.07.016 .
	Project Group of Traditional Chinese Medicine Guideline for Diagnosis and Treatment of Chronic Heart Failure, China Association of Chinese Medicine. Traditional Chinese medicine guideline for diagnosis and treatment of chronic heart failure(2022)[J]. J Tradit Chin Med, 2023, 64(7):743-756. DOI: 10.13288/j.11-2166/r.2023.07.016 .
[17]	钟森杰, 李欣春, 李杰, 等. 心气阴两虚证心力衰竭大鼠血浆代谢组学研究[J]. 湖南中医药大学学报, 2018, 38(9):981-985. DOI: 10.3969/j.issn.1674-070X.2018.09.003 .
	ZHONG S J, LI X C, LI J, et al. Plasma metabonomics of rats with heart failure with heart-qi Yin deficiency syndrome[J]. J Hunan Univ Chin Med, 2018, 38(9):981-985. DOI: 10.3969/j.issn.1674-070X.2018.09.003 .
[18]	顼志兵, 汪卫东, 张莉芬, 等. 冠心病中医证型与血浆vWF、Ps、hs-CRP、FIB、TXB₂、6-keto-PG_1α关系的临床研究[J]. 中国中医基础医学杂志, 2015, 21(1):66-68, 79. DOI: 10.19945/j.cnki.issn.1006-3250.2015.01.028 .
	XU Z B, WANG W D, ZHANG L F, et al. Clinical research on expression of v WF, ps, hs-CRP, FIB, TXB₂, 6-keto-PGF_1α of differential TCM syndrome of coronary heart disease[J]. Chin J Basic Med Tradit Chin Med, 2015, 21(1):66-68, 79. DOI: 10.19945/j.cnki.issn.1006-3250.2015.01.028 .
[19]	田静, 马英杰, 王鹏, 等. 血管内皮功能障碍及其评估技术[J]. 中国比较医学杂志, 2023, 33(7):130-140. DOI: 10.3969/j.issn.1671-7856.2023.07.017 .
	TIAN J, MA Y J, WANG P, et al. Vascular endothelial dysfunction and its functional assessment[J]. Chin J Comp Med, 2023, 33(7):130-140. DOI: 10.3969/j.issn.1671-7856.2023.07.017 .
[20]	王永刚, 钟伟, 于远望, 等. 冠心病中医证型与血脂及冠脉造影所见的相关性分析[J]. 中西医结合心脑血管病杂志, 2013, 11(7):770-771. DOI: 10.3969/j.issn.1672-1349.2013.07.002 .
	WANG Y G, ZHONG W, YU Y W, et al. Correlation of TCM syndrome with blood lipid and coronary angiographic observation in coronary heart disease[J]. Chin J Integr Med Cardio/cerebrovascular Dis, 2013, 11(7):770-771. DOI: 10.3969/j.issn.1672-1349.2013.07.002 .
[21]	王庆盛, 高慧, 夏雨墨, 等. 冠心病气阴两虚证患者中医证候特征及其与生化指标和冠状动脉造影相关性研究进展[J]. 中国中医药信息杂志, 2021, 28(11):142-145. DOI: 10.19879/j.cnki.1005-5304.202101409 .
	WANG Q S, GAO H, XIA Y M, et al. Research progress on TCM syndrome characteristics of coronary heart disease patients with qi Yin deficiency syndrome and its correlation with biochemical indexes and coronary angiography[J]. Chin J Inf Tradit Chin Med, 2021, 28(11):142-145. DOI: 10.19879/j.cnki.1005-5304.202101409 .
[22]	高云霄, 张秋艳, 彭菊琴, 等. 冠心病急性心肌梗死气阴两虚证动物模型的构建与评价[J]. 中国实验方剂学杂志, 2024, 30(4):134-142. DOI: 10.13422/j.cnki.syfjx.20232038 .
	GAO Y X, ZHANG Q Y, PENG J Q, et al. Establishment and evaluation of animal model of acute myocardial infarction with syndrome of Qi and Yin deficiency[J]. Chin J Exp Tradit Med Formulae, 2024, 30(4):134-142. DOI: 10.13422/j.cnki.syfjx.20232038 .
[23]	罗诗雯, 罗权烨, 聂娟, 等. 基于中西医临床病证特点的动脉粥样硬化动物模型分析[J]. 中国比较医学杂志, 2024, 34(8):115-127. DOI: 10.3969/j.issn.1671-7856.2024.08.014 .
	LUO S W, LUO Q Y, NIE J, et al. Animal model analysis of atherosclerosis based on clinical symptoms in traditional Chinese medicine and Western medicine[J]. Chin J Comp Med, 2024, 34(8):115-127. DOI: 10.3969/j.issn.1671-7856.2024.08.014 .
