束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达及中药干预作用

doi:10.3969/j.issn.1674-5817.2011.02.005

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (2): 96-101.DOI: 10.3969/j.issn.1674-5817.2011.02.005

束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达及中药干预作用

刘瑞敏, 冷晓霞, 陈民利, 潘永明, 寿旗扬, 周卫民, 陶涛, 朱科燕

浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州310053

收稿日期:2010-12-10 出版日期:2011-04-25 发布日期:2011-04-25
作者简介:刘瑞敏, 女, 在读硕士研究生。
基金资助:
1. 浙江省中医“治未病”研究专项(2007WB022); 2. “浙江省卫生高层次创新人才培养工程项目”资助

Expression of c-Fos and Bax in Brain Tissue of Sub-health State Rat Induced by Restraint Stress and Interventional Effect of Traditional Chinese Medicine

LIU Rui-min, LENG Xiao-xia, CHEN Min-li, PAN Yong-ming, SHOU Qi-yang, ZHOU Wei-min, TAO Tao, ZHU Ke-yan

Zhejiang Chinese Medical University Laboratory Animal Research Center, Hangzhou 310053, China

Received:2010-12-10 Online:2011-04-25 Published:2011-04-25

摘要/Abstract

摘要： 目的观察束缚应激致亚健康模型大鼠的脑组织Bax、c-Fos表达情况以及抗衰老片和六味地黄丸的干预作用。方法取雄性SD大鼠20只,体重320~360 g,随机分成4组,即正常对照组、模型对照组、抗衰老片组、六味地黄丸组,每组5只,采用每天限制活动3 h模拟人类束缚应激,建立大鼠亚健康状态模型,造模同时,用抗衰老片、六味地黄丸进行干预。造模14 d结束后,测定大鼠大脑皮质及海马区Bax和c-Fos的表达。结果造模14 d后,模型对照组大鼠脑组织皮质、海马区Bax的表达面积显著增加(P<0.05); 给予抗衰老片和六味地黄丸后,大鼠大脑皮质及海马区Bax表达量明显减少(P<0.05, P<0.01); 模型对照组大鼠脑组织皮质、海马区c-Fos表达量增加; 给予抗衰老片和六味地黄丸后,大鼠的大脑皮质及海马区c-Fos表达有不同程度的减少(P<0.05,P<0.01)。结论束缚应激致亚健康状态的大鼠大脑皮层、海马区Bax、c-Fos表达量增加。抗衰老片和六味地黄丸可减少束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达,预防和减少应激引起的神经元损伤,从而改善亚健康状态。

关键词: 束缚应激, 亚健康状态, 大鼠, 脑组织, Bax, c-Fos, 中药

Abstract: Objective To observe the expression of Bax and c-Fos in brain tissue of sub-health state rat model induced by restraint stress, and further to study the effect of the Traditional Chinese Medicine. Methods Twenty male SD rats of 320-360g, were randomly divided into 4 with 5 rats per group: normal control group, model control group, Anti-ageing tablets group and Liuwei Dihuang pills group, 5 rats per group. The sub-health state animal model was established by limiting daily movement for 3 hours consecutive to 14 days according to the circumstance that can result human physiological stress. At the same time, the rats in Anti-ageing tablets group and Liuwei Dihuang pills group were treated with Anti-ageing tablets and Liuwei Dihuang pills. Respectively during the model building period.The expression level of Bax and c-Fos in brain tissues were detected on completion of the model establishment . Results Fourteen days after molding, in model control group,the expression level of Bax and c-Fos in cerebral cortex and in hippocampus was increased remarkably, while expression of Bax and c-Fos in anti-aging tablets and Liuwei Dihuang pills treated groups was decreased significantly (P<0.05, P<0.01). Conclusion The rat with mental stress were induced by activity restriction have got the expression of Bax and c-Fos significantly increased in brain tissue . The treatments of anti-aging tablets and Liuweidihuang pills improve this kind of mental stress through reducing the expression of Bax and c-Fos, protecting and decreasing neuron injuries caused by stressors.

Key words: Restraint stress, Sub-health state, Rat, Brain tissue, Bax, c-Fos, Traditional Chinese Medicine

中图分类号:

Q95-33

刘瑞敏, 冷晓霞, 陈民利, 潘永明, 寿旗扬, 周卫民, 陶涛, 朱科燕. 束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达及中药干预作用[J]. 实验动物与比较医学, 2011, 31(2): 96-101.

LIU Rui-min, LENG Xiao-xia, CHEN Min-li, PAN Yong-ming, SHOU Qi-yang, ZHOU Wei-min, TAO Tao, ZHU Ke-yan. Expression of c-Fos and Bax in Brain Tissue of Sub-health State Rat Induced by Restraint Stress and Interventional Effect of Traditional Chinese Medicine[J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 96-101.

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束缚应激致亚健康状态大鼠脑组织Bax、c-Fos的表达及中药干预作用

Expression of c-Fos and Bax in Brain Tissue of Sub-health State Rat Induced by Restraint Stress and Interventional Effect of Traditional Chinese Medicine

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