Research Progress on Establishing and Evaluation of Acne Animal Models

doi:10.12300/j.issn.1674-5817.2023.021

Abstract

Abstract:

According to understanding of the pathogenesis of acne, scholars have established animal models of acne inflammation, animal models of grafting human skin acne, and natural acne animal models. The acne inflammation model is mainly induced by bacterial infection, chemical drug application, and foreign matter injection. Natural acne animal models include animals that some are sensitivity to hormones and some have clinical symptoms of acne. It is necessary to select appropriate model animals and replicate model methods for the development of acne intervention products with different degrees and mechanisms. At present, there are only human evaluation standards of acne health functions in China, but no animal evaluation standards, which has affected the in-depth study of the pathogenesis of acne as well as the research and development progress of acne products. This article summarizes the conditions for the occurrence of acne, the characteristics of human skin, the bidirectional effect of Cutibacterium acnes on human skin, acne animal models, and commonly used observation and evaluation indicators, providing the reference for studying the pathogenesis of acne, promoting acne treatment and health care, and developing treatment products.

Key words: Acne, Animal models, Research progress

CLC Number:

R-332

Rui ZHANG, Meiyu LÜ, Jianjun ZHANG, Jinlian LIU, Yan CHEN, Zhiqiang HUANG, Yao LIU, Lanhua ZHOU. Research Progress on Establishing and Evaluation of Acne Animal Models[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 398-405.

Figures/Tables 2

References 45

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模型动物 Model animals	应用范围 Application range	优点 Advantages	缺点 Disadvantages
家兔 Rabbits	对粉刺、角质化异常的疗效评价；痤疮治疗、改善角质化的机制研究	研究最成熟的痤疮模型；兔的毛囊与人类相似；实验操作简单、易成模	兔耳皮肤的优势菌群与人类不同；无法做大量抗痤疮药物的筛选
大鼠 Rats	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	研究较成熟的痤疮模型；炎性反应明显	无法形成黑头粉刺
小鼠 Mice	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	体积小，成本低；炎性反应明显	耳廓注射操作不易；无法形成黑头粉刺；毛囊细胞类型与人类不同
豚鼠 Guinea pigs	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	致炎物质与人类角质细胞的微环境相同	致炎机制与人类痤疮炎性反应不同
裸鼠 Nude mice/rats	抗皮脂腺增生的疗效评价；皮脂代谢的机制研究	皮脂腺分布均匀	成本高；毛囊退化，缺少菌群生长环境；移植后死亡率高
金黄仓鼠 Golden hamsters	抗雄激素、抗皮脂腺增生的疗效评价；激素代谢和皮脂腺代谢的机制研究	可用于建立天然模型，对激素敏感	不易感染，无法做感染和炎性反应研究
犀牛小鼠 Rhino mice	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮	与人类角质不同，无法继发炎性反应；国内研究较少
墨西哥无毛犬 Mexican hairless dogs	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮；可以在身上不同部位筛选外用药	成本高；研究较少
小型猪 Miniature pigs	皮脂腺代谢的机制研究	可用于建立天然模型，与人类皮脂腺的氨基酮戊酸代谢一致	成本高；研究较少

模型动物 Model animals	应用范围 Application range	优点 Advantages	缺点 Disadvantages
家兔 Rabbits	对粉刺、角质化异常的疗效评价；痤疮治疗、改善角质化的机制研究	研究最成熟的痤疮模型；兔的毛囊与人类相似；实验操作简单、易成模	兔耳皮肤的优势菌群与人类不同；无法做大量抗痤疮药物的筛选
大鼠 Rats	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	研究较成熟的痤疮模型；炎性反应明显	无法形成黑头粉刺
小鼠 Mice	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	体积小，成本低；炎性反应明显	耳廓注射操作不易；无法形成黑头粉刺；毛囊细胞类型与人类不同
豚鼠 Guinea pigs	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	致炎物质与人类角质细胞的微环境相同	致炎机制与人类痤疮炎性反应不同
裸鼠 Nude mice/rats	抗皮脂腺增生的疗效评价；皮脂代谢的机制研究	皮脂腺分布均匀	成本高；毛囊退化，缺少菌群生长环境；移植后死亡率高
金黄仓鼠 Golden hamsters	抗雄激素、抗皮脂腺增生的疗效评价；激素代谢和皮脂腺代谢的机制研究	可用于建立天然模型，对激素敏感	不易感染，无法做感染和炎性反应研究
犀牛小鼠 Rhino mice	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮	与人类角质不同，无法继发炎性反应；国内研究较少
墨西哥无毛犬 Mexican hairless dogs	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮；可以在身上不同部位筛选外用药	成本高；研究较少
小型猪 Miniature pigs	皮脂腺代谢的机制研究	可用于建立天然模型，与人类皮脂腺的氨基酮戊酸代谢一致	成本高；研究较少

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