Molecular Subtyping Study on Pseudomonas aeruginosa in Rats and Mice with Pulsed Field Gel Electrophoresis

doi:10.3969/j.issn.1674-5817.2016.06.003

Abstract

Abstract: Objective To investigate the correlation between Pseudomonas aeruginosa epidemic strains and host in recent years, Pseudomonas aeruginosa in rat and mice from several faciliies of Shanghai and Jiangsu province were isolated and detected by pulsed field gel electrophoresis (PFGE). Methods The bacteria were isolated from the contents of ileocecal junction under aseptic conditions. The isolated strains were identified by morphological observation and physicochemical test. Extracted genomic DNA of Pseudomonas aeruginosa isolates were detected by PFGE after digested by Xba I restriction enzyme. Results A total of 67 strains were isolated from rats and mice. The genotype of 67 strains was divided into 39 patterns and 14 clusters (A-N). The dominant cluster is I, H, B and G, the rate of these cluster were 17.9%(12/67), 14.9%(10/67), 13.4%(9/67) and 11.9%(8/67) respectively. The isolates from the same source mostly present different gene cluster. Conclusion A great deal of gene polymorphism could be found in all the 67 strains which are diffusively distributed. Obvious prevalent feature could not be found.

Key words: Pseudomonas aeruginosa, Pulsed field gel electrophoresis (PFGE), Molecular subtyping

CLC Number:

Q95-33

FENG Jie, XIE Jian-yun, WEI Xiao-feng, HU Jian-hua, GAO Cheng. Molecular Subtyping Study on Pseudomonas aeruginosa in Rats and Mice with Pulsed Field Gel Electrophoresis[J]. Laboratory Animal and Comparative Medicine, 2016, 36(6): 419-422.

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