常用近交系大鼠微卫星遗传标记筛选及分析

doi:10.12300/j.issn.1674-5817.2025.109

摘要/Abstract

摘要：

目的筛选一套覆盖大鼠1-20号常染色体和X染色体、且每条染色体含2-4个的STR（Short Tandem Repeats， STRs）标记，建立适用于F344、BN、DA、Lewis、PVG这5个常用近交系大鼠的遗传背景检测、遗传污染检测及品系间鉴定的专用标记组合。方法每个品系抽样6只，雌雄各半，抽提基因组DNA。从国标、文献中分别选取27个和34个大鼠STR标记，共计61个。采用单重STR PCR扩增结合毛细管电泳技术对5个近交系大鼠样本进行分型，利用GeneMapper ID v3.2软件分析STR基因型数据。基于STR分型结果，通过GenAlEx 6.51b2软件计算品系间遗传距离，并借助MEGA7软件构建近交系大鼠遗传进化树。结果在61个STR标记中，D15mit3存在非特异扩增，D3wox7无特异性扩增产物，最终可用的STR标记共计59个。该59个STR标记在5个近交系大鼠品系中特异性扩增成功率达100%，且每个样本的分型结果均为纯合，5个近交系大鼠的平均纯合度达100%，符合近交系遗传质量控制的要求。遗传进化树显示，BN与F344的STR标记等位基因差异数为49，遗传距离达1.775，为所有品系对中的最大值，表明二者遗传分化程度最高、亲缘关系最远；F344、DA、PVG品系聚为一支，表明三者起源于同一祖先；BN和Lewis品系聚为一支，表明二者起源于同一祖先，5个品系的遗传亲缘关系与既往报道基本一致。在59个STR分型结果中，LCA、AGT、D5Hmgc2在5个品系间无多态性，其余 56个STR标记具有品系间多态性，且覆盖大鼠20条常染色体和X染色体（每条染色体有2-4个STR标记），因此可作为一套近交系大鼠遗传背景检测的STR标记组合。从56个STR标记中选取42个STR标记可用于近交系大鼠遗传污染检测。D7wox14、D15rat123、D20wox3在5个品系中的扩增产物长度存在品系间差异，可用于5个常用近交系大鼠品系间快速鉴定。结论本研究成功筛选出了一套可用于5个常用近交系大鼠的遗传检测STR标记，并建立了分别用于遗传污染检测与品系间鉴定的专用标记组合。

关键词: 近交系大鼠, 微卫星标记, 遗传检测, 进化树, 筛选

Abstract:

Objective To screen a set of Short Tandem Repeats(STR) markers covering rat chromosomes 1-20 (autosomes) and the X chromosome (2-4 markers per chromosome), and establish a dedicated marker panel for genetic background detection, genetic contamination detection, and inter-strain identification of five commonly used inbred rat strains (F344, BN, DA, Lewis, and PVG). Methods 6 samples were collected from each strain (half male and half female). Genomic DNA was extracted from each sample. A total of 61 STR markers were selected, including 27 from national standards and 34 from reference. Singleplex STR PCR amplification combined with capillary electrophoresis was used for genotyping of the five inbred rat strains, and genotype data were analyzed using GeneMapper ID v3.2 software. Based on the STR genotyping results, genetic distances between strains were calculated with GenAlEx 6.51b2 software, and a phylogenetic tree of the inbred rat strains was constructed using MEGA7 software. Results Among the 61 STR markers, non-specific amplification was observed for D15mit3, while no specific amplification product was obtained for D3wox7, resulting in 59 usable STR markers. The success rate of specific amplification for these 59 markers reached 100% across 5 inbred strains. Additionaly, the genotyping results of each sample were homozygous, and the average homozygosity of the 5 inbred strains reached 100%, which meets the requirements for genetic quality control of inbred strains. The phylogenetic tree showed that the number of STR allelic differences between BN and F344 strains was 49, with a genetic distance of 1.775, both of which were the highest among all strain pairs. This indicated that the two strains exhibited the highest level of genetic differentiation and the farthest genetic relationship. In addition, F344, DA, and PVG clustered into one clade, suggesting that they originated from a common ancestor, while BN and Lewis formed another clade, implying a shared ancestral origin. The genetic relationships among the 5 strains were largely consistent with previous reports. Among the genotyping results of the 59 STR markers, LCA, AGT, and D5Hmgc2 exhibited no inter-strain polymorphism across the 5 inbred strains, while the remaining 56 markers possessed inter-strain polymorphism. These 56 markers covered rat 20 autosomes and the X chromosome (2-4 markers per chromosome). Thus, these 56 markers can serve as a panel for genetic background detection of inbred rat strains. A subset of 42 markers selected from the 56 polymorphic ones can be employed for genetic contamination detection. Additionally, The amplified product lengths of D7wox14, D15rat123 and D20wox3 exhibited inter-strain differences among the 5 strains, which can be used for the rapid identification of 5 commonly used inbred rat strains. Conclusion This study successfully screened out of a set of STR markers for genetic testing of 5 commonly used inbred rat strains, and established dedicated marker panels for genetic contamination detection and inter-strain identification, respectively.

