›› 1998, Vol. 18 ›› Issue (1): 19-22.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

SCID小鼠的繁育及其在肿瘤研究中初步应用

  

  1. 1.上海市肿瘤研究所,上海 200032;2.上海第二医科大学瑞金医院;3.上海医药工业研究院
  • 收稿日期:1997-12-10 出版日期:1998-01-25 发布日期:2013-03-19

Breeding of SCID Mice and Its Preliminary Application in Cancer Research

  1. 1 Shanghai Cancer Institute,Shanghai 200032;2Shanghai Second University;3Shanghai Institute of Pharmaceutical Industry
  • Received:1997-12-10 Online:1998-01-25 Published:2013-03-19

摘要: 采用SPF屏障系统繁育SCID小鼠377胎共1631只,两种不同的繁育方法比较结果证明1雄×2雌交配法优于1雄×1雌交配法。SCID小鼠离乳后的体重增长,雄性大于雌性,3~8周龄增重较快,8周龄后体重增长趋缓。经寄生虫和微生物检测,符合SPF小鼠标准。将人体肿瘤H128小细胞肺癌、SGC7901胃腺癌、U251脑瘤、BEL7402和SMMC7721肝癌移植于SCID小鼠和BALB/c裸小鼠皮下,发现SCID小鼠的成瘤率和瘤体积明显高于BALB/c裸小鼠,SMMC7721肝癌在SCID小鼠身上出现局部浸润、肝转移和淋巴结转移,发生率分别为46.43%、32.14%和39.29%。SGC7901胃腺癌在SCID小鼠胃原位移植,不仅原位100%生长,还伴有向肝、脾等脏器的高转移。

关键词: SCID小鼠, 裸小鼠, 繁殖, 人体肿瘤, 肿瘤移植, 肿瘤转移

Abstract: SPF SCID mice breeding colony has been maintained successfully in the barrier sys-tem. The reproductive performances and growth of SCID mice were observed. The results showed that the weaning litter sizes were 3. 8±1.3(1♂×1♀)and 4.4±1.6(1♂×2♀),life spans were over 12 months. The microbiological quality monitoring showed that the SCID mice were specific pathogens free. The human tumor cells,including lung cancer (H128),stomach adenocarcinoma (SGC-7901),brain cancer (U251) and hepatocellular carcinoma (BEL-7402,SMMC-7721),were transplanted successfully onto these SCID mice.The results showed that the human tumor grew much better in SCID mice as compared with those in BALB/c -nu mice. Moreover, metastasis were observed in SCID mice with SMMC-7721 and SGC-7901 transplantaion,especially the later with a high metastatic rate in the liver,lymph node,peritoneum and spleen.

Key words: SCID mice, Breeding performance, Human tumor, Transplantation, Metastasis