Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats

doi:10.3969/j.issn.1674-5817.2014.01.002

Abstract

Abstract: Objective Comparing the characteristics and application of two methods for establishing the hemi-sected spinal cord injury model of rats and provide suitable spinal cord injury models for clinical research. Methods Sixty Sprague-Dawley rats were randomly divided into transverse-hemisected group (A group, n=20), wedge-shaped-block-hemisected group (B group, n=20) and normal control group (C group, n=20). After operation, the stability of the two hemi-sected models were comprehensive assessed by Basso-Beattie-Bresnahan (BBB) scores, inclined plane method, HE staining and MRI detection. Results After operation, the BBB scores and maximum angle of inclined Plane method of rats in group A and group B were significantly lower than group C (P<0.05), but these indexes had no significant difference between group A and group B; In addition, HE staining and MRI detection show a significant morphological change between group A and group B 28 days after operation. Conclusion Both methods of transverse-hemisected and wedge-shaped block-hemisected can establish a stable and reliable spinal cord injury model, and wedge-shaped-block-hemisected model is more suitable to assess graft treatment of spinal cord injury.

Key words: Hemi-sected spinal cord injury, Animal model, Rats

CLC Number:

Q95-33

ZHAO Fu-sheng, ZHANG Xiao-li, WU Geng, CHEN Bin, LI Yue-zhen, JIN Xiu-dong, ZHANG Ji-fei. Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 6-11.

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