Key words: Inbred rats, Short tandem repeats, STRs, Genetic detection, Evolutionary tree, Screen

中图分类号:

Q95-33

汤建平,赵丽亚,赵莹.常用近交系大鼠微卫星遗传标记筛选及分析[J]. 实验动物与比较医学.. DOI: 10.12300/j.issn.1674-5817.2025.109.

TANG Jianping,ZHAO Liya,ZHAO Ying. Screening and Analysis of Microsatellite Genetic Markers in Commonly Used Inbred Rat Strains[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2025.109.

图/表 6

参考文献 23

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标记Marker	染色体Chromosome	物理位置/bp^# Physical position/bp	正向引物序列（5’→3‘）Upstream primer sequence（5’→3‘）	反向引物序列（5’→3‘）Downstream primer sequence（5’→3‘）	退火温度/℃ Annealing temperature/℃
D1wox18	chr1	100,133,276	TCACTCTCATTAAACTAGGAATGC	ACTGTTGGGTAACAAAGTTATGG	53
D1rat345*	chr1	112,693,166	TTCACACAAATGCCACCAGT	CAAAGAGATAGGGCGACAGG	59
D1wox21	chr1	198,655,873	GACATGGTGAGTGAAGACCC	TGAGGCTATCCTGAGCTAACTC	59
D1mgh14*	chr1	278,228,767	CCGCACTGAGCTCTCAGAG	CCCAACCATTGAGCTAGTAAGG	61
D2rat3	chr2	8,579,280	TCTCTGGTGGATACCTCACAAA	ACATGATGTGGAATTCCCCC	59
D2mgh26*	chr2	105,075,219	ATTGGTGATCAGATTTTTTTCTCC	CAGTTGAAAATTCCTGTATCTGG	57
D2wox15*	chr2	188,450,000	GGTGCTAGTAGACAATAAGATAGAT	TTCATGAGTTTTCACTGTTTGC	53
D2Arb20	chr2	200,585,161	ACTAGAGTCAGGAGGCAAAACC	ACCCTATGTGAAATGGTGATGC	59
D3wox9*	chr3	51,821,710	TTCAGGTATGATTCGGGAAC	ATTGGCGATATCATTTCACTAAC	59
RH94469	chr3	93,380,189	CTTATGTTACCTCACAGCCTGG	GGGTGGGCCATCTTTATAATC	61
D4wox27*	chr4	3,044,017	GGCCTTCATTTGTCCTTTGA	CACCATTATGCCCAGCCTTA	59
D4Arb10*	chr4	61,708,051	ACTCATCTTCTGAGGAGTAGCG	TTCACAGTCTATTTGGTAGGGC	59
D4mit15*	chr4	111,809,356	CCTAATGACAGCAATAGAAACTACG	TTTTGGTACTGGAGAAAGTTGG	61
D5mit18	chr5	17,062,312	GCAAGACAGGCTTCCACAAT	AATACTGCCGGAGCAGAAAA	59
D5Hmgc2*	chr5	78,041,475	ATGTTCCCTGATGTCCCTTC	AAACCATGCCACGTGAAC	53
D5wox11	chr5	138,180,823	CTTAGGTCAGCTGGTCCCTAG	CATGGTGCTTTTCCTTGTTG	55
D6rat105	chr6	19,888,103	GCATGAGGCAGAGCCAATTA	GCCAGTTTGCCTTCACTTTC	59
D6mit1*	chr6	99,273,921	TTAGGAGAGAACTGAAAGTTGTCC	ATGGTGCACTATGGTGGTCA	61
D6wox12	chr6	138,065,848	CTTCCCTACCTTCTACAACACTAAG	TAAACCAAAGAGCATGATGAAG	61
D7wox14	chr7	102,588,256	GGGAGTAAAAGAGTGCATTCC	TACCCCAATCCTGAACCAC	61
D7wox24	chr7	115,250,056	AGCCTCAATCTGGCCTAGCTC	TGACTCTTGACAATGGCACA	59
D7mgh3*	chr7	123,632,617	CGGCATGTGTCTCTGTGTG	TGACTTCTGGTGTCCTCACG	59
D8wox4*	chr8	50,529,563	GATTTGAAGCGATTGTCCAT	GTCTAGCTGCCCACAGGAG	57
D8rat14*	chr8	108,068,142	GCCCATACGTTGCATCAAGT	GGCCGGTCTAATTATTTCTTCA	59
D8wox11	chr8	116,873,553	AGTGGGAATGCTTAGCCAAA	CAGGCCTTTACACCTTCCTG	57
