Laboratory Animal and Comparative Medicine ›› 2016, Vol. 36 ›› Issue (6): 423-427.DOI: 10.3969/j.issn.1674-5817.2016.06.004

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Establishment and Evaluation of Rat Model of Neurogenic Bladder after Spinal Cord Transection Injury

YUAN Xiao-hong1, HE Feng2, JIANG Ze-hui1, ZHAO He1, YE Chao1, WU Shao-ming1, YU Hai-chuan1, LI Chun-gen1   

  1. 1. Second Department of Orthopaedics, Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing 100700, China;
    2. Department of Orthopaedics, The First Hospital Affiliated to Tianjin University of Chinese Medicine, Tianjin 300073, China
  • Received:2016-07-06 Online:2016-12-25 Published:2016-12-25

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Abstract: Objective To establish the ideal animal model of neurogenic bladder (NB) after spinal cord injury (SCI) and evaluate and the state of the bladder. Methods Sixteen female SD rats were divided into control group and the experimental group (T9 spinal cord transection injury model), according to the record of the daily manual urination output to evaluate the NB recovery after SCI. After two weeks the simultaneous recording of the intravesical pressure were used to detect, compare and assess the state of bladders in two groups. Results The urine output of experimental rats at first day, 5th day, 10th day, 13th day, 14th day after operation were significantly higher than thoes of control group rats (P<0.05), and the urine output of experimental rats at 16th day after operation was no statistically significant difference with control group (P>0.05). During a week postoperation, the daily manual urination output increased and reached maximum. One week later postoperation, the daily manual urination output was on the gradually decrease and stable for two weeks. The values of the parameters of the maximum bladder pressure, the basis bladder pressure, voiding threshold, bladder capacity, voiding efficiency in control group and experimental group respectively were 26.60±4.31 mmH2O and 21.66±2.56 mmH2O, 11.66±2.33 mmH2O and 14.72±2.56 mmH2O, 20.46±0.52 mmH2O and 16.99±0.81 mmH2O, 1.36±1.58 min and 2.02±0.36 min, 0.82±0.15 mL and 2.20±0.24 mL, 92.67%±1.97% and 25.33%±4.46%, the differences were statistically significant (P<0.05). Conclusion The NB rat model of T9 level spinal cord transection injury with a strong maneuverability and easy to quantify, repeatable. With the correct postoperative nursing care, there were fewer complications and low mortality. The recording of daily manual urination output and the intravesical pressure can be the good ways to detect the spinal cord recovery and evaluate the status of the bladder.

Key words: Neurogenic bladder (NB), Spinal cord injury (SCI), Animal models, Sprague-Dawley rats, Cystometric

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