一例瑶山亚种树鼩原发多形性脂肪肉瘤的诊断

doi:10.12300/j.issn.1674-5817.2023.058

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (6): 647-653.DOI: 10.12300/j.issn.1674-5817.2023.058

一例瑶山亚种树鼩原发多形性脂肪肉瘤的诊断

李竹欣¹, 梁亮¹(), 曹颖颖¹, 翟珊珊¹, 戴颖涵¹, 何夏¹, 陶俊宇¹^,², 冷静¹^,²^,³, 唐海波¹^,²()()

^1.广西中医药大学, 南宁 530200
^2.广西高发传染病中西医结合转化医学重点实验室, 南宁 530200
^3.广西特色实验动物模型重点实验室, 南宁 530200

收稿日期:2023-05-06 修回日期:2023-08-08 出版日期:2023-12-25 发布日期:2024-01-06
通讯作者: 唐海波（1983—），男，博士，副研究员，研究方向：抗感染免疫。E-mail： tanghb@gxtcmu.edu.cn。ORCID： 0000-0002-1815-699X
作者简介:李竹欣（1994—），女，硕士研究生，主要从事抗感染免疫研究。E-mail： lizhuxin180@163.com；
梁亮（1982—），女，硕士，工程师，研究方向：抗感染免疫。E-mail： 27344123@qq.com
基金资助:
广西自然科学基金项目“基于树鼩模型探索TLR8在狂犬病发病过程中的作用机理”(2020GXNSFAA297188);广西高等学校千名中青年骨干教师培育计划“广西中医药大学-唐海波”（第三期）(桂教师范（2019）81号);2023年广西中医药多学科交叉创新团队项目“中医药生物新材料关键技术研究与智能装备开发学科交叉创新团队”(GZKJ2303);广西壮族自治区级大学生创新创业训练计划项目“瑶方催乳散对瑶山树鼩催乳效果研究与机制初探”(S202210600080);广西中医药大学校级课题“树鼩GREPT/P15RS基因鉴定及组织中的表达情况研究”(2020MS001)

Diagnosis of a Primary Pleomorphic Liposarcoma in a Tree Shrew（Tupaia belangeri subsp. yaoshanensis）

Zhuxin LI¹, Liang LIANG¹(), Yingying CAO¹, Shanshan ZHAI¹, Yinhan DAI¹, Xia HE¹, Junyu TAO¹^,², Jing LENG¹^,²^,³, Haibo TANG¹^,²()()

^1.Guangxi University of Chinese Medicine, Nanning 530200, China
^2.Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, Nanning 530200, China
^3.Key Laboratory of Characteristic Experimental Animal Models of Guangxi, Nanning 530200, China

Received:2023-05-06 Revised:2023-08-08 Published:2023-12-25 Online:2024-01-06
Contact: TANG Haibo (ORCID: 0000-0002-1815-699X), E-mail: tanghb@gxtcmu.edu.cn

摘要/Abstract

摘要：

在常规饲养5年的老龄雌性瑶山亚种树鼩中发现1例背腹部近髋关节处自发一大肿块。大体观察发现，该瘤体巨大，表面皮毛完整，未发生溃烂；该患病树鼩行动不便，精神状态尚可。异氟烷（2%～4%）吸入麻醉后，采血法安乐死，解剖剥离瘤体，肿瘤边界不清，向周围组织浸润。HE染色结果显示，肿瘤组织可见小灶多形性及大片脂肪母细胞，多形性肉瘤区域主要由异型的上皮样细胞组成，核分裂易见；脂肪母细胞大小显著不同，可见较多多形性脂肪母细胞，细胞体积大，核深染、畸形，边缘见压迹，胞质见多泡状脂滴。免疫组织化学染色结果显示，肿瘤细胞vimentin阳性，小灶多形性脂肪母细胞S-100阳性，Ki-67较高比例阳性。结合HE染色及免疫组织化学结果，综合诊断该例树鼩的自发肿瘤为多形性脂肪肉瘤。

关键词: 瑶山亚种树鼩, 多形性脂肪肉瘤, 诊断, 组织病理学

Abstract:

A large spontaneous mass on the dorsal abdomen near the hip joint was found in an aged female Tupaia belangeri subsp. yaoshanensis after 5 years of routine feeding. The tumor in the diseased tree shrew was huge, with an intact surface and no ulceration; however, it caused inconvenience in movement while maintaining a good mental state. After inhalation anesthesia with isoflurane (2%-4%), the tree shrew was euthanized after the tree shrew entered deep anesthesia. Anatomical dissection of the tumor, the tumor boundary was unclear and infiltrated into surrounding tissues. HE staining showed that small focal pleomorphism and large areas of adipocytes were seen in the tumor tissue. The pleomorphic sarcoma area was mainly composed of atypical epithelioid cells with easily visible nuclear divisions. The size of the adipocytes was significantly different, and more pleomorphic adipocytes were seen. The cell volume was large, the nucleus was deeply stained and deformed, the edge was impressed, and the cytoplasm was seen with multiple vesicular lipid droplets. Immunohistochemical results showed that the tumor cells were positive for Vimentin, the small focal polymorphic adipocyte nucleus was positive for S-100, and Ki-67 exhibited a higher proportion of positivity. Combined with HE staining and immunohistochemical results, the spontaneous tumor in this tree shrew was comprehensively diagnosed as pleomorphic liposarcoma.