D9mit2*	chr9	71,771,288	TGACCAGTTAGCCCTTTCCA	GGGAGCAGGGTTCTACACAG	59
D9rat110	chr9	102,531,710	TGGGTGCTTAATTTTGTTTGTTT	GCTCCTAACCCATCCAACTTT	55
D10rat199	chr10	1,381,054	TGCCAAATAATGATTGTTCCTTC	CCTCCTCTGGCTTCTTGAGA	55
D10wox12	chr10	56,220,298	CGCTGGGAGGAAAGAGAG	AGGCTTGCACTCTGTGTTTG	59
D10mit12*	chr10	94,240,320	ATGGGAGGGAACAAGTCTTC	GAGGGAGAGAGAAAGAGAGACAG	61
D11wox3*	chr11	60,397,957	CACTTTGAGGCCATTCTGAA	CCTTCTCTTTGTGAAAAATAAAGTC	59
D11mgh3*	chr11	69,649,708	GGAGCTGAAATACGAGAGAAATAA	GTCCTGCTGGCTGTGCAT	59
D11wox2	chr11	80,357,513	TTTCCAGGTGCCAAATGTAG	TATATTGTGACAAAAGAAAGGCAC	55
D12mit2*	chr12	22,650,702	GTGGCTCTTTTCCTTAGGGA	TCGGCTTCTGAATGTATTGG	55
D12rat36	chr12	37,521,884	TGTAGAGAATCTAAAGCCCTCTTG	GAGGGACTTGGGAAGAGTGTT	61
D13rat60	chr13	26,919,274	ACGGCACAATCAATTGATGA	AAACTCACATGCTTGCACGT	61
LCA*	chr13	52,147,717	TACAGAGCAAGCTCCAGGAC	TGTTTCTAATCCATAGGAAGTGC	59
D13rat131	chr13	83,263,753	CAGAATCTTGTGCCTCCTGA	TATCGACTTGCCATTGTTCG	55
D14rat72	chr14	8,058,678	CAGCAACACACACACCATACA	AGGAAGTCACACCACAAGGG	61
ALB*	chr14	19,191,541	TTTTCGTAGTAACGGAAGCC	TAAGGATTCTCAGATGCAAATG	59
D14wox14	chr14	22,009,849	ACTTAATTACACACACAAACACAGA	CTTTGCTTTCTTTTAGCCATTT	61
D14rat39	chr14	77,239,784	GGCTGATCCCCAAGTGTCTA	TGAATCAAAAGGGGCATTTC	59
D15rat55	chr15	211,024	TATCCTCTTCTGCCCTCCAA	CCCACCCAGATGTGTTCTCT	59
D15rat123	chr15	59,651,151	CCCCTACATCCATGGCATAC	TTCCCCCTCTTGTTCTTCCT	55
D16wox9*	chr16	18,740,885	ACCTACACGGACACATGGTG	GTTGTACTTCCTGATTTCCCTTT	61
D16rat15	chr16	80,316,290	ATCGGTAAACAAATCGCTGG	TGGCATCTTTCGCTTAGACA	57
D17rat78	chr17	42,541,872	TGCATGAGTCAATCTTCAATCA	CAGACTTCCTATTTTCCTATCTGCA	57
D17wox12*	chr17	63,994,211	AGGAAATTAAGAGAAGTTGGGACT	TATGCTCTTTGGGCAGCTTA	55
D18wox7	chr18	15,539,551	GTGGAAAGCCTTCTGTTCAA	AGAATTCAATAATAACAGTCCCACT	61
TILP*	chr18	38,177,441	AATCACTGGATGCTGGAAGA	AGGGAGCAGAACTACTAAAGATACA	57
D18rat116	chr18	40,057,808	TGCATATCCTTTTGCAACCA	TCCACTGTTCCAGGGAAGAC	55
D18rat8	chr18	71,569,072	GCGGCTGGAGTTATGACTTT	GCCCACTCCTGTGTGAGATT	61
D19wox8	chr19	24,455,726	TGCCCGTCTCTGTTACTCAT	CAAGAACCCTGAGGCAATAA	59
D19wox9	chr19	42,099,630	GTACAGGAACACTGCCCTTG	GGAATGTGTATGTGTGATGACG	55
AGT*	chr19	69,426,540	TATGTAACTCAACGCCAGCC	GAAGCCCTAGTGGCAGATG	59
D20wox3*	chr20	4,855,475	AGGAAATGGGTTTCAGTTCC	CAGGATTCTGTGGCAATCCG	57
D20mit4	chr20	36,923,763	TAAATTCAGAGTGTTGTTCCTGC	CACACCCAATGAATGCTCTG	59
DXwox6*	chrX	28,435,436	GTTTTCCCGCTTCACCAG	AGAAGGAGAAAGCGACCG	59
DXmgh9	chrX	70,352,120	GTTGTCTGGTGCCAAAAGGT	AAGGATGATCTTAAGCTCCTGG	59