[24]	李欣春, 王菲, 赵振宇, 等. 基于"以方测证"探讨阿霉素诱导慢性心衰小鼠模型中医证候属性与鸢尾素表达特点[J]. 中药药理与临床, 2024, 40(10):74-79. DOI: 10.13412/j.cnki.zyyl.20240912.004 .
	LI X C, WANG F, ZHAO Z Y, et al. Characterization of TCM syndromes and expression of IRISIN in mouse model of doxorubicin-induced chronic heart failure based on syndrome differentiation by formulas[J]. Pharmacol Clin Chin Mater Med, 2024, 40(10):74-79. DOI: 10.13412/j.cnki.zyyl.20240912.004 .
[25]	王文萍, 柴程芝, 寇俊萍, 等. 基于"方证相应"理论验证慢性间歇性缺氧模型气阴两虚证候属性的初步研究[J]. 中国中医基础医学杂志, 2012, 18(1):50-52. DOI: 10.19945/j.cnki.issn.1006-3250.2012.01.025 .
	WANG W P, CHAI C Z, KOU J P, et al. Primary study on verifying the Deficiency of both Qi and Yin Syndrome attribute of chronic intermittent hypoxia mice model based on the theory of "Syndrome Differentiation through Formula Effect Assessment"[J]. Chin J Basic Med Tradit Chin Med, 2012, 18(1):50-52. DOI: 10.19945/j.cnki.issn.1006-3250.2012.01.025 .
[26]	任建勋, 李磊, 尹云泽, 等. 通脉养心丸对小型猪心肌缺血再灌注损伤后期气阴两虚证的干预作用[J]. 中草药, 2018, 49(7):1612-1616. DOI: 10.7501/j.issn.0253-2670.2018.07.019 .
	REN J X, LI L, YIN Y Z, et al. Intervening effects of Tongmai Yangxin Pills on miniature pigs with syndrome of deficiency of both qi and Yin in later stage of myocardial ischemia reperfusion injury[J]. Chin Tradit Herb Drugs, 2018, 49(7):1612-1616. DOI: 10.7501/j.issn.0253-2670.2018.07.019 .
[27]	钟森杰, 李静, 黄淑敏, 等. 慢性心衰心气阴虚证模型大鼠的粪便代谢组学研究[J]. 湖南中医药大学学报, 2020, 40(6):660-665. DOI: 10.3969/j.issn.1674-070X.2020.06.004 .
	ZHONG S J, LI J, HUANG S M, et al. Study on fecal metabolomics of heart-qi Yin deficiency syndrome model rats of chronic heart failure[J]. J Hunan Univ Chin Med, 2020, 40(6):660-665. DOI: 10.3969/j.issn.1674-070X.2020.06.004 .
[28]	李欣春, 梁昊, 胡志希, 等. 高血压心衰大鼠证候本质及药物干预研究[J]. 湖南中医药大学学报, 2018, 38(8):853-857. DOI: 10.3969/j.issn.1674-070X.2018.08.003 .
	LI X C, LIANG H, HU Z X, et al. Study on the essence of syndrome and drug intervention in rats with hypertensive heart failure[J]. J Hunan Univ Chin Med, 2018, 38(8):853-857. DOI: 10.3969/j.issn.1674-070X.2018.08.003 .
[29]	方心怡, 苗润宇, 魏佳华, 等. 现代组学技术在中医证候研究的进展[J]. 中华中医药学刊, 2023, 41(9):132-137. DOI: 10.13193/j.issn.1673-7717.2023.09.027 .
	FANG X Y, MIAO R Y, WEI J H, et al. Progress of modern omics technology in TCM syndromes research[J]. Chin Arch Tradit Chin Med, 2023, 41(9):132-137. DOI: 10.13193/j.issn.1673-7717.2023.09.027 .
[30]	YANG J, TIAN S S, ZHAO J, et al. Exploring the mechanism of TCM formulae in the treatment of different types of coronary heart disease by network pharmacology and machining learning[J]. Pharmacol Res, 2020, 159:105034. DOI:10.1016/j.phrs.2020.105034 .
[31]	GUO R, LUO X L, LIU J J, et al. Omics strategies decipher therapeutic discoveries of traditional Chinese medicine against different diseases at multiple layers molecular-level[J]. Pharmacol Res, 2020, 152:104627. DOI:10.1016/j.phrs. 2020. 104627 .
[32]	韩旭, 李七一, 赖仁胜, 等. ApoE基因多态性与冠状动脉粥样硬化性心脏病中医证型的相关性研究[J]. 中华中医药学刊, 2009, 27(11):2323-2330. DOI: 10.13193/j.archtcm.2009.11.84.hanx.006 .
