Effects of Inagliptin on the Expression of Periosteal and TGF-β1 in Renal Fibrosis of Type 2 Diabetic Rats

doi:10.12300/j.issn.1674-5817.2021.093

Abstract

Abstract:

Objective To analyze the effect of linagliptin on the expression of periostin and transforming growth factor-β1 (TGF-β1) in the process of renal tissue fibrosis in type 2 diabetic rats, and explore the anti-inflammatory and anti-fibrotic protective mechanism of linagliptin on diabetic nephropathy.Methods Totally twenty-four SD rats were injected with streptozotocin in intraperitoneal to replicate the type 2 diabetes rats, and another 12 SD rats were injected with the same dosage of citrate buffer as the control group. The type 2 diabetic rats were randomly divided into two groups: model group and treatment group. The rats in the treatment group were continuously given liggliptin (10 mg?kg^-1?d^-1) for 12 weeks, and the other two groups were given the same amount of normal saline. The pathological changes of renal were observed by HE staining and Masson staining. Immunohistochemistry and RT-PCR were used to detect the expressions of periostin and TGF-β1 protein and mRNA levels in kidney tissues of rats, respectively.Results HE staining showed that compared with the control group, there were a large number of inflammatory cells in the renal stroma in the model group, while there were less inflammatory cells in the treatment group. Messon staining showed that compared with the control group, there were more collagen fibers in renal interstitial in the model group, while the collagen fibers in the treatment group was not obvious. Immunohistochemistry and RT-PCR showed that compared with control group, the protein and mRNA expressions of periostin and TGF-β1 in rat renal in model group were significantly increased (P< 0.05), while they were decreased in treatment group (P< 0.05).Conclusion Inagliptin maybe reduce the protein expression of periostin by inhibiting the overexpression of TGF-β1 in the kidney of type 2 diabetic rats, and slow down the progression of renal fibrosis.

Key words: Inagliptin, Diabetic nephropathy, Renal fibrosis, Periostin, Transforming growth factor-β1, Rats

CLC Number:

Q95-33

Huijie GONG, Guangming ZHANG. Effects of Inagliptin on the Expression of Periosteal and TGF-β1 in Renal Fibrosis of Type 2 Diabetic Rats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 135-140.

Figures/Tables 4

Figure 1 The pathological characteristics in renal interstitium of rats detected by HE staining （×100， ×200）

Figure 2 The pathological characteristics in renal interstitium of rats detected by Masson staining （×100， ×200）

Figure 3 The expressions of periostin and TGF-β1 mRNA of in renal renal interstitium of rats detected by RT-PCR

Figure 4 The expressions of periostin and TGF-β1 protein in renal renal interstitium of rats detected by immunohistochemistry （DAB， ×400）

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