标记Marker	染色体Chromosome	物理位置/bp^# Physical position/bp	正向引物序列（5’→3‘）Upstream primer sequence（5’→3‘）	反向引物序列（5’→3‘）Downstream primer sequence（5’→3‘）	退火温度/℃ Annealing temperature/℃
D1wox18	chr1	100,133,276	TCACTCTCATTAAACTAGGAATGC	ACTGTTGGGTAACAAAGTTATGG	53
D1rat345*	chr1	112,693,166	TTCACACAAATGCCACCAGT	CAAAGAGATAGGGCGACAGG	59
D1wox21	chr1	198,655,873	GACATGGTGAGTGAAGACCC	TGAGGCTATCCTGAGCTAACTC	59
D1mgh14*	chr1	278,228,767	CCGCACTGAGCTCTCAGAG	CCCAACCATTGAGCTAGTAAGG	61
D2rat3	chr2	8,579,280	TCTCTGGTGGATACCTCACAAA	ACATGATGTGGAATTCCCCC	59
D2mgh26*	chr2	105,075,219	ATTGGTGATCAGATTTTTTTCTCC	CAGTTGAAAATTCCTGTATCTGG	57
D2wox15*	chr2	188,450,000	GGTGCTAGTAGACAATAAGATAGAT	TTCATGAGTTTTCACTGTTTGC	53
D2Arb20	chr2	200,585,161	ACTAGAGTCAGGAGGCAAAACC	ACCCTATGTGAAATGGTGATGC	59
D3wox9*	chr3	51,821,710	TTCAGGTATGATTCGGGAAC	ATTGGCGATATCATTTCACTAAC	59
RH94469	chr3	93,380,189	CTTATGTTACCTCACAGCCTGG	GGGTGGGCCATCTTTATAATC	61
D4wox27*	chr4	3,044,017	GGCCTTCATTTGTCCTTTGA	CACCATTATGCCCAGCCTTA	59
D4Arb10*	chr4	61,708,051	ACTCATCTTCTGAGGAGTAGCG	TTCACAGTCTATTTGGTAGGGC	59
D4mit15*	chr4	111,809,356	CCTAATGACAGCAATAGAAACTACG	TTTTGGTACTGGAGAAAGTTGG	61
D5mit18	chr5	17,062,312	GCAAGACAGGCTTCCACAAT	AATACTGCCGGAGCAGAAAA	59
D5Hmgc2*	chr5	78,041,475	ATGTTCCCTGATGTCCCTTC	AAACCATGCCACGTGAAC	53
D5wox11	chr5	138,180,823	CTTAGGTCAGCTGGTCCCTAG	CATGGTGCTTTTCCTTGTTG	55
D6rat105	chr6	19,888,103	GCATGAGGCAGAGCCAATTA	GCCAGTTTGCCTTCACTTTC	59
D6mit1*	chr6	99,273,921	TTAGGAGAGAACTGAAAGTTGTCC	ATGGTGCACTATGGTGGTCA	61
D6wox12	chr6	138,065,848	CTTCCCTACCTTCTACAACACTAAG	TAAACCAAAGAGCATGATGAAG	61
D7wox14	chr7	102,588,256	GGGAGTAAAAGAGTGCATTCC	TACCCCAATCCTGAACCAC	61
D7wox24	chr7	115,250,056	AGCCTCAATCTGGCCTAGCTC	TGACTCTTGACAATGGCACA	59
D7mgh3*	chr7	123,632,617	CGGCATGTGTCTCTGTGTG	TGACTTCTGGTGTCCTCACG	59
D8wox4*	chr8	50,529,563	GATTTGAAGCGATTGTCCAT	GTCTAGCTGCCCACAGGAG	57
D8rat14*	chr8	108,068,142	GCCCATACGTTGCATCAAGT	GGCCGGTCTAATTATTTCTTCA	59
D8wox11	chr8	116,873,553	AGTGGGAATGCTTAGCCAAA	CAGGCCTTTACACCTTCCTG	57
D9mit2*	chr9	71,771,288	TGACCAGTTAGCCCTTTCCA	GGGAGCAGGGTTCTACACAG	59
D9rat110	chr9	102,531,710	TGGGTGCTTAATTTTGTTTGTTT	GCTCCTAACCCATCCAACTTT	55
D10rat199	chr10	1,381,054	TGCCAAATAATGATTGTTCCTTC	CCTCCTCTGGCTTCTTGAGA	55
