Expression and Significance of Monocarboxylate Transporters in Cortex of Rats After Exercise-induced Fatigue

doi:10.12300/j.issn.1674-5817.2021-053

Abstract

Abstract:

Objective To explore the correlation between the adaptation of the body to exercise-induced fatigue and the expression of monocarboxylate transporters (MCTs) in the cerebral cortex. Methods A total of 42 SD rats were randomly divided into control group and fatigue group 1 to 6. Firstly, the average exhaustive times in each fatigue group were recorded to determine the trend of exercise-induced fatigue after 1, 3, 5, 7, 9 and 14 days exhaustive load treadmill exercises respectively. Subsequently, the changes of MCT1, MCT2 and MCT4 expression in cerebral motor cortex of rat brain were detected by Western blotting and double-labeling immunofluorescence. Results After 5 days of exercise, the average exhaustive time of rats in fatigue of group 3 reached the lowest level [(67.00±7.07) min] and it recovered to (89.17±9.45) min after 9 days of exercise, the difference was statistically significant (P<0.05). After that, the exercise ability of the rats remained basically constant. Western blotting analysis showed a small amount of MCT1, MCT2 and MCT4 normal expression in cerebral motor cortex of rats' brain in control group. After 5 days of exercise, the expression levels of MCT2 in fatigue of group 3 were significantly increased by 74.2% compared with the control group (P<0.05). After 7 days of exercise, the expression levels of MCT1 and MCT4 in fatigue of group 4 were significantly increased by 89.5% and 92.0% compared with the control group (P<0.05). After that, MCT1, MCT2, and MCT4 maintained high expression. The results of immunofluorescence were consistent with those of Western blotting. Conclusion The regulation of cerebral lactic acid metabolism represented by the increased expression of MCTs in the cerebral cortex is related to the adaptation of the body to exercise-induced fatigue, which can be used as a target for exercise-induced fatigue medical intervention.

Key words: Exercise-induced fatigue, Central fatigue, Monocarboxylate transporters, Cerebral cortex, Rats

CLC Number:

G804.7

Chen GAO,Wan WANG,Yurong LI,et al. Expression and Significance of Monocarboxylate Transporters in Cortex of Rats After Exercise-induced Fatigue[J]. Laboratory Animal and Comparative Medicine, 2022, 42(1): 42-47. DOI: 10.12300/j.issn.1674-5817.2021-053.

Figures/Tables 3

Table 1 The exhaustive time of rats in fatigue groups

组别	递增负荷运动天数/d	平均运动力竭时间/min
Fatigue 1	1	120.17±11.18^#△
Fatigue 2	3	81.17±10.57^*
Fatigue 3	5	67.00±7.07^*△
Fatigue 4	7	75.67±4.97^*
Fatigue 5	9	89.17±9.45^*#
Fatigue 6	14	91.17±11.43^*#

Figure 1 Expression of MCT1， MCT2 and MCT4 in cerebral motor cortex of rats in different groups detected by Western blotting

Figure 2 Expression of MCT1， MCT2 and MCT4 in cerebral motor cortex of rats in different groups detected by imunofluorescence（×400）

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