Establishment and Preliminary Phenotypic Analysis of Pten Knockout Mouse Model in Spermatogonial Stem Cells

doi:10.3969/j.issn.1674-5817.2014.01.001

Abstract

Abstract: Objective To establish Pten conditional knockout (KO) mouse model and investigate the role and related mechanism of Pten in spermatogonial stem cells(SSCs) and preliminary animal phenotype. Methods Cre/Loxp system was adopted to establish the Pten conditional knockout(KO) mouse model in spermatogonial stem cells. The wild type littermates were used as control and animal phenotype was observed by dissection and Hematoxylin and Eosin (HE) Staining. Then the expression of Plzf, a kind of SSCs marker, was detected by immunofluorescence stain (IF) and the positive cells in IF were counted. Results Pten KO mice shown postnatal developmental retardation and some died around 13 days. No difference in the size of testes was found comparing to the wild type mice, but the result of cell counting indicated that less Plzf positive cells were detected in KO mice (P<0.01). Conclusion Pten conditional knockout mouse model using spermatogonia stem cells is successfully established and Pten might affect SSCs development by regulating the expression of Plzf. This study, to some extent, provides some scientific evidence and new thoughtfor further exploring the complicated regulatory network in SSCs.

Key words: Cre/Loxp system, Pten conditional knockout, Spermatogonia stem cells, Phenotypic analysis, Plzf

CLC Number:

Q95-33

ZHOU Wei, ZHANG Di, YU Zhuo, ZHA Wei-wei, FENG Li-xin. Establishment and Preliminary Phenotypic Analysis of Pten Knockout Mouse Model in Spermatogonial Stem Cells[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 1-5.

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