D10wox12	chr10	56,220,298	CGCTGGGAGGAAAGAGAG	AGGCTTGCACTCTGTGTTTG	59
D10mit12*	chr10	94,240,320	ATGGGAGGGAACAAGTCTTC	GAGGGAGAGAGAAAGAGAGACAG	61
D11wox3*	chr11	60,397,957	CACTTTGAGGCCATTCTGAA	CCTTCTCTTTGTGAAAAATAAAGTC	59
D11mgh3*	chr11	69,649,708	GGAGCTGAAATACGAGAGAAATAA	GTCCTGCTGGCTGTGCAT	59
D11wox2	chr11	80,357,513	TTTCCAGGTGCCAAATGTAG	TATATTGTGACAAAAGAAAGGCAC	55
D12mit2*	chr12	22,650,702	GTGGCTCTTTTCCTTAGGGA	TCGGCTTCTGAATGTATTGG	55
D12rat36	chr12	37,521,884	TGTAGAGAATCTAAAGCCCTCTTG	GAGGGACTTGGGAAGAGTGTT	61
D13rat60	chr13	26,919,274	ACGGCACAATCAATTGATGA	AAACTCACATGCTTGCACGT	61
LCA*	chr13	52,147,717	TACAGAGCAAGCTCCAGGAC	TGTTTCTAATCCATAGGAAGTGC	59
D13rat131	chr13	83,263,753	CAGAATCTTGTGCCTCCTGA	TATCGACTTGCCATTGTTCG	55
D14rat72	chr14	8,058,678	CAGCAACACACACACCATACA	AGGAAGTCACACCACAAGGG	61
ALB*	chr14	19,191,541	TTTTCGTAGTAACGGAAGCC	TAAGGATTCTCAGATGCAAATG	59
D14wox14	chr14	22,009,849	ACTTAATTACACACACAAACACAGA	CTTTGCTTTCTTTTAGCCATTT	61
D14rat39	chr14	77,239,784	GGCTGATCCCCAAGTGTCTA	TGAATCAAAAGGGGCATTTC	59
D15rat55	chr15	211,024	TATCCTCTTCTGCCCTCCAA	CCCACCCAGATGTGTTCTCT	59
D15rat123	chr15	59,651,151	CCCCTACATCCATGGCATAC	TTCCCCCTCTTGTTCTTCCT	55
D16wox9*	chr16	18,740,885	ACCTACACGGACACATGGTG	GTTGTACTTCCTGATTTCCCTTT	61
D16rat15	chr16	80,316,290	ATCGGTAAACAAATCGCTGG	TGGCATCTTTCGCTTAGACA	57
D17rat78	chr17	42,541,872	TGCATGAGTCAATCTTCAATCA	CAGACTTCCTATTTTCCTATCTGCA	57
D17wox12*	chr17	63,994,211	AGGAAATTAAGAGAAGTTGGGACT	TATGCTCTTTGGGCAGCTTA	55
D18wox7	chr18	15,539,551	GTGGAAAGCCTTCTGTTCAA	AGAATTCAATAATAACAGTCCCACT	61
TILP*	chr18	38,177,441	AATCACTGGATGCTGGAAGA	AGGGAGCAGAACTACTAAAGATACA	57
D18rat116	chr18	40,057,808	TGCATATCCTTTTGCAACCA	TCCACTGTTCCAGGGAAGAC	55
D18rat8	chr18	71,569,072	GCGGCTGGAGTTATGACTTT	GCCCACTCCTGTGTGAGATT	61
D19wox8	chr19	24,455,726	TGCCCGTCTCTGTTACTCAT	CAAGAACCCTGAGGCAATAA	59
D19wox9	chr19	42,099,630	GTACAGGAACACTGCCCTTG	GGAATGTGTATGTGTGATGACG	55
AGT*	chr19	69,426,540	TATGTAACTCAACGCCAGCC	GAAGCCCTAGTGGCAGATG	59
D20wox3*	chr20	4,855,475	AGGAAATGGGTTTCAGTTCC	CAGGATTCTGTGGCAATCCG	57
D20mit4	chr20	36,923,763	TAAATTCAGAGTGTTGTTCCTGC	CACACCCAATGAATGCTCTG	59
DXwox6*	chrX	28,435,436	GTTTTCCCGCTTCACCAG	AGAAGGAGAAAGCGACCG	59
DXmgh9	chrX	70,352,120	GTTGTCTGGTGCCAAAAGGT	AAGGATGATCTTAAGCTCCTGG	59