	HAN X, LI Q Y, LAI R S, et al. Correlative study between apolipoprotein E(ApoE) gene polymorphism and TCM syndrome types of coronary atherosclerotic heart disease(CHD)[J]. Chin Arch Tradit Chin Med, 2009, 27(11):2323-2330. DOI: 10.13193/j.archtcm.2009.11.84.hanx.006 .
[33]	李佳营, 王洋. 人类表型组学在冠心病和中医研究方面的应用[J]. 实用中医内科杂志, 2025, 39(4): 103-107. DOI: 10.13729/j.issn.1671-7813.Z20241105 .
	LI J Y, WANG Y. Application of human phenotypes in coronary heart disease and traditional Chinese medicine research[J]. J Pract Tradit Chin Intern Med, 2025, 39(4): 103-107. DOI: 10.13729/j.issn.1671-7813.Z20241105 .
[34]	周媛, 徐京育, 李启注, 等. 中医药防治冠心病的蛋白质组学研究进展[J]. 世界中医药, 2024, 19(1):109-115. DOI: 10.3969/j.issn.1673-7202.2024.01.021 .
	ZHOU Y, XU J Y, LI Q Z, et al. Advances in the proteomics research on Traditional chinese medicine for the prevention and treatment of coronary heart disease[J]. World Chin Med, 2024, 19(1):109-115. DOI: 10.3969/j.issn.1673-7202.2024.01.021 .
[35]	JIANG Y, ZHAO Q, HUANG S M, et al. Identification and comparison of potential biomarkers by proteomic analysis in traditional Chinese medicine-based heart failure syndromes[J]. Evid Based Complement Alternat Med, 2022, 2022:6338508. DOI:10.1155/2022/6338508 .
[36]	仲东生, 姚魁武, 李娜, 等. 代谢组学在中医药诊疗冠心病研究中的应用进展[J]. 中华中医药杂志, 2024, 39(7):3597-3602.
	ZHONG D S, YAO K W, LI N, et al. Application progress of metabonomics in diagnosis and treatment of coronary heart disease by traditional Chinese medicine[J]. China J Tradit Chin Med Pharm, 2024, 39(7):3597-3602.
[37]	王若冲, 戴宁, 李儒婷, 等. 平台水环境法构建人类疾病动物模型的应用与思考[J]. 中国实验方剂学杂志, 2022, 28(22):205-213. DOI: 10.13422/j.cnki.syfjx.20222293 .
	WANG R C, DAI N, LI R T, et al. Application and thinking of platform method in construction of animal model of human disease[J]. Chin J Exp Tradit Med Form, 2022, 28(22):205-213. DOI: 10.13422/j.cnki.syfjx.20222293 .
[38]	CHAI C Z, KOU J P, ZHU D N, et al. Mice exposed to chronic intermittent hypoxia simulate clinical features of deficiency of both qi and Yin syndrome in traditional Chinese medicine[J]. Evid Based Complement Alternat Med, 2011, 2011:356252. DOI:10.1093/ecam/nep226 .
[39]	莫蔚兰, 庄先飞, 柴程芝, 等. 慢性间歇性缺氧模型与气阴两虚型心脏病的相关性研究[J]. 世界中西医结合杂志, 2014, 9(12):1292-1295. DOI: 10.13935/j.cnki.sjzx.141210 .
	MO W L, ZHUANG X F, CHAI C Z, et al. Correlative study between chronic intermittent hypoxia model and heart disease of Qi and Yin deficiency[J]. World J Integr Tradit West Med, 2014, 9(12):1292-1295. DOI: 10.13935/j.cnki.sjzx.141210 .
[40]	徐一兰, 高杉, 邓兵, 等. 通脉养心丸干预冠心病稳定性心绞痛气阴两虚证的临床生化基础研究[J]. 天津中医药, 2019, 36(1): 18-22.
	XU Y L, GAO S, DENG B, et al. Clinical and biochemical basic research of the qi and Yin deficiency syndrome of coronary heart disease stable angina[J]. Tianjin J Tradit Chin Med, 2019, 36(1): 18-22.
[41]	贺泽龙, 郭振球. 参附液和参麦液对实验性心衰大鼠血流动力学和心肌β受体影响的比较研究[J]. 中国中医基础医学杂志, 2003, 9(5):36-38. DOI: 10.3969/j.issn.1006-3250.2003.05.014 .
	HE Z L, GUO Z Q. Comparing study on effects of Shen-fu and Shen-Mai injection on haemdynamics and myocardial cell β- adrenergic receptor of heart failure in rats[J]. China J Basic Med Tradit Chin Med, 2003, 9(5):36-38. DOI: 10.3969/j.issn.1006-3250.2003.05.014 .
