Neurological Dysfunction and Pathological Changes of Rats with

doi:10.3969/j.issn.1674-5817.2020.06.002

Abstract

Abstract: Objective To compare the changes of neurological dysfunction, gross/microscopic pathology and serum inflammatory index of brain tissues after bilateral common carotid artery ligation in different durations. Methods Permanent bilateral common carotid artery ligation was performed in SPF Wistar rats after anesthesia. The successful modeling rats were randomly divided into 3 groups with 10 in each group; while 10 rats in the sham-operated group were treated by only puncture without ligation. The neurological function of each group was tested 2 weeks, 4 weeks, and 6 weeks postoperatively, and then the blood was taken from the abdominal aorta of the anesthetized rats. ELISA method was used to detect the serum levels of interleukin-6 (IL-6), interleukin-1? (IL-1?) and tumor necrosis factor-? (TNF-?). The brain tissue was stained with triphenyltetrazolium chloride (TTC) to evaluate the range of cerebral ischemia. HE staining was used for morphological observation of the brain tissues. Results Compared with the sham-operated group, the neurological function scores of the rats at 2 weeks, 4 weeks, and 6 weeks after bilateral common carotid artery ligation were decreased significantly (P<0.05). Compared with the 2 weeks group, the neurological function scores of the 4 weeks and 6 weeks groups were decreased significantly (P<0.05). Compared with the sham-operated group, the level of IL-6 in the 2 weeks and 4 weeks groups was significantly increased (P<0.05). Compared with the sham-operated group, the ischemic range of brain tissues increased in the 2 weeks, 4 weeks, and 6 weeks groups. Pathological morphology also showed that the brain tissue morphology of rats in the 2 weeks, 4 weeks and 6 weeks groups had different degrees of damage and inflammatory infiltration. Conclusion After ligation of bilateral common arteries, the pathological changes of the brain tissues vary from ischemia to infarction, accompanied with neurobehavioral dysfunction, and the changes are different over time. Thus, the intervention time should be decided according to the mechanism of drug action.

Key words: Bilateral common carotid artery ligation, Nerve dysfunction, Chronic cerebral ischemia, Inflammatory factors, Pathological changes, Rats

CLC Number:

Q95-33
R743

SUN Chengcheng, LIU Jiangang, LIU Meixia, LI Hao, LUO Zenggang. Neurological Dysfunction and Pathological Changes of Rats with[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 470-476.

Figures/Tables 4

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