标记 Marker	染色体 Chromosome	物理位置/bp^# Physical position /bp	各品系的产物长度/bp Product size of each strain/bp					多态性数量(Number of polymorphisms)
标记 Marker	染色体 Chromosome	物理位置/bp^# Physical position /bp	F344	BN	DA	Lewis	PVG	多态性数量(Number of polymorphisms)
D1wox18	chr1	100,133,276	122	120	122	120	118	3
D1rat345*	chr1	112,693,166	125	125	121	125	129	3
D1wox21	chr1	198,655,873	85	97	73	95	97	4
D1mgh14*	chr1	278,228,767	142	120	150	150	140	4
D2rat3	chr2	8,579,280	126	122	124	117	122	4
D2mgh26*	chr2	105,075,219	126	132	134	134	126	3
D2wox15*	chr2	188,450,000	151	131	133	133	145	4
D2Arb20	chr2	200,585,161	231	235	207	215	207	4
D3wox9*	chr3	51,821,710	117	123	115	121	121	4
RH94469	chr3	93,380,189	239	239	239	227	221	3
D4wox27*	chr4	3,044,017	214	197	219	197	214	3
D4Arb10*	chr4	61,708,051	389	392	389	389	409	3
D4mit15*	chr4	111,809,356	215	215	215	215	202	2
D5mit18	chr5	17,062,312	235	219	225	235	231	4
D5wox11	chr5	138,180,823	137	123	137	137	137	2
D6rat105	chr6	19,888,103	229	225	204	227	227	4
D6mit1*	chr6	99,273,921	134	122	112	134	134	3
D6wox12	chr6	138,065,848	127	127	135	127	127	2
D7wox14	chr7	102,588,256	120	126	122	118	124	5
D7wox24	chr7	115,250,056	133	133	133	133	129	2
D7mgh3*	chr7	123,632,617	233	243	233	243	233	2
D8wox4*	chr8	50,529,563	131	119	129	131	117	4
D8rat14*	chr8	108,068,142	169	161	161	161	169	2
D8wox11	chr8	116,873,553	183	191	181	191	183	3
D9mit2*	chr9	71,771,288	185	185	190	190	185	2
D9rat110	chr9	102,531,710	146	158	169	158	146	3
D10rat199	chr10	1,381,054	190	188	188	188	188	2
D10wox12	chr10	56,220,298	95	95	95	91	91	2
D10mit12*	chr10	94,240,320	142	144	144	144	144	2
D11wox3*	chr11	60,397,957	189	183	183	185	181	4
D11mgh3*	chr11	69,649,708	150	166	186	145	145	4
D11wox2	chr11	80,357,513	152	146	148	146	148	3
D12mit2*	chr12	22,650,702	187	215	187	206	196	4
D12rat36	chr12	37,521,884	205	202	205	188	190	4
D13rat60	chr13	26,919,274	131	125	125	127	127	3
D13rat131	chr13	83,263,753	149	151	149	134	134	3
D14rat72	chr14	8,058,678	152	181	162	179	152	4
ALB*	chr14	19,191,541	136	120	136	133	136	3
D14wox14	chr14	22,009,849	122	112	122	108	108	3
D14rat39	chr14	77,239,784	240	248	240	248	240	2
D15rat55	chr 15	211,024	210	228	228	228	228	2
D15rat123	chr15	59,651,151	136	132	130	122	120	5
D16wox9*	chr16	18,740,885	145	137	191	145	145	3
D16rat15	chr16	80,316,290	142	133	144	133	144	3
D17rat78	chr17	42,541,872	151	157	155	155	155	3
D17wox12*	chr17	63,994,211	136	132	115	119	115	4
D18wox7	chr18	15,539,551	148	142	189	193	189	4
TILP*	chr18	38,177,441	262	240	262	242	242	3
D18rat116	chr18	40,057,808	219	223	219	223	225	3
D18rat8	chr18	71,569,072	155	165	157	165	161	4
D19wox8	chr19	24,455,726	124	126	111	124	111	3
D19wox9	chr19	42,099,630	154	158	158	156	160	4
D20wox3*	chr20	4,855,475	116	110	120	128	114	5
D20mit4	chr20	36,923,763	215	201	192	198	215	4
Dxwox6*	chrX	28,435,436	171	138	173	138	175	4
DXmgh9	chrX	70,352,120	120	146	146	146	120	2
纯合度 homozygosity	/	/	100%	100%	100%	100%	100%	/

