Advances and Applications in Animal Models of Neuroblastoma

doi:10.12300/j.issn.1674-5817.2022.194

Abstract

Abstract:

Neuroblastoma (NB) is one of the most common malignant solid tumors in children, ranks fourth in the incidence of pediatric tumors, and accounts for 15% of pediatric tumor deaths in children in China. Despite the development of new treatment options, the prognosis for high-risk patients is still poor. An animal model that can replicate the tumorigenesis of NB is an important tool for the prevention and treatment of NB. However, there are currently no animal models that can simulate all features of human NB. To provide a reference for the construction of animal models and treatment of NB, this article introduced several animal models of NB that have been extensively researched: the mouse, chick embryo chorioallantoic membrane, and zebrafish models. At the same time, it elaborated on the species, construction methods, characteristics, advantages and disadvantages, and research progress in NB.

Key words: Neuroblastoma, Animal model, Mice, Chick embryo chorioallantoic-membrane, Zebrafish

CLC Number:

R-332

Zhigang TAN, Jinxin LIU, Chuya ZHENG, Wenfeng LIAO, Luping FENG, Hongli PENG, Xiu YAN, Zhenjian ZHUO. Advances and Applications in Animal Models of Neuroblastoma[J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 288-296.

Figures/Tables 3

References 81

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细胞系 Cell line	小鼠品系 Mouse stain	MYCN基因状态 MYCN status	ALK基因突变 ALK mutation	P53基因突变 P53 mutation	参考文献 Reference
KELLY	BALB/c-nude小鼠	扩增	野生型	野生型	[11]
CHP-212	NSG小鼠	扩增	野生型	野生型	[12]
SKNAS	BALB/c-nude小鼠	非扩增	野生型	H168R	[13]
SH-SY-5Y	Foxn1nu/Nju小鼠、ICR小鼠、BALB/c-nude小鼠	非扩增	F1174L	野生型	[14-16]
IMR-32	NSG小鼠	扩增	野生型	野生型	[17]
IMR-05	SHC小鼠	扩增	野生型	野生型	[18]
LA-N-5	BALB/c-nude小鼠	扩增	R1275Q	野生型	[19]
NB-1	BALB/c-nude小鼠	扩增	野生型扩增	野生型	[20-21]
SK-N-BE(2)	SCID-Beige小鼠	扩增	野生型	C135F	[10]
SK-N-BE(2)-C	BALB/c- nude小鼠、Foxn1nu/Nju小鼠	扩增	野生型	C135F	[11, 22]
CHP-134	NOD-SCID小鼠	扩增	野生型	野生型	[23]
SK-N-DZ	BALB/c-nude小鼠	扩增	野生型	R110L	[24]

细胞系 Cell line	小鼠品系 Mouse stain	MYCN基因状态 MYCN status	ALK基因突变 ALK mutation	P53基因突变 P53 mutation	参考文献 Reference
KELLY	BALB/c-nude小鼠	扩增	野生型	野生型	[11]
CHP-212	NSG小鼠	扩增	野生型	野生型	[12]
SKNAS	BALB/c-nude小鼠	非扩增	野生型	H168R	[13]
SH-SY-5Y	Foxn1nu/Nju小鼠、ICR小鼠、BALB/c-nude小鼠	非扩增	F1174L	野生型	[14-16]
IMR-32	NSG小鼠	扩增	野生型	野生型	[17]
IMR-05	SHC小鼠	扩增	野生型	野生型	[18]
LA-N-5	BALB/c-nude小鼠	扩增	R1275Q	野生型	[19]
NB-1	BALB/c-nude小鼠	扩增	野生型扩增	野生型	[20-21]
SK-N-BE(2)	SCID-Beige小鼠	扩增	野生型	C135F	[10]
SK-N-BE(2)-C	BALB/c- nude小鼠、Foxn1nu/Nju小鼠	扩增	野生型	C135F	[11, 22]
CHP-134	NOD-SCID小鼠	扩增	野生型	野生型	[23]
SK-N-DZ	BALB/c-nude小鼠	扩增	野生型	R110L	[24]

小鼠模型 Mouse model	优势 Advantage	局限性 Limitation	参考文献 Reference
Th-MYCN	代表高危NB型，成瘤率高	成瘤时间长，转移少	[39-40]
LSL-MYCN;dβh-iCre	比Th-MYCN更明确的转基因插入，发病率更高	转移率低	[31]
Th-MYCN/CASP8(KO)	存在转移，成瘤率高	引起原发肿瘤细胞外基质结构的改变	[41]
Th-MYCN/Trp53(KI)	诱导性P53丢失	P53突变多发于复发肿瘤中，小鼠的存活率低	[42]
ALK(F1174)	符合NB表型	临床常见率低	[33]
Th-MYCN/ALK(F1174)	成瘤率高，肿瘤生长快	相关性低	[35]
SV40 Tag	与NB表型一致，肿瘤发病率高，存在转移	所有小鼠在28周龄前死亡	[43]

小鼠模型 Mouse model	优势 Advantage	局限性 Limitation	参考文献 Reference
Th-MYCN	代表高危NB型，成瘤率高	成瘤时间长，转移少	[39-40]
LSL-MYCN;dβh-iCre	比Th-MYCN更明确的转基因插入，发病率更高	转移率低	[31]
Th-MYCN/CASP8(KO)	存在转移，成瘤率高	引起原发肿瘤细胞外基质结构的改变	[41]
Th-MYCN/Trp53(KI)	诱导性P53丢失	P53突变多发于复发肿瘤中，小鼠的存活率低	[42]
ALK(F1174)	符合NB表型	临床常见率低	[33]
Th-MYCN/ALK(F1174)	成瘤率高，肿瘤生长快	相关性低	[35]
SV40 Tag	与NB表型一致，肿瘤发病率高，存在转移	所有小鼠在28周龄前死亡	[43]

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