[42]	张秋月, 王保和, 刘伟爽, 等. 益气复脉方对慢性心衰大鼠基质金属蛋白酶活性调节作用的实验研究[J]. 中西医结合心脑血管病杂志, 2016, 14(8):825-829. DOI: 10.3969/j.issn.1672-1349.2016.08.009 .
	ZHANG Q Y, WANG B H, LIU W S, et al. Regulative effects of yiqifumai injection on the activity of matrix metalloproteinase in rats with chronic heart failure[J]. Chin J Integr Med Cardio/Cerebrovascular Dis, 2016, 14(8):825-829. DOI: 10.3969/j.issn.1672-1349.2016.08.009 .
[43]	余怡嫔, 梁昊, 董文波, 等. 三种心力衰竭大鼠模型的病理差异及中医证候评价[J]. 湖南中医药大学学报, 2024, 44(5):737-743. DOI: 10.3969/j.issn.1674-070X.2024.05.004 .
	YU Y P, LIANG H, DONG W B, et al. Pathological differences and Chinese medicine pattern evaluation of three rat models of heart failure[J]. J Hunan Univ Chin Med, 2024, 44(5):737-743. DOI: 10.3969/j.issn.1674-070X.2024.05.004 .
[44]	杨鸣, 王达洋, 龚嫒媛, 等. 气阴两虚型心衰病证结合小鼠模型的构建与评价[J]. 中国中医急症, 2015, 24(12):2076-2078, 2092. DOI: 10.3969/j.issn.1004-745X.2015.12.003 .
	YANG M, WANG D Y, GONG A Y, et al. The construction and evaluation of the Qi and Yin deficiency pattern of hart failure mouse model[J]. J Emerg Tradit Chin Med, 2015, 24(12):2076-2078, 2092. DOI: 10.3969/j.issn.1004-745X.2015.12.003 .
[45]	章忱, 盛学仕, 吕嵘, 等. 动物心肌缺血中医证型规范化标准化研究(二)[J]. 上海中医药杂志, 2003, 37(3):42-45. DOI:10.16305/j.1007-1334.2003.03.018 .
	ZHANG C, SHENG X S, LÜ R, et al. Standardization of Chinese medical syndromes of myocardial ischemia in rats:part two[J]. Shanghai J Tradit Chin Med, 2003, (3):42-45. DOI:10.16305/j.1007-1334.2003.03.018 .
[46]	庄先飞, 柴程芝, 寇俊萍, 等. 气阴两虚证易诱发心血管事件的实验研究[J]. 中国中医基础医学杂志, 2016, 22(1):47-49. DOI: 10.19945/j.cnki.issn.1006-3250.2016.01.021 .
	ZHUANG X F, CHAI C Z, KOU J P, et al. Experimental study on cardiovascular events can be easily induced by the Qi-Yin deficiency syndrome[J]. J Basic Chin Med, 2016, 22(1):47-49. DOI: 10.19945/j.cnki.issn.1006-3250.2016.01.021 .
[47]	蔡小军, 许惠琴, 方泰惠, 等. 生脉饮对气阴两虚型心肌缺血病证结合大鼠模型的研究[J]. 中药药理与临床, 2011, 27(2):3-6. DOI: 10.13412/j.cnki.zyyl.2011.02.002 .
	CAI X J, XU H Q, FANG T H, et al. Counterevidence study on rat models of Qi(气)-Yin(阴) deficiency with acute myocardial ischemia[J]. Pharmacol Clin Chin Mater Med, 2011, 27(2):3-6. DOI: 10.13412/j.cnki.zyyl.2011.02.002 .
[48]	崔钰伟, 臧凝子, 李品, 等. 病证结合动物模型宏观表征指标评价方法研究进展[J]. 中华中医药学刊, 2024, 42(6):195-198. DOI: 10.13193/j.issn.1673-7717.2024.06.040 .
	CUI Y W, ZANG N Z, LI P, et al. Research progress on evaluation methods of macroscopical indicators of disease and syndrome combined with animal models[J]. Chin Arch Tradit Chin Med, 2024, 42(6):195-198. DOI: 10.13193/j.issn.1673-7717.2024.06.040 .
[49]	梁媛, 佘楷杰, 巩子汉, 等. 证候动物模型研制的现状、瓶颈与发展路径探讨[J]. 中国中医基础医学杂志, 2023, 29(3):385-388, 419. DOI: 10.19945/j.cnki.issn.1006-3250.2023.03.006 .
	LIANG Y, SHE K J, GONG Z H, et al. Discussion on current situation, bottleneck and development path of animal model of syndrome[J]. J Basic Chin Med, 2023, 29(3):385-388, 419. DOI: 10.19945/j.cnki.issn.1006-3250.2023.03.006 .