标记 Marker	染色体 Chromosome	物理位置/bp^# Physical position /bp	各品系的产物长度/bp Product size of each strain/bp					多态性数量(Number of polymorphisms)
标记 Marker	染色体 Chromosome	物理位置/bp^# Physical position /bp	F344	BN	DA	Lewis	PVG	多态性数量(Number of polymorphisms)
D1wox18	chr1	100,133,276	122	120	122	120	118	3
D1rat345*	chr1	112,693,166	125	125	121	125	129	3
D1wox21	chr1	198,655,873	85	97	73	95	97	4
D1mgh14*	chr1	278,228,767	142	120	150	150	140	4
D2rat3	chr2	8,579,280	126	122	124	117	122	4
D2mgh26*	chr2	105,075,219	126	132	134	134	126	3
D2wox15*	chr2	188,450,000	151	131	133	133	145	4
D2Arb20	chr2	200,585,161	231	235	207	215	207	4
D3wox9*	chr3	51,821,710	117	123	115	121	121	4
RH94469	chr3	93,380,189	239	239	239	227	221	3
D4wox27*	chr4	3,044,017	214	197	219	197	214	3
D4Arb10*	chr4	61,708,051	389	392	389	389	409	3
D4mit15*	chr4	111,809,356	215	215	215	215	202	2
D5mit18	chr5	17,062,312	235	219	225	235	231	4
D5wox11	chr5	138,180,823	137	123	137	137	137	2
D6rat105	chr6	19,888,103	229	225	204	227	227	4
D6mit1*	chr6	99,273,921	134	122	112	134	134	3
D6wox12	chr6	138,065,848	127	127	135	127	127	2
D7wox14	chr7	102,588,256	120	126	122	118	124	5
D7wox24	chr7	115,250,056	133	133	133	133	129	2
D7mgh3*	chr7	123,632,617	233	243	233	243	233	2
D8wox4*	chr8	50,529,563	131	119	129	131	117	4
D8rat14*	chr8	108,068,142	169	161	161	161	169	2
D8wox11	chr8	116,873,553	183	191	181	191	183	3
D9mit2*	chr9	71,771,288	185	185	190	190	185	2
D9rat110	chr9	102,531,710	146	158	169	158	146	3
D10rat199	chr10	1,381,054	190	188	188	188	188	2
D10wox12	chr10	56,220,298	95	95	95	91	91	2
D10mit12*	chr10	94,240,320	142	144	144	144	144	2
D11wox3*	chr11	60,397,957	189	183	183	185	181	4
D11mgh3*	chr11	69,649,708	150	166	186	145	145	4
D11wox2	chr11	80,357,513	152	146	148	146	148	3
D12mit2*	chr12	22,650,702	187	215	187	206	196	4