Key words: Tupaia belangeri subsp.yaoshanensis, Pleomorphic liposarcoma, Diagnosis, Histopathology

中图分类号:

R738.6

李竹欣, 梁亮, 曹颖颖, 翟珊珊, 戴颖涵, 何夏, 陶俊宇, 冷静, 唐海波. 一例瑶山亚种树鼩原发多形性脂肪肉瘤的诊断[J]. 实验动物与比较医学, 2023, 43(6): 647-653.

Zhuxin LI, Liang LIANG, Yingying CAO, Shanshan ZHAI, Yinhan DAI, Xia HE, Junyu TAO, Jing LENG, Haibo TANG. Diagnosis of a Primary Pleomorphic Liposarcoma in a Tree Shrew（Tupaia belangeri subsp. yaoshanensis）[J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 647-653.

图/表 3

图1 瑶山树鼩整体形态及剖检病变注：A，树鼩整体形态及肿瘤位置（红色箭头所示）；B，瘤体剥离形体。

Figure 1 The overall morphology and autopsy of Tupaia belangeri subsp.yaoshanensisNote：A， Overall morphology and tumor location of tree shrew （as indicated by the red arrow）； B， Exfoliated tumor form.

图2 瑶山树鼩原发肿瘤组织病理学变化（HE染色）注：A，多形性肉瘤区域，上皮样细胞，细胞异型性明显，核仁粗大（红色箭头所示）（×200）。B，脂肪母细胞，细胞大小不一，胞质内有多泡状脂滴（红色箭头所示）；可见多形性脂肪母细胞，细胞体积大，核大，染色质粗颗粒，核仁粗大（×400）。C，脂肪母细胞及多形性脂肪母细胞，多形性脂肪母细胞的细胞核被胞质脂滴空泡挤压，出现压迹（红色箭头所示）（×400）。

Figure 2 Histopathological changes of primary tumor in Tupaia belangeri subsp. yaoshanensis （HE staining）Note：A, Pleomorphic sarcoma region, epithelioid cells, obvious cell atypia, coarse nucleoli (as indicated by the red arrow) (×200). B, Adipocytes have different sizes, and there are many vesicles in the cytoplasm (as indicated by the red arrow). Visible pleomorphic adipocytes display large cell size, large nuclei, coarse granular chromatin and coarse nucleoli (×400). C, Adipocytes and pleomorphic adipocytes. The nuclei of pleomorphic adipocytes are vacuolarly squeezed by cytoplasmic lipid droplets, showing indentation (as indicated by the red arrow) (×400).

图3 瑶山树鼩原发肿瘤的免疫组织化学检测提示多形性脂肪肉瘤（DAB染色）注：A，肿瘤细胞中vimentin弥漫阳性表达（红色箭头所示）（×400）；B，瘤细胞小灶核中Ki-67较高阳性表达（红色箭头所示）（×400）；C和D，瘤细胞小灶核S-100阳性表达（红色箭头所示）（×400）；E，瘤细胞CK阴性表达（×400）； F，肿瘤细胞GCDFP-15阴性表达（×400）；G，肿瘤细胞ER阴性表达（×400）；H，肿瘤细胞GATA阴性表达（×400）。

Figure 3 Immunohistochemical detection of primary tumors suggestive of pleomorphic liposarcoma in the Tupaia belangeri subsp.yaoshanensis （DAB stain）Note：A, Diffuse positive expression of vimentin in tumor cells (as indicated by the red arrow)（×400); B, High expression of Ki-67 in small focal nuclei of tumor cells (as indicated by the red arrow)(×400); C/D, Positive expression of S-100 in small tumor cells (as indicated by the red arrow) (×400); E, Negative expression of cytokeratin (CK) in tumor cells (×400); F, Negative expression of gross cystic disease fluid protein-15 (GCDFP-15) in tumor cells (×400); G, Negative expression of estrogen receptor (ER) in tumor cells (×400); H, Negative expression of GATA Binding Protein 3 (GATA) in tumor cells (×400).

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一例瑶山亚种树鼩原发多形性脂肪肉瘤的诊断

Diagnosis of a Primary Pleomorphic Liposarcoma in a Tree Shrew（Tupaia belangeri subsp. yaoshanensis）

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