Effects of Hypoxic Training on Blood EPO, Hb and RBC Level in Rats

doi:10.3969/j.issn.1674-5817.2011.06.005

Abstract

Abstract: Objective To investigate effects of 7 day hypoxic training on the level of Erythropoietin(EPO), hemoglobin(Hb) and red blood cell (RBC) in blood of SD rats, and to obtain the experimental evidence of adaptation to hypoxic training in rats. Methods The rats were exposed in different concentration of hypoxic environment for 2h/day, the time of treadmill running of rats is 30min/day. The level of EPO was checked by ELISA, and the level of RBC and Hb were measured by automatic blood cell analyzer. Results (1) No effects of hypoxic exposure in 15% O₂and 11% O₂ environment on EPO level were found significantly, but treadmill running in 15% O₂ environment enhance EPO level in blood of rats. (2) The number of RBC was increased significantly in rats which exercised on treadmill or stayed in 15% O₂or 11% O₂ environment. (3) The increase of concentration of Hb was found significantly in rats which stayed in 15% O₂or 11% O₂ environment. (4) Effects of hypoxic training on RBC and Hb level disappear in rats 15days after hypoxic training. Conclusion Production of RBC and Hb are significantly induced by hypoxic training in rats, but lasting time of enhancing effect is shorter than 15 days.

Key words: Hypoxia, Erythropoietin, Hemoglobin, Red blood cells, Rats

CLC Number:

Q95-33

LOU Shu-jie, JIAO Ying. Effects of Hypoxic Training on Blood EPO, Hb and RBC Level in Rats[J]. Laboratory Animal and Comparative Medicine, 2011, 31(6): 422-425.

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