D12rat36	chr12	37,521,884	205	202	205	188	190	4
D13rat60	chr13	26,919,274	131	125	125	127	127	3
D13rat131	chr13	83,263,753	149	151	149	134	134	3
D14rat72	chr14	8,058,678	152	181	162	179	152	4
ALB*	chr14	19,191,541	136	120	136	133	136	3
D14wox14	chr14	22,009,849	122	112	122	108	108	3
D14rat39	chr14	77,239,784	240	248	240	248	240	2
D15rat55	chr 15	211,024	210	228	228	228	228	2
D15rat123	chr15	59,651,151	136	132	130	122	120	5
D16wox9*	chr16	18,740,885	145	137	191	145	145	3
D16rat15	chr16	80,316,290	142	133	144	133	144	3
D17rat78	chr17	42,541,872	151	157	155	155	155	3
D17wox12*	chr17	63,994,211	136	132	115	119	115	4
D18wox7	chr18	15,539,551	148	142	189	193	189	4
TILP*	chr18	38,177,441	262	240	262	242	242	3
D18rat116	chr18	40,057,808	219	223	219	223	225	3
D18rat8	chr18	71,569,072	155	165	157	165	161	4
D19wox8	chr19	24,455,726	124	126	111	124	111	3
D19wox9	chr19	42,099,630	154	158	158	156	160	4
D20wox3*	chr20	4,855,475	116	110	120	128	114	5
D20mit4	chr20	36,923,763	215	201	192	198	215	4
Dxwox6*	chrX	28,435,436	171	138	173	138	175	4
DXmgh9	chrX	70,352,120	120	146	146	146	120	2
纯合度 homozygosity	/	/	100%	100%	100%	100%	100%	/

染色体 Chromosome	STR标记组合 STR marker combinations	染色体 Chromosome	STR标记组合 STR marker combinations
chr1	D1wox18^#、D1wox21^&、D1mgh14^&	chr11	D11wox3^&、D11wox2^#
chr2	D2rat3^&、D2mgh26^#、D2Arb20^&	chr12	D12mit2^&、D12rat36^&
chr3	D3wox9^&、RH94469^#	chr13	D13rat60^#、D13rat131^#
chr4	D4wox27^#、D4Arb10^#	chr14	D14rat72^&、D14wox14^#
chr5	D5mit18^&、D5wox11*	chr15	D15rat55*、D15rat123^†
chr6	D6rat105^&、D6mit1^#	chr16	D16wox9^#、D16rat15^#
chr7	D7wox14^†、D7mgh3*	chr17	D17rat78^#、D17wox12^&
chr8	D8wox4^&、D8wox11^#	chr18	D18wox7^&、D18rat8^&
chr9	D9mit2*、D9rat110^#	chr19	D19wox8^#、D19wox9^&
chr10	D10rat199、D10mit12	chr20	D20wox3^†、